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Production and Characterization of Extracellular Chitin Deacetylase from Absidia corymbifera DY-9

  • Biochemistry
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Abstract

Recent attention has been paid to the screening of chitin deacetylase (CDA) from fungi, which may be applied into industrial production of chitosan and chitosan oligomers. We screened an extracellular CDA from a fungus that was further identified to be Absidia corymbifera DY-9. CDA from A. Corymbifera DY-9 exhibited a higher enzyme activity than that of Mucor rouxii CDA. The highest yield of CDA was achieved in a liquid medium containing 2% powder chitin at pH 9.0, 28°C and 150 rpm. Moreover, the biochemical properties of this enzyme were studied. It was found that the optimal pH and temperature were 6.5 and 55°C. The enzyme activity could be enhanced in the presence of 1–10 mM Co2+, Ca2+ and Mg2+ while inhibited by acetate and EDTA. The enzyme was active on WSCT-50, glycol chitin, chitosan (DDA 71–88 %) and chitin oligosaccharides with a DP no less than 2.

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Authors and Affiliations

  1. Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnololgy, Institute of Agricultural Science and Technology, Chonnam National University, 500-757, Gwangju, Republic of Korea

    Yong Zhao, Young Ju Kim, Kyung Taek Oh, Nam Van Nguyen & Ro Dong Park

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  1. Yong Zhao
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  2. Young Ju Kim
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  3. Kyung Taek Oh
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  4. Nam Van Nguyen
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  5. Ro Dong Park
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Correspondence to Ro Dong Park.

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Zhao and Kim contributed equally.

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Zhao, Y., Kim, Y.J., Oh, K.T. et al. Production and Characterization of Extracellular Chitin Deacetylase from Absidia corymbifera DY-9. J. Korean Soc. Appl. Biol. Chem. 53, 119–126 (2010). https://doi.org/10.3839/jksabc.2010.021

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  • DOI: https://doi.org/10.3839/jksabc.2010.021

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