JoVE Journal
Biology
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Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)
13.9K Views
•
11:09 min
•
January 2nd, 2015
DOI :
January 2nd, 2015
•Chapters in this video
0:05
Title
1:46
Deposition on the MALDI Target
2:38
Mass Spectra Acquisition Using FlexControl Software
3:56
Database Construction Using Bionumerics Software
6:41
Mass Spectrum Data Analysis
8:24
Bacteria Identification with a Custom Database
9:40
Results: Similarity-coefficient-based Method Can Correctly Identify Bacteria to the Species Level
10:33
Conclusion
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