针对现有非侵入式聚焦技术存在计算复杂、抗噪性能低、优化速度慢等问题，提出一种基于波前整形的非侵入式散射介质内聚焦技术。该技术利用荧光目标的线性激发特性，通过最大化荧光散斑的强度和对比度的线性组合，利用空间光调制器优化外部输入激光的波前相位，实现输入光在散射介质内的聚焦。实验结果表明，该方法实现了散射介质内对单个目标粒子的聚焦，且具有收敛速度快、抗噪性强的特点，为复杂介质内的无创成像提供了一种新的实现途径，并有望在生命科学领域得到广泛应用。

The existing non-invasive focusing technology has the problems of complex calculation, low anti-noise performance and slow optimization speed. Therefore, a wavefront shaping-based method is proposed. Based on the linear excitation of fluorescence, by maxmizing the linear combination of the intensity and contrast of the detected fluorescent speckle in an appropriate contribution, a phase-only spatial light modulator is used to optimize the input light so as to realize the focusing in the scattering medium. The experimental results show that the proposed method not only realizes deep focusing on a single particle in the scattering medium, but also has a fast convergence speed and is insensitive to noise. Moreover, it provides a new way for non-invasive deep imaging in complex medium and is expected to be used in life science.