Abstract
Astragals membranaceus (A. membranaceus) has been widely used as food material as well as medicinal ingredients for therapeutic effects. The objective of this study was to determine physiological activities such as polyphenol content, SOD-like activity, tyrosinase activity, nitrite scavenging activity and EDA (electron donating ability) of 2-and 6 -year root A. membranaceus extracts. SOD-like activities of all samples were shown the high effects (over 86.63%), and 6-year root A. membranaceus was higher (1.90–5.52%) than 2-year root A. membranaceus. Nitrite Scavenging activity (pH1.2) of 6 year root A. membranaceus were higher (9.08–13.42%) than 2 year root A. membranaceus extracts, and 50% ethanol extracts of samples were highest. EDAs of 2-year root A. membranaceus extracts were higher (26.76–44.46%) than 6-year root extracts, while those extracts were lower (42.66–74.05%) than 1.0% and 0.1% L-ascorbate solutions. Tyrosinase inhibitory effects and total polyphenol contents of 6 year root A. membranaceus extracts were higher than 2 year root A. membranace (1.90–5.52%), while both samples were lower than 1.0% and 0.1% L-ascorbate solutions. 6-year root A. membranaceus tended to have higher antioxidant effects of these antioxidant experiments than 2-year root A. membranaceus. Therefore, these suggest that the extracts of A. membranaceus can be used as a data to develop more efficient material in functional food.
Similar content being viewed by others
Abbreviations
- A. membranaceus :
-
Astragals membranaceus
- EDA:
-
electron donating activity
- NSA:
-
nitrite-scavenging activity
- ROS:
-
reactive oxygen species
- SOD:
-
superoxide dismutase
References
Arnous A, Makris DP, and Kefakas P (2001) Effect of principal polyphenolic components in relation antioxidant characteristics of aged red winds. J Agr Food Chem 49, 5736–5742.
Blios MS (1958) Antioxidant determination by the use of a stable free radical. Nature 26, 1199–1200.
Choi J, Shin MH, Park KY, Lee KT, Jung HJ, Park HJ (2003) Effect of tectorigenin obtained from Pueraria thunbergiana flowers on Phase I, II enzyme activities in the Streoptozotocin-induced diabetic rat. Natural Prod Sci 9, 235–240
Chung KS, Kiatm JY, Hong SW, and Lee BK (2006) Isolated of bacteria producing a β-cell-specific biological response modifier found in Korean fermented soybean past. J Microbiol Biotechn 16, 126–135
Chung S, Kim N, and Yoon S (1999) Nitrite scavenging effect of methanol fraction obtained from green yellow vegetable juices. J Korean Soc Food Sci Nutr 28, 342–347.
Folin O and Denis W (1912) On phosphotungastic-phosphomolybdic compounds as color reagents. J Biol Chem 12, 239–243.
Gray JI and Dugan Jr. LR (1975) Inhibition of N-nitrosamine formation in model food system. J Food Sci 40, 981–984.
He ZQ and John A (1991) Constituents of Astragalus membranaceus. J Nat Prod 54, 810–815.
Jeon JR and Choi. JH (2007) Protective effect of water extract of Fraxinus Rhynchophylla leaves on acetaminophen-induced nephrotoxicity in mice and its phenolic compounds. Food Sci Biotechnol 16, 988–993.
Jiang CK, Jeong KJ, Park DK, Pail HD, Yoon YC, and Lee SK (2008) Antioxidant effects of Cheonggukjang containing Phellinus liteus Extract. Food Sci Biotechnol 17, 85–89
Jung S, Lee N, Kim SJ, and Han D (1995) Screening of tyrosinase inhibitor from plants. Korean J Food Sci Technol 27, 891–896.
Kang JH (2004) Modification of Cu,Zn-superoxide dimutase by oxidezed catecholamines. J Biochem and Molecular Bio 37, 325–329.
Kang YH, Park YK, and Lee GD (1996) The nitrite scavenging and electron donating ability of phenolic compounds. Korean J Food Sci Technol 28, 232–239.
Kim SJ, Han D, Moon KD, and Rhee JS (1995) Measurement of superoxide dismutase-like activity of natural antioxidants. Biosci Biotech Biochem 59, 822–826.
Kim SM, Cho YS, and Sung SK (2001) The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J Food Sci Technol 33, 626–632.
Kim YC, Kim MY, Yoshiaki T, Masatake N, and Chung SK (2007) Phenolic antioxidants isolated from mulberry leaves. Food Sci Biotechnol 16, 854–857.
Mirivish SS, Wallcave L, Eagen M, and Shubik P (1972) Ascorbate nitrite reaction: Possible means of the formation of carcinogenic N-nirtoso compounds. Science 177, 65–67.
Nice DJ, Robinson DS, and Holden MA (1995) Characterization of a heat-stable antioxidant co-purified with the superoxide dismutase activity from dried peas. Food Chem 52, 393–397.
Shen HH, Wang K, Li Wen, Ying YH, Gao GX, Li XB, and Huang HQ (2007) Astragalus Membranaceus prevents airway hyperreactivity in mice related to the response inhibition. J Ethnopharm 116, 363–369.
Sheng BW, Chen XF, Zhao J, He DI, and Nan XY (2005) Astragalus membranaceus reduces free radical-mediated injury to renal tubules in rabbits receiving high-energy shock waves. China Med J 1, 43–49.
Simic MG (1988) Mechanisms of inhibition of free-radical processed in mutagenesis and carcinogenesis. Mutat Res 202, 377–386.
Wong TC, Luh BS, and Whitaker JR (1971) Isolation and characterization of polyphenol oxidase isozymes of clingstone peach. Plant Physiol 48, 19–23.
Yim MH, Hong TG, and Lee JH (2006) Antioxidant and antimicrobial activities of fermentation and ethanol extracts of pine needles. Food Sci Biotechnol 15, 582–588.
Yoo MA, Kim JS, Chung HK, Park WJ, and Kang MH (2007) The Antioxidant activity of various cultivars of grape skin extract. Food Sci Biotechnol 16, 884–888.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kim, J.Y., Do, JR., Kwon, JH. et al. Antioxidant effects of Astragalus membranaceus extracts with extraction conditions. J. Korean Soc. Appl. Biol. Chem. 52, 702–707 (2009). https://doi.org/10.3839/jksabc.2009.116
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.3839/jksabc.2009.116