Improvement of Carrot Accelerated Solvent Extraction Efficacy Using Experimental Design and Chemometric Techniques
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Accelerated Solvent Extraction Procedure
2.3. Total Carotenoid Contents (TCar)
2.4. Total Polyphenol Content (TPh)
2.5. Radical Scavenging Activity (SA) by DPPH● Assay
2.6. Reducing Power (RP)
2.7. Experimental Design and Response Surface Methodology (RSM)
2.8. Hierarchical Cluster Analysis (HCA) and Sum of Ranking Differences (SRD)
3. Results and Discussion
3.1. RSM Analysis of the Extraction Experiments
3.2. HCA of the Extraction Experiments
3.3. SRD Analysis of the Extraction Experiments
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Experiment | Solvent Volume Fraction (v/v) | εm | RPm | ρm (g/dm3) | P′m | ||
Ethanol | Acetone | Water | |||||
---|---|---|---|---|---|---|---|
(a) Organic solvent and water mixtures | |||||||
1 | 0.8 | 0 | 0.2 | 82.77 | 0.72 | 772.91 | 5.96 |
2 | 0 | 0.8 | 0.2 | 24.66 | 0.48 | 769.01 | 6.12 |
3 | 0 | 0.8 | 0.2 | 24.66 | 0.48 | 769.01 | 6.12 |
4 | 0.2 | 0.6 | 0.2 | 39.19 | 0.54 | 769.99 | 6.08 |
5 | 0.4 | 0.4 | 0.2 | 53.72 | 0.60 | 770.96 | 6.04 |
6 | 0.6 | 0.2 | 0.2 | 68.24 | 0.66 | 771.94 | 6.00 |
7 | 0.8 | 0 | 0.2 | 82.77 | 0.72 | 772.91 | 5.96 |
8 | 0.4 | 0.4 | 0.2 | 53.72 | 0.60 | 770.96 | 6.04 |
Opt1 | 0.31 | 0.49 | 0.2 | 47.18 | 0.58 | 770.53 | 6.06 |
Experiment | Solvent Volume Fraction (v/v) | εm | RPm | ρm (g/dm3) | P′m | ||
Ethanol | Acetone | Water | |||||
(b) Organic solvent mixtures | |||||||
9 | 1 | 0 | 0 | 88.21 | 0.65 | 725.63 | 5.20 |
10 | 0 | 1 | 0 | 15.57 | 0.36 | 720.76 | 5.40 |
11 | 0 | 1 | 0 | 15.57 | 0.36 | 720.76 | 5.40 |
12 | 0.25 | 0.75 | 0 | 33.73 | 0.43 | 721.98 | 5.35 |
13 | 0.5 | 0.5 | 0 | 51.89 | 0.50 | 723.20 | 5.30 |
14 | 0.75 | 0.25 | 0 | 70.05 | 0.58 | 724.42 | 5.25 |
15 | 1 | 0 | 0 | 88.21 | 0.65 | 725.63 | 5.20 |
16 | 0.5 | 0.5 | 0 | 51.89 | 0.50 | 723.20 | 5.30 |
Opt2 | 1 | 0 | 0 | 88.21 | 0.654 | 725.634 | 5.20 |
Experiment | TCar (mg/100 g) | TPh (mg/100 g) | SA (μmol TE/100 g) | RP (μmol TE/100 g) |
---|---|---|---|---|
1 | 3 ± 0 a | 131 ± 5 f,g,h | 980 ± 37 e | 205 ± 8 i |
2 | 17 ± 1 d | 123 ± 5 f,g | 710 ± 30 c | 149 ± 6 f |
3 | 13 ± 1 c,d | 117 ± 5 f | 514 ± 21 a | 170 ± 7 f,g |
4 | 8 ± 0 b | 127 ± 6 f,h | 575 ± 23 a,b | 183 ± 7 g,h |
5 | 5 ± 0 a | 133 ± 6 g,h | 671 ± 29 b | 189 ± 6 g,h |
6 | 3 ± 0 a | 142 ± 7 h | 783 ± 34 c,d | 185 ± 8 g,h |
7 | 3 ± 0 a | 121 ± 6 f | 943 ± 46 d,e | 190 ± 9 h,i |
8 | 5 ± 0 a | 141 ± 6 h | 776 ± 30 c,d | 176 ± 7 g |
9 | 21 ± 1 e | 115 ± 5 e,f | 1920 ± 86 g | 171 ± 6 g |
10 | 14 ± 1 d | 56 ± 2 a | 720 ± 29 c | 55 ± 2 a,b |
11 | 12 ± 1 c | 77 ± 3 b | 1330 ± 55 f | 67 ± 2 b |
12 | 23 ± 1 e,f | 59 ± 2 a | 1020 ± 42 e | 108 ± 4 c |
13 | 23 ± 1 e | 83 ± 3 b,c,d | 824 ± 37 d | 41 ± 1 a |
14 | 27 ± 2 f | 94 ± 4 c,d | 888 ± 37 d | 155 ± 6 e,f |
15 | 22 ± 1 e | 101 ± 5 d,e | 1960 ± 79 g | 142 ± 7 e |
16 | 25 ± 1 f | 96 ± 4 c,d | 759 ± 26 b,c,d | 126 ± 4 d |
TCar a | TPh b | SA c | RP c | Acetone | Ethanol | Water | |
---|---|---|---|---|---|---|---|
Ethanol + Acetone + 20% Water | |||||||
Single response-TCarmax | 14.750 | − | − | − | 0.8 | 0 | 0.2 |
Single response-TPhmax | − | 137.708 | − | − | 0.34 | 0.46 | 0.2 |
Single response-SAmax | − | − | 922.416 | − | − | 0.8 | 0.2 |
Single response-RPmax | − | − | − | 197.982 | − | 0.8 | 0.2 |
Multi response | 6.407 | 135.695 | 703.899 | 176.811 | 0.49 | 0.31 | 0.2 |
Ethanol + Acetone | |||||||
Single response-TCarmax | 25.8774 | − | − | − | 0.366 | 0.634 | − |
