色谱

色谱 ›› 2013, Vol. 31 ›› Issue (5): 435-440.DOI: 10.3724/SP.J.1123.2012.12047

• 研究论文 • 上一篇    下一篇

应用二极管阵列串联荧光检测-高效液相色谱技术快速测定玩具材料中蒽油的4种成分

何乔桑1, 鹿燕1, 廖上富1, 李秀娣2*   

  1. 1. 浙江省质量检测科学研究院, 浙江 杭州 310013;
    2. 杭州市标准化研究院, 浙江 杭州 310005
  • 收稿日期:2012-12-28 修回日期:2013-03-01 出版日期:2013-05-28 发布日期:2013-05-16
  • 通讯作者: 李秀娣,Tel:(0571)85379011,E-mail:lixiudihz@163.com.
  • 基金资助:

    浙江省质量技术监督系统科研计划项目(20100402).

Determination of four polycyclic aromatic hydrocarbons in anthracene oil for toys by high performance liquid chromatography with diode array detection and fluorescence detection

HE Qiaosang1, LU Yan1, LIAO Shangfu1, LI Xiudi2*   

  1. 1. Zhejiang Institute of Quality Inspection Science, Hangzhou 310013, China;
    2. Hangzhou Institute of Standardization, Hangzhou 310005, China
  • Received:2012-12-28 Revised:2013-03-01 Online:2013-05-28 Published:2013-05-16

PDF

140

被引次数

3

摘要：

采用二极管阵列串联荧光检测-高效液相色谱法对玩具材料中蒽油的4种主要成分蒽、菲、芘、荧蒽进行了检测。样品用正己烷-丙酮溶液超声提取,硅胶小柱净化。方法的荧光检测线性范围为0.5～1000 μg/L,二极管阵列检测线性范围为0.5～1000 mg/L。加标回收、精密度试验表明4种成分的回收率为70.0%～120%,精密度为0.7%～8.8%。蒽、菲、芘、荧蒽的检出限分别为0.1、0.1、0.2、0.3 μg/L,定量限分别为0.4、0.2、0.5、0.8 μg/L。方法具有灵敏度高、检出限低的特点,适用于玩具材料中蒽油4种成分的快速定性、定量检测。

关键词: 多环芳烃, 蒽油, 二极管阵列检测, 高效液相色谱, 玩具, 荧光检测

Abstract:

A method using high performance liquid chromatography with diode array detection coupled with fluorescence detection was applied to determine four polycyclic aromatic hydrocarbons in anthracene oil for toys. This method showed good abilities of the baseline separation and high sensitivity under the optimized analytical conditions. The limits of the fluorescence detection were 0.5-1000 μg/L, while the limits of the diode array detection were 0.5-1000 mg/L. The precisions of the method were 0.7%-8.8%. The recoveries of the standard samples were 70.0%-120%. The limits of detection (LOD) of anthracene, pyrene, phenanthrene and fluoranthene were 0.1, 0.1, 0.2 and 0.3 μg/L, and the limits of quantitation (LOQ) of the four substances were 0.4, 0.2, 0.5 and 0.8 μg/L, respectively. In comparison with other methods, the method has higher detection speed and lower detection limits, which is useful to analyze rapidly those four polycyclic aromatic hydrocarbons in anthracene oil for toys.

Key words: anthracene oil, diode array detection, fluorescence detection, high performance liquid chromatography (HPLC), polycyclic aromatic hydrocarbons, toys

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