Abstracts Open Access
Copyright ©The Author(s) 1998. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 15, 1998; 4(Suppl2): 139-139
Published online Oct 15, 1998. doi: 10.3748/wjg.v4.iSuppl2.139
Blood clot as a DNA source for studying genetic polymorphism of human carcinogen-metabolizing enzymes
Gang Liu, Qi Zhou, Li-Dong Wang, Yong-Yu Wang, Jian-Xiang Zou, Laboratory for Cancer Research, Henan Medical University, Zhengzhou 450052, Henan Province, China
Jun-Yan Hong, Cheng-Jun Deng, Laboratory for Cancer Research, College of Pharmacy, Rutgers University, Piscataway, NJ 08854, United States
Author contributions: All authors contributed equally to the work.
Supported by National Natural Science Foundation of China 39670296 and 39770296.
Correspondence to: Dr. Gang Liu, Laboratory for Cancer Research, Henan Medical University, Zhengzhou 450052, Henan Province, China
Received: July 19, 1998
Revised: August 17, 1998
Accepted: September 9, 1998
Published online: October 15, 1998

Abstract

AIM: Genetic polymorphism of human carcinogen-metabolizing enzymes such as cytochromes p450 (CYP) and glutathione S-transferases (GST), may cause alterations of enzyme activity and affect an individuals ability to metabolize environmental carcinogens. In cancer epidemiological and interventional studies, serum from cancer patients and non-cancer subjects are often discarded. The present study was undertaken to investigate the possibility of using blood clots as a DNA source for PCR-based genetic polymorphism analysis on carcinogen- metabolizing enzymes.

METHODS: Twenty blood samples were obtained from healthy subjects in Henan, China. The blood clots were stored in -80 °C prior to use. PCR-bas ed identification was performed in comparison to the use of purified DNA.

RESULTS: The DNA yield from 4 clot samples ranged from 13 to 9 6 μg/mL clots. Successful polymorphism analysis of CYP2E1 (Pst 1, Rsa 1, and Dra 1), GSTM1, and GSTT1 was demonstrated for all 20 samples examined. The concordance rate of PCR-based identification was 100% for direct use of clot lysate in comparison to the use of purified DNA.

CONCLUSION: We conclude that the blood clot is a valuable DNA source for genetic polymorphism analysis. Concerning with the existing cancer epidemiological studies, this convenient DNA source provides a good opportunity to determine the relationship between genetic polymorphism and cancer susceptibility.

Key Words: Cytochromes p450, Glutathione S-transferase, Genetic polymorphism, DNA, Carcinogen metabolizing enzymes



Footnotes

E- Editor: Li RF

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