Single response-TPhmax | − | 107.394 | − | − | 0 | 1 | − |
Single response-SAmax | − | − | 1870.27 | − | 0 | 1 | − |
Single response-RPmax | − | − | − | 155.744 | 0 | 1 | − |
Multi response | 21.6177 | 107.394 | 1870.394 | 155.744 | 0 | 1 | − |
Source | Sum of Squares | Degree of Freedom | Mean Square | F-Value | p-Value |
---|---|---|---|---|---|
Total Carotenoids | |||||
Model | 182.23 | 2 | 91.12 | 51.81 | 0.0005 |
Residual | 8.79 | 5 | 1.76 | ||
Lack of fit | 0.46 | 2 | 0.23 | 0.083 | 0.9228 |
Pure error | 8.33 | 3 | 2.78 | ||
Total | 191.02 | 7 | |||
R2 = 0.9540 | |||||
Total Polyphenols | |||||
Model | 420.07 | 2 | 210.04 | 6.34 | 0.0426 |
Residual | 165.74 | 5 | 33.15 | ||
Lack of fit | 66.45 | 2 | 33.23 | 1.00 | 0.4636 |
Pure error | 99.28 | 3 | 33.09 | ||
Total | 1529.87 | 7 | |||
R2 = 0.7171 | |||||
Scavenging Activity | |||||
Model | 1.433 × 105 | 1 | 1.433 × 105 | 20.22 | 0.0041 |
Residual | 42,513.88 | 6 | 7085.65 | ||
Lack of fit | 17,103.52 | 3 | 5701.17 | 0.67 | 0.6236 |
Pure error | 25,410.36 | 3 | 8470.12 | ||
Total | 1.858 × 105 | 7 | |||
R2= 0.7712 | |||||
Reducing Power | |||||
Model | 1345.02 | 1 | 1345.02 | 13.42 | 0.0105 |
Residual | 601.35 | 6 | 100.22 | ||
Lack of fit | 175.36 | 3 | 58.45 | 0.41 | 0.7574 |
Pure error | 425.99 | 3 | 142.00 | ||
Total | 1946.37 | 7 | |||
R2= 0.6910 |
Source | Sum of Squares | Degree of Freedom | Mean Square | F-Value | p-Value |
---|---|---|---|---|---|
Total Carotenoids | |||||
Model | 181.48 | 2 | 90.74 | 29.69 | 0.0017 |
Residual | 15.28 | 5 | 3.06 | ||
Lack of fit | 9.11 | 2 | 4.56 | 2.21 | 0.2566 |
Pure error | 6.17 | 3 | 2.06 | ||
Total | 196.77 | 7 | |||
R2 = 0.9223 | |||||
Total Polyphenols | |||||
Model | 2258.84 | 1 | 2258.84 | 19.16 | 0.0047 |
Residual | 707.35 | 6 | 117.89 | ||
Lack of fit | 312.30 | 3 | 104.10 | 0.79 | 0.5743 |
Pure error | 395.05 | 3 | 131.68 | ||
Total | 2966.19 | 7 | |||
R2 = 0.7615 | |||||
Scavenging Activity | |||||
Model | 1.453 × 106 | 2 | 7.266 × 105 | 10.68 | 0.0157 |
Residual | 3.403 × 105 | 5 | 68,066.93 | ||
Lack of fit | 1.517 × 105 | 2 | 75,858.05 | 1.21 | 0.4126 |
Pure error | 1.886 × 105 | 3 | 62,872.85 | ||
Total | 1.794 × 106 | 7 | |||
R2= 0.8102 | |||||
Reducing Power | |||||
Model | 10,233.03 | 1 | 10,233.03 | 9.46 | 0.0218 |
Residual | 6489.54 | 6 | 1081.59 | ||
Lack of fit | 2340.70 | 3 | 780.23 | 0.56 | 0.6751 |
Pure error | 4148.84 | 3 | 1382.95 | ||
Total | 16,722.57 | 7 | |||
R2= 0.6119 |
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Tumbas Šaponjac, V.; Kovačević, S.; Šeregelj, V.; Šovljanski, O.; Mandić, A.; Ćetković, G.; Vulić, J.; Podunavac-Kuzmanović, S.; Čanadanović-Brunet, J. Improvement of Carrot Accelerated Solvent Extraction Efficacy Using Experimental Design and Chemometric Techniques. Processes 2021, 9, 1652. https://doi.org/10.3390/pr9091652
Tumbas Šaponjac V, Kovačević S, Šeregelj V, Šovljanski O, Mandić A, Ćetković G, Vulić J, Podunavac-Kuzmanović S, Čanadanović-Brunet J. Improvement of Carrot Accelerated Solvent Extraction Efficacy Using Experimental Design and Chemometric Techniques. Processes. 2021; 9(9):1652. https://doi.org/10.3390/pr9091652
Chicago/Turabian StyleTumbas Šaponjac, Vesna, Strahinja Kovačević, Vanja Šeregelj, Olja Šovljanski, Anamarija Mandić, Gordana Ćetković, Jelena Vulić, Sanja Podunavac-Kuzmanović, and Jasna Čanadanović-Brunet. 2021. "Improvement of Carrot Accelerated Solvent Extraction Efficacy Using Experimental Design and Chemometric Techniques" Processes 9, no. 9: 1652. https://doi.org/10.3390/pr9091652