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Vet Comp Orthop Traumatol 2015; 28(03): 172-178
DOI: 10.3415/VCOT-14-06-0088
Original Research
Schattauer GmbH

Effect of locally injected autologous platelet-rich plasma on second intention wound healing of acute full-thickness skin defects in dogs

M. Karayannopoulou
1   Department of Clinical Studies, Companion Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece
,
D. Psalla
2   Laboratory of Pathology, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece
,
G. Kazakos
1   Department of Clinical Studies, Companion Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece
,
P. Loukopoulos
2   Laboratory of Pathology, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece
,
N. Giannakas
1   Department of Clinical Studies, Companion Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece
,
I. Savvas
1   Department of Clinical Studies, Companion Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece
,
M. Kritsepi-Konstantinou
3   Department of Clinical Studies, Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece
,
A. Chantes
4   Department of Plastic Surgery, Medical School, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece
,
L. G. Papazoglou
1   Department of Clinical Studies, Companion Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece
› Author Affiliations
Further Information

Publication History

Received:12 June 2014

Accepted:16 February 2015

Publication Date:
28 December 2017 (online)

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Summary

Objectives: Second intention wound healing may be impaired by wound and host factors and thus more advanced therapies are required for a fast and satisfactory outcome. Platelet-rich plasma (PRP), rich in growth factors and cytokines essential for tissue repair, could improve wound healing. The purpose of this experimental study was to evaluate the effect of locally injected autologous PRP on second intention healing of acute full-thickness skin defects in dogs.

Methods: Three 2x2 cm full-thickness skin defects were created bilaterally on the dorso-lateral area of the trunk of six Beagle dogs. The wounds of one randomly selected side received PRP treatment, whereas the contra-lateral wounds were left untreated (controls). Wound healing was evaluated by planimetry, laser-Doppler flowmetry measurements of tissue perfusion, and histo-logically.

Results: The rate of wound healing did not differ significantly between the two groups. Tissue perfusion was significantly higher in the PRP-treated group (p = 0.008) compared to controls on day 10. Histological evaluation revealed a trend towards greater collagen production and a significantly better collagen orientation (p = 0.019) in PRP-treated wounds on day 20.

Clinical significance: Locally injected auto-logous PRP does not accelerate the healing process, but increases tissue perfusion and may promote the formation of organized collagen bundles in acute full-thickness skin defects in dogs.

Keywords

Dog - full-thickness skin defect - platelet-rich plasma - second intention wound healing
 

