FIRST REPORT IN IRAQ : AMINO ACID SUBSTITUTION IN PMRCAB GENES AND THERE CORELLATION WITH COLISTIN RESISTANCE AMONG A.BAUMANNII ISOLATES
DOI:
https://doi.org/10.36103/ijas.v53i2.1530Keywords:
colistin, antimicrobial resistant, mutation.Abstract
In the present study,out of 120 samples were collected from different clinical sites from patients who admitted to different hospitals in Baghdad city,only 65 samples were detected as A. baumannii.The antibiotics susceptibility test showed that the bacteria were resistant to piperacillin (92.31%), cefotaxime (87.69%),trimethoprimsulfamethoxazole(73.85%).While Meropenem (70.77%)and gentamycin(67.69 %).The resistance rate to imipenem and tetracycline were 58.46% and 43.08% respectively. Eventually, just(20%)among all isolates are resistant to levofloxacin. While the percentage of colistin resistance was performed among 23 isolates and the results showed that all isolates were reported resistance in a percentage 100% with MIC values ≥ 16 µg/100µl.The results of pmrCAB genes detection and sequencing among seven A.baumannii isolates, which were choices because they were resistant to all antibiotics even colistin, were showed that the entire region of the pmrC containing fourteen missense mutations. Out of twelve detected mutations in pmrA, only one of them was found to exhibit a missense mutations. While the results of pmrB gene showed three missenses mutations.
References
Evans,B.A;A.Hamouda,andS.G.B.Amyes.2013.The rise of carbapenem -resistant Acinetobacter baumannii. Current Pharmaceutical Design,19(2): 223-238
Abdallah, E. M ; F. Ahamed, and A.S. Al-Omari . 2015. Antibiotic susceptibility patterns of some clinical isolates from al-Rass general hospital. International Journal of Biosciences, 6(9): 47-54
Ahmed, M. U., R. Farooq, N. Al-Hawashim, M. Ahmed, N. Yiannakou, F. Sayeed, and S. Lutfullah . 2015. Sensitive, resistant and multi-drug resistant Acinetobacter baumanii at Saudi Arabia hospital eastern region. Pak J Pharm Sci, 28:825-832
Al-Haideri, H. H. 2019. Gene expression of blaOXA-51-like and blaOXA-23 in response to β-lactam antibiotic in clinically isolated Acinetobacter baumannii and Acinetobacter lowffii from urine samples . Iraqi Journal of Agricultural Sciences,50(40).
Aliakbarzade, K; S. Farajnia, A.K. Nik, F.Zarei, and A.Tanomand . 2014. Prevalence of aminoglycoside resistance genes in Acinetobacter baumannii isolates. Jundishapur Journal of Microbiology, 7(10).
Al-Shaabani M. J. M ;A .M .T .Al-Ethawi, and H.J.F.AL-Mathkhury. 2020. Eco-frienly synthesis of gold nanoparticles and study their effect with antibiotics against Acinetobacter baumannii. Iraqi Journal of Agricultural Sciences, 51(4): 1204-1211
Aly, M. M;N.A. Alsoud, M.S. Elrobh, S.M. Al Johani, and H.H Balkhy. 2016. High prevalence of the PER-1 gene among carbapenem-resistant Acinetobacter baumannii in Riyadh, Saudi Arabia. European Journal of Clinical Microbiology and Infectious Diseases, 35(11):1759-1766
Beheshti, M; M. Talebi, A. Ardebili, A. Bahador, and A.R. Lari . 2014. Detection of AdeABC efflux pump genes in tetracycline-resistant Acinetobacter baumannii isolates from burn and ventilator-associated pneumonia patients. Journal of Pharmacy and Bioallied Sciences, 6(4), 229
Boinett, C. J; A.K.Cain, J. Hawkey, N.T. Do Hoang, N.N.T. Khanh, D.P. Thanh, and G.C. Langridge . 2019. Clinical and laboratory-induced colistin-resistance mechanisms in Acinetobacter baumannii. Microbial Genomics, 5(2).
Cheung, J; and W.A. Hendrickson . 2010. Sensor domains of two-component regulatory systems. Current Opinion in Microbiology, 13(2): 116-123.
Clinical and Laboratory Standards Institute. 2017. Performance standards for antimicrobial susceptibility testing. CLSI Supplement M100-S30
Dafopoulou, K; B.B.Xavier, A. Hotterbeekx, L. Janssens, C. Lammens, E. Dé, and S. Pournaras . 2016. Colistin-resistant Acinetobacter baumannii clinical strains with deficient biofilm formation. Antimicrobial Agents and Chemotherapy, 60(3): 1892-1895
Eliopoulos, G. M; L.L. Maragakis, and T.M. Perl . 2008. Acinetobacter baumannii: epidemiology, antimicrobial resistance, and treatment options. Clinical Infectious Diseases, 46(8): 1254-1263
Ghaima, K. K. 2018. Distribution of extended spectrum beta-lactamase (ESBL) genes among Acinetobacter baumannii isolated from burn infections. MOJ Cell Sci Rep, 5(2) :42-46
Hameed, F;M.A. Khan, H. Muhammad, T. Sarwar, H.Bilal, and T.U. Rehman. 2019. Plasmid-mediated mcr-1 gene in Acinetobacter baumannii and Pseudomonas aeruginosa: first report from Pakistan. Revista da Sociedade Brasileira de Medicina Tropical, 52
Hamza and H. Yaaqoob. 2020.Evaluation the effect of green synthesis titanium dioxide nanoparticles on Acinetobacter baumannii isolates. Iraqi Journal of Agricultural Sciences , 51(6): 1486-1495.
