色谱

色谱 ›› 2013, Vol. 31 ›› Issue (12): 1189-1193.DOI: 10.3724/SP.J.1123.2013.06029

• 研究论文 • 上一篇    下一篇

高效液相色谱-串联质谱法同时测定烟草中5种Amadori化合物

王红瑞1,2, 谢媛媛2, 梁琼麟2, 王义明2, 胡坪1, 罗国安1,2   

  1. 1. 华东理工大学药学院, 上海 200237;
    2. 清华大学化学系, 北京 100084
  • 收稿日期:2013-06-14 修回日期:2013-09-08 出版日期:2013-12-28 发布日期:2013-12-05
  • 通讯作者: 罗国安，谢媛媛
  • 基金资助:

    中国烟草总公司特色优质烟叶开发重大专项（TS-05-20110034）

Simultaneous determination of five Amadori compounds in tobacco by high performance liquid chromatography-mass spectrometry

WANG Hongrui1,2, XIE Yuanyuan2, LIANG Qionglin2, WANG Yiming2, HU Ping1, LUO Guoan1,2   

  1. 1. School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;
    2. Department of Chemistry, Tsinghua University, Beijing 100084, China
  • Received:2013-06-14 Revised:2013-09-08 Online:2013-12-28 Published:2013-12-05

PDF

191

被引次数

7

摘要：

建立了高效液相色谱-三重四极杆质谱（HPLC-QqQ MS）联用定量分析烟草中5种Amadori前香物质的方法。样品经含5%（v/v）甲醇的水提取后，采用Waters XBridgeTM Amide色谱柱（250 mm×4.6 mm，3.5 μm）、以甲醇-水为流动相进行分离，采用多反应监测（MRM）模式测定烟草样品中5种Amadori化合物的含量。结果表明，5种Amadori化合物的峰面积与质量浓度的线性关系良好，相关系数r为0.9895~0.9989；定量限（S/N=10）在10.18~44.58 μg/L，检出限（S/N=3）在3.51~14.86 μg/L；平均加标回收率为92.6%~123.6%，相对标准偏差（RSD）小于9.4%。本方法操作简便、灵敏度高、分析速度快，可以用于烟叶及卷烟中5种主要Amadori类物质的含量测定。

关键词: Amadori化合物, Maillard反应, 高效液相色谱-串联质谱, 前香物, 烟草

Abstract:

A method of high performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-QqQ MS) in multiple reaction monitoring (MRM) mode for simultaneous determination of five Amadori compounds in tobacco has been developed. The separation was performed on an XBridgeTM Amide column (250 mm×4.6 mm, 3.5 μm) by gradient elution using methanol and water as mobile phases. The linearity of this method was good with the correlation coefficients (r) of 0.9895-0.9989. The limits of detection (S/N=3) were from 3.51 μg/L to 14.86 μg/L, and the limits of quantitation (S/N=10) were from 10.18 μg/L to 44.58 μg/L. The recoveries varied from 92.6% to 123.6% with the relative standard deviations (RSDs) less than 9.4%. The established method is rapid, accurate, robust and convenient, and it could be applied to the measurement of the five Amadori compounds in tobacco and cigarettes.

Key words: Amadori compounds, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), Maillard reaction, precursor aroma, tobacco

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