  • References

  • 1 Beldon P. Basic science of wound healing. Surgery 2010; 28: 409-412.
    PubMedGoogle Scholar
  • 2 Schreml S, Szeimies R-M, Prantl L. et al. Wound healing in the 21st century. J Am Acad Dermatol 2010; 63: 866-881.
    CrossrefPubMedGoogle Scholar
  • 3 Fossum TW. Small Animal Surgery. 4th ed. St. Louis, Missouri: Mosby Elsevier; 2013: 190-207.
    Google Scholar
  • 4 Bohling MW, Henderson RA, Swaim SF. et al. Comparison of the role of the subcutaneous tissues in cutaneous wound healing in the dog and cat. Vet Surg 2006; 35: 3-14.
    CrossrefPubMedGoogle Scholar
  • 5 Li J, Chen J, Kirsner R. Pathophysiology of acute wound healing. Clin Dermatol 2007; 25: 9-18.
    CrossrefPubMedGoogle Scholar
  • 6 Lacci KM, Dardik A. Platelet-rich plasma: support for its use in wound healing. Yale J Biol Med 2010; 83: 1-9.
    PubMedGoogle Scholar
  • 7 Behm B, Babilas P, Landthaler M. et al. Cytokines, chemokines and growth factors in wound healing. J Eur Acad Dermatol Venereol 2012; 26: 812-820.
    CrossrefPubMedGoogle Scholar
  • 8 Faler BJ, Macsata RA, Plummer D. et al. Transforming growth factor-β and wound healing. Perspect Vasc Surg Endovasc Ther 2006; 18: 55-62.
    CrossrefPubMedGoogle Scholar
  • 9 Barrientos S, Stojadinovic O, Golinko MS. et al. Growth factors and cytokines in wound healing. Wound Rep Reg 2008; 16: 585-601.
    CrossrefPubMedGoogle Scholar
  • 10 Hardwicke J, Schmaljohann D, Boyce D. et al. Epidermal growth factor therapy and wound healing - past, present and future perspectives. Surgeon 2008; 3: 172-177.
    PubMedGoogle Scholar
  • 11 Bao P, Kodra A, Tomic-Canic M. et al. The role of vascular endothelial growth factor in wound healing. J Surg Res 2009; 153: 347-358.
    CrossrefPubMedGoogle Scholar
  • 12 Cole BJ, Seroyer ST, Filardo G. et al. Platelet-rich plasma: where are we now and where are we going?. Sports Health 2010; 2: 203-210.
    CrossrefPubMedGoogle Scholar
  • 13 Hom DB, Linzie BM, Huang TC. The healing effects of autologous platelet gel on acute human skin wounds. Arch Facial Plast Surg 2007; 9: 174-183.
    PubMedGoogle Scholar
  • 14 Silva RF, Carmona JU, Rezende CMF. Intra-articular injections of autologous platelet concentrates in dogs with surgical reparation of cranial cruciate ligament rupture. Vet Comp Orthop Traumatol 2013; 26: 285-290.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 15 Lee H-W, Reddy MS, Geurs N. et al. Efficacy of platelet-rich plasma on wound healing in rabbits. J Periodontol 2008; 79: 691-696.
    CrossrefPubMedGoogle Scholar
  • 16 Kazakos K, Lyras DN, Verettas D. et al. The use of autologous PRP gel as an aid in the management of acute trauma wounds. Injury 2009; 40: 801-805.
    CrossrefPubMedGoogle Scholar
  • 17 Pallua N, Wolter T, Markowicz M. Platelet-rich plasma in burns. Burns 2010; 36: 4-8.
    CrossrefPubMedGoogle Scholar
  • 18 Yang HS, Shin J, Bhang SH. et al. Enhanced skin wound healing by sustained release of growth factors contained in platelet-rich plasma. Exp Mol Med 2011; 43: 622-629.
    CrossrefPubMedGoogle Scholar
  • 19 Dionyssiou D, Demiri E, Foroglou P. et al. The effectiveness of intralesional injection of platelet-rich plasma in accelerating the healing of chronic ulcers. An experimental and clinical study. Int Wound J 2013; 10: 397-406.
    CrossrefPubMedGoogle Scholar
  • 20 Carter MJ, Fylling CP, Parnell KS. Use of platelet rich plasma gel on wound healing: a systematic review and meta-analysis. ePlasty 2011; 11: 382-410.
    PubMedGoogle Scholar
  • 21 Carter CA, Jolly DG, Worden Sr. CE. et al. Platelet-rich plasma gel promotes differentiation and regeneration during equine wound healing. Exp Mol Pathol 2003; 74: 244-255.
    CrossrefPubMedGoogle Scholar
  • 22 Monteiro SO, Lepage OM, Theoret CL. Effects of platelet-rich plasma on the repair of wounds on the distal aspect of the forelimb in horses. Am J Vet Res 2009; 70: 277-282.