Higgins, P. G ; H. Wisplinghoff, D. Stefanik, and H. Seifert. 2004. Selection of topoisomerase mutations and overexpression of adeB mRNA transcripts during an outbreak of Acinetobacter baumannii. Journal of antimicrobial chemotherapy, 54(4): 821-823
Huang, J;C. Li, J. Song, T. Velkov, L. Wang, Y. Zhu, and J. Li . 2020. Regulating polymyxin resistance in Gram-negative bacteria: roles of two-component systems PhoPQ and PmrAB. Future Microbiology, 15(6):445-459
Lee, C. R;J. H. Lee, M. Park, K.S. Park, I.K. Bae, Y.B.Kim, and S.H. Lee . 2017. Biology of Acinetobacter baumannii: pathogenesis, antibiotic resistance mechanisms, and prospective treatment options. Frontiers in Cellular and Infection Microbiology, 7, 55
Lescat, M; L. Poirel, A. Jayol, and P. Nordmann . 2019. Performances of the rapid polymyxin Acinetobacter and Pseudomonas tests for colistin susceptibility testing. Microbial Drug Resistance, 25(4) :520-523
Li, J;C.R. Rayner, R.L. Nation, R. Deans, R. Boots, N. Widdecombe, and J.Lipman . 2005. Pharmacokinetics of colistin methanesulfonate and colistin in a critically ill patient receiving continuous venovenous hemodiafiltration. Antimicrobial Agents and Chemotherapy, 49(11): 4814-4815
Liu, Y. Y; C.E.Chandler, L.M. Leung, C.L. McElheny, R.T. Mettus, R.M. Shanks, and Y. Doi . 2017. Structural modification of lipopolysaccharide conferred by mcr-1 in Gram-negative ESKAPE pathogens. Antimicrobial Agents and Chemotherapy, 61(6).
Lucas, D. D; B. Crane, A.Wright, M.L. Han, J. Moffatt, D. Bulach, and D. Machado. 2018. Emergence of high-level colistin resistance in an Acinetobacter baumannii clinical isolate mediated by inactivation of the global regulator H-NS. Antimicrobial Agents and Chemotherapy, 62(7).
Luo, S. C ; Y.C. Lou, M. Rajasekaran, Y.W. Chang, C.D. Hsiao, and C. Chen. 2013. Structural basis of a physical blockage mechanism for the interaction of response regulator PmrA with connector protein PmrD from Klebsiella pneumoniae. Journal of Biological Chemistry, 288(35): 25551-25561
Malinverni, J. C; and T.J. Silhavy . 2009. An ABC transport system that maintains lipid as mmetry in the gram-negative outer membrane. Proceedings of the National Academy of Sciences, 106(19): 8009-8014
Mera, R. M; L.A. Miller, H. Amrine-Madsen, and D.F. Sahm . 2010. Acinetobacter baumannii 2002–2008: increase of carbapenem-associated multiclass resistance in the United States. Microbial Drug Resistance, 16(3): 209-215
Mettus, R. 2019. Identification of colistin resistance mechanisms in pmrC-, pmrA-, and pmrB-deficient Acinetobacter baumannii .Ph.D thesis.PP:7
Mshachal, M. A ; T.R. Abdulrahman, M.S. Khudair, J.S. and Hassan . 2017. Molecular detection of multidrug resistant Acinetobacter baumannii from different clinical samples. Iraqi Journal of Medical Sciences, 15(3): 314-323
Nurtop, E; F. Bayındır Bilman, S. Menekse, O. Kurt Azap, M. Gönen, O. Ergonul, and F. Can. 2019. Promoters of colistin resistance in Acinetobacter baumannii infections. Microbial Drug Resistance, 25(7): 997-1002
Oikonomou, O; S. Sarrou, C.C. Papagiannitsis, S. Georgiadou, K. Mantzarlis, E. Zakynthinos, and E. Petinaki . 2015. Rapid dissemination of colistin and carbapenem resistant Acinetobacter baumannii in Central Greece: mechanisms of resistance, molecular identification and epidemiological data. BMC Infectious Diseases, 15(1): 559
Pagal, S;R. Saranathan, A.Rani, A. Tomar, K.P Arunkumar, and K. Prashanth . 2019. Paucity of adaptive selection in PmrAB two-component system may resist emergence of colistin resistance in Acinetobacter baumannii. bioRxiv, 753665
Poirel, L; R.A. Bonnin, and P. Nordmann . 2011. Genetic basis of antibiotic resistance in pathogenic Acinetobacter species. IUBMB Life, 63(12): 1061-1067
Samantha, A; and A. Vrielink .2020 . Lipid A Phosphoethanolamine Transferase: Regulation, Structure and Immune Response. Journal of Molecular Biology, 432(18) :5184-5196
Tiwari, V; N. Mishra, K. Gadani, P.S. Solanki, N.A. Shah, and M. Tiwari. 2018. Mechanism of anti-bacterial activity of zinc oxide nanoparticle against carbapenem-resistant Acinetobacter baumannii. Frontiers in Microbiology, 9, 1218
Vashist, J;V. Tiwari, R. Das, A. Kapil and M.R. Rajeswari . 2011. Analysis of penicillin-binding proteins (PBPs) in carbapenem resistant Acinetobacter baumannii. The Indian Journal of Medical Research, 133(3): 332
Yezli, S;A.M. Shibl, and Z.A. Memish. 2015. The molecular basis of β-lactamase production in Gram-negative bacteria from Saudi Arabia. Journal of Medical Microbiology, 64(2): 127-136.1.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
2.jpg)
2.jpg)
