Skip to main content
Log in

Antioxidant effects of Astragalus membranaceus extracts with extraction conditions

  • Published:
Journal of the Korean Society for Applied Biological Chemistry Submit manuscript

Abstract

Astragals membranaceus (A. membranaceus) has been widely used as food material as well as medicinal ingredients for therapeutic effects. The objective of this study was to determine physiological activities such as polyphenol content, SOD-like activity, tyrosinase activity, nitrite scavenging activity and EDA (electron donating ability) of 2-and 6 -year root A. membranaceus extracts. SOD-like activities of all samples were shown the high effects (over 86.63%), and 6-year root A. membranaceus was higher (1.90–5.52%) than 2-year root A. membranaceus. Nitrite Scavenging activity (pH1.2) of 6 year root A. membranaceus were higher (9.08–13.42%) than 2 year root A. membranaceus extracts, and 50% ethanol extracts of samples were highest. EDAs of 2-year root A. membranaceus extracts were higher (26.76–44.46%) than 6-year root extracts, while those extracts were lower (42.66–74.05%) than 1.0% and 0.1% L-ascorbate solutions. Tyrosinase inhibitory effects and total polyphenol contents of 6 year root A. membranaceus extracts were higher than 2 year root A. membranace (1.90–5.52%), while both samples were lower than 1.0% and 0.1% L-ascorbate solutions. 6-year root A. membranaceus tended to have higher antioxidant effects of these antioxidant experiments than 2-year root A. membranaceus. Therefore, these suggest that the extracts of A. membranaceus can be used as a data to develop more efficient material in functional food.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

A. membranaceus :

Astragals membranaceus

EDA:

electron donating activity

NSA:

nitrite-scavenging activity

ROS:

reactive oxygen species

SOD:

superoxide dismutase

References

  • Arnous A, Makris DP, and Kefakas P (2001) Effect of principal polyphenolic components in relation antioxidant characteristics of aged red winds. J Agr Food Chem 49, 5736–5742.

    Article  CAS  Google Scholar 

  • Blios MS (1958) Antioxidant determination by the use of a stable free radical. Nature 26, 1199–1200.

    Article  Google Scholar 

  • Choi J, Shin MH, Park KY, Lee KT, Jung HJ, Park HJ (2003) Effect of tectorigenin obtained from Pueraria thunbergiana flowers on Phase I, II enzyme activities in the Streoptozotocin-induced diabetic rat. Natural Prod Sci 9, 235–240

    CAS  Google Scholar 

  • Chung KS, Kiatm JY, Hong SW, and Lee BK (2006) Isolated of bacteria producing a β-cell-specific biological response modifier found in Korean fermented soybean past. J Microbiol Biotechn 16, 126–135

    CAS  Google Scholar 

  • Chung S, Kim N, and Yoon S (1999) Nitrite scavenging effect of methanol fraction obtained from green yellow vegetable juices. J Korean Soc Food Sci Nutr 28, 342–347.

    Google Scholar 

  • Folin O and Denis W (1912) On phosphotungastic-phosphomolybdic compounds as color reagents. J Biol Chem 12, 239–243.

    CAS  Google Scholar 

  • Gray JI and Dugan Jr. LR (1975) Inhibition of N-nitrosamine formation in model food system. J Food Sci 40, 981–984.

    Article  CAS  Google Scholar 

  • He ZQ and John A (1991) Constituents of Astragalus membranaceus. J Nat Prod 54, 810–815.

    Article  CAS  Google Scholar 

  • Jeon JR and Choi. JH (2007) Protective effect of water extract of Fraxinus Rhynchophylla leaves on acetaminophen-induced nephrotoxicity in mice and its phenolic compounds. Food Sci Biotechnol 16, 988–993.

    CAS  Google Scholar 

  • Jiang CK, Jeong KJ, Park DK, Pail HD, Yoon YC, and Lee SK (2008) Antioxidant effects of Cheonggukjang containing Phellinus liteus Extract. Food Sci Biotechnol 17, 85–89

    CAS  Google Scholar 

  • Jung S, Lee N, Kim SJ, and Han D (1995) Screening of tyrosinase inhibitor from plants. Korean J Food Sci Technol 27, 891–896.

    Google Scholar 

  • Kang JH (2004) Modification of Cu,Zn-superoxide dimutase by oxidezed catecholamines. J Biochem and Molecular Bio 37, 325–329.

    Article  CAS  Google Scholar 

  • Kang YH, Park YK, and Lee GD (1996) The nitrite scavenging and electron donating ability of phenolic compounds. Korean J Food Sci Technol 28, 232–239.

    Google Scholar 

  • Kim SJ, Han D, Moon KD, and Rhee JS (1995) Measurement of superoxide dismutase-like activity of natural antioxidants. Biosci Biotech Biochem 59, 822–826.

    Article  CAS  Google Scholar 

  • Kim SM, Cho YS, and Sung SK (2001) The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J Food Sci Technol 33, 626–632.

    Google Scholar 

  • Kim YC, Kim MY, Yoshiaki T, Masatake N, and Chung SK (2007) Phenolic antioxidants isolated from mulberry leaves. Food Sci Biotechnol 16, 854–857.

    CAS  Google Scholar 

  • Mirivish SS, Wallcave L, Eagen M, and Shubik P (1972) Ascorbate nitrite reaction: Possible means of the formation of carcinogenic N-nirtoso compounds. Science 177, 65–67.

    Article  Google Scholar 

  • Nice DJ, Robinson DS, and Holden MA (1995) Characterization of a heat-stable antioxidant co-purified with the superoxide dismutase activity from dried peas. Food Chem 52, 393–397.

    Article  CAS  Google Scholar 

  • Shen HH, Wang K, Li Wen, Ying YH, Gao GX, Li XB, and Huang HQ (2007) Astragalus Membranaceus prevents airway hyperreactivity in mice related to the response inhibition. J Ethnopharm 116, 363–369.

    Article  Google Scholar 

  • Sheng BW, Chen XF, Zhao J, He DI, and Nan XY (2005) Astragalus membranaceus reduces free radical-mediated injury to renal tubules in rabbits receiving high-energy shock waves. China Med J 1, 43–49.

    Google Scholar 

  • Simic MG (1988) Mechanisms of inhibition of free-radical processed in mutagenesis and carcinogenesis. Mutat Res 202, 377–386.

    Article  CAS  Google Scholar 

  • Wong TC, Luh BS, and Whitaker JR (1971) Isolation and characterization of polyphenol oxidase isozymes of clingstone peach. Plant Physiol 48, 19–23.

    Article  CAS  Google Scholar 

  • Yim MH, Hong TG, and Lee JH (2006) Antioxidant and antimicrobial activities of fermentation and ethanol extracts of pine needles. Food Sci Biotechnol 15, 582–588.

    Google Scholar 

  • Yoo MA, Kim JS, Chung HK, Park WJ, and Kang MH (2007) The Antioxidant activity of various cultivars of grape skin extract. Food Sci Biotechnol 16, 884–888.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Hyun-Ku Kim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, J.Y., Do, JR., Kwon, JH. et al. Antioxidant effects of Astragalus membranaceus extracts with extraction conditions. J. Korean Soc. Appl. Biol. Chem. 52, 702–707 (2009). https://doi.org/10.3839/jksabc.2009.116

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.3839/jksabc.2009.116

Key words

Navigation