光学相干层析成像(OCT)技术具有非接触、分辨率高、采集速度快等优势,不仅能够显示样品的三维结构,而且能够检测样品中的运动信息。构建了高分辨率光谱OCT血管成像系统,系统横向分辨率约为6.7 μm,纵向分辨率约为4.7 μm,相机线采集速度140 kHz,能够在2 s内完成三维扫描。在此基础上,通过相邻B扫描图像差分运算提取血流信息,实现血管成像。应用该系统扫描了大鼠大脑皮层的三维血管网络,实验结果表明,系统具有对毛细血管成像的能力。

Optical coherence tomography (OCT) is a non-contact imaging modality with advantages of high resolution and high acquisition speed. OCT can provide three-dimensional image structures and detect motion information in the samples. In this study, a high-resolution spectral OCT angiography system was constructed with the lateral and axial resolutions of about 6.7 μm and about 4.7 μm, respectively. The line scan camera was operated at 140 kHz and could complete a three-dimensional scan within 2 s. Based on this, the blood flow perfusion information was extracted by differentiating the adjacent B-scan images. A micro-vasculature network image of rat cerebral cortex was obtained. The experimental results demonstrate that the proposed system is suitable for capillary imaging.