    CrossrefPubMedGoogle Scholar
  • 23 Sardari K, Reza Emami M, Kazemi H. et al. Effects of platelet-rich plasma (PRP) on cutaneous regeneration and wound healing in dogs treated with dexamethasone. Comp Clin Pathol 2011; 20: 155-162.
    CrossrefPubMedGoogle Scholar
  • 24 Tambella AM, Attili AR, Dini F. et al. Autologous platelet gel to treat chronic decubital ulcers: A randomized, blind controlled clinical trial in dogs. Vet Surg 2014; 43: 726-733.
    PubMedGoogle Scholar
  • 25 Kim J-H, Park C, Park H-M. Curative effect of autologous platelet-rich plasma on a large cutaneous lesion in a dog. Vet Dermatol 2009; 20: 123-126.
    CrossrefPubMedGoogle Scholar
  • 26 Manning TO, Monteiro-Riviere NA, Bristol DG. et al. Cutaneous laser-Doppler velocimetry in nine animal species. Am J Vet Res 1991; 52: 1960-1964.
    PubMedGoogle Scholar
  • 27 Gillette RL, Swaim SF, Sartin EA. et al. Effects of a bioactive glass on healing of closed skin wounds in dogs. Am J Vet Res 2001; 62: 1149-1153.
    CrossrefPubMedGoogle Scholar
  • 28 Timmenga EJF, Andreassen TT, Houthoff HJ. et al. The effect of mechanical stress on healing skin wounds: an experimental study in rabbits using tissue expansion. Br J Plast Surg 1991; 44: 514-519.
    CrossrefPubMedGoogle Scholar
  • 29 Eppley BL, Pietrzak WS, Blanton M. Platelet-rich plasma: a review of biology and applications in plastic surgery. Plast Reconstr Surg 2006; 118: 147e-159e.
    CrossrefPubMedGoogle Scholar
  • 30 Alsousou J, Thompson M, Hulley P. et al. The biology of platelet-rich plasma and its application in trauma and orthopaedic surgery. J Bone Joint Surg Br 2009; 91 B 987-996.
    PubMedGoogle Scholar
  • 31 Eppley BL, Woodell JE, Higgins J. Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing. Plast Reconstr Surg 2004; 114: 1502-1508.
    PubMedGoogle Scholar
  • 32 Weibrich G, Kleis WKG, Hafner G. et al. Growth factor levels in platelet-rich plasma and correlations with donor age, sex, and platelet count. J Craniomaxillofac Surg 2002; 30: 97-102.
    CrossrefPubMedGoogle Scholar
  • 33 Yan YH, Larson DL. Acceleration of full-thickness wound healing in porcine model by autologous platelet gel. Wounds 2007; 19: 79-86.
    PubMedGoogle Scholar
  • 34 Mehta S, Watson JT. Platelet rich concentrate: basic science and current clinical applications. J Orthop Trauma 2008; 22: 433-438.
    PubMedGoogle Scholar
  • 35 Crovetti G, Martinelli G, Issi M. et al. Platelet gel for healing cutaneous chronic wounds. Transf Apheres Sci 2004; 30: 145-151.
    PubMedGoogle Scholar
  • 36 Provost PJ, Baily JV. Principles of plastic and reconstructive surgery. In Auer JA, Stick JA. editors Equine Surgery. 4th ed. St. Louis, Missouri: Elsevier-Saunders; 2012: 271-284.
    Google Scholar
  • 37 Kumar V, Abbas AK, Fausto N. Tissue renewal and repair: Regeneration, healing, and fibrosis. In: Robbins and Cotran Pathological Basis of Disease. 7th ed. Philadelphia: Elsevier-Saunders; 2005: 87-118.
    Google Scholar
  • 38 Ackermann MR. Chronic inflammation and wound healing. In McGavin MD, Zachary JF. editors Pathological Basis of Veterinary Disease. 4th ed. St. Louis, Missouri: Elsevier-Mosby; 2007: 153-191.
    Google Scholar
  • 39 Ashrafpour H, Huang N, Neligan PC. et al. Vasodilator effect and mechanism of action of vascular endothelial growth factor in skin vasculature. Am J Physiol-Heart C 2004; 286: H946-954.
    PubMedGoogle Scholar
  • 40 van Hengel T, ter Haar G, Kirpensteijn J. Wound management: a new protocol for dogs and cats. In Kirpensteijn J, ter Haar G. editors Reconstructive Surgery and Wound Management of the Dog and Cat. London, UK: Manson Publishing; 2013: 21-48.
    Google Scholar
  • 41 AL-Bayati AH, Al-Asadi RN, Mahadi AK. et al. Effects of autologous platelets rich plasma on full-thickness cutaneous wounds healing in goats. Int J Anim Veter Adv 2013; 5: 233-239.
    PubMedGoogle Scholar