The Promotion of Humoral Immune Responses in Humans via SOCS1-Mediated Th2-Bias Following SARS-CoV-2 Vaccination
Abstract
:1. Introduction
2. Materials and Methods
2.1. Clinical Sample Collection and Preparation
2.2. Serum Antibody Concentration Detection
2.3. Sample Preparation for Single-Cell Sequencing
2.4. Single-Cell Sequencing and Data Processing
2.5. Cell Type Annotations
2.6. Identification of Signature Genes of the Cell Clusters
2.7. Quantification and Statistical Analyses
3. Results
3.1. Single-Cell Expression Atlas of PBMCs before and after Vaccination
3.2. Single-Cell Transcriptional Profiles of T Cells after Vaccination Revealed Differential T Cell Subclusters between Different Humoral Immune Response Groups
3.3. The Expression Atlas of Treg Cells Was Different between the Two Groups
3.4. The DEGs of the Treg Cells Correlated with Humoral Immune Responses
3.5. The CD4+ Cell Subcluster with High Expression of LncRNA MALAT1 and NEAT1 Was Associated with Enhanced Humoral Immune Responses
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Pre-Vaccination | Post-Vaccination | ||||
---|---|---|---|---|---|
ID | Group | nCoVnAbs (AU/mL) | nCoVIgG (S/CO) | nCoVnAbs (AU/mL) | nCoVIgG (S/CO) |
C71 | C | 5.095 | 0.080 | 40.646 | 1.091 |
C744 | C | 36.01 | 0.158 | 0.906 | 14.526 |
C199 | C | 9.52 | 0.034 | 30.924 | 3.565 |
E735 | E | 22.764 | 1.661 | 1450.328 | 57.69 |
E921 | E | 23.956 | 1.536 | 3782.458 | 148.56 |
E774 | E | 30.283 | 1.343 | 875.019 | 37.705 |
Sample | Group | Ratio—Pre (%) | Mean—Pre (%) | Ratio—Post (%) | Mean—Post (%) |
---|---|---|---|---|---|
C199E | C | 0.038 | 0.015 | ||
C71E | C | 0.030 | 0.037 | ||
C744E | C | 0.016 | 0.028 | 0.019 | 0.024 |
E735E | E | 0.026 | 0.024 | ||
E774E | E | 0.009 | 0.017 | ||
E921E | E | 0.018 | 0.018 | 0.024 | 0.022 |
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Liu, X.; Han, J.; Cui, R.; Peng, M.; Song, H.; Li, R.; Chen, G. The Promotion of Humoral Immune Responses in Humans via SOCS1-Mediated Th2-Bias Following SARS-CoV-2 Vaccination. Vaccines 2023, 11, 1730. https://doi.org/10.3390/vaccines11111730
Liu X, Han J, Cui R, Peng M, Song H, Li R, Chen G. The Promotion of Humoral Immune Responses in Humans via SOCS1-Mediated Th2-Bias Following SARS-CoV-2 Vaccination. Vaccines. 2023; 11(11):1730. https://doi.org/10.3390/vaccines11111730
Chicago/Turabian StyleLiu, Xiaoyu, Junyong Han, Renjie Cui, Meifang Peng, Huaidong Song, Rui Li, and Gang Chen. 2023. "The Promotion of Humoral Immune Responses in Humans via SOCS1-Mediated Th2-Bias Following SARS-CoV-2 Vaccination" Vaccines 11, no. 11: 1730. https://doi.org/10.3390/vaccines11111730