Nitrogen Enhances Salt Tolerance by Modulating the Antioxidant Defense System and Osmoregulation Substance Content in Gossypium hirsutum
Abstract
:1. Introduction
2. Results
2.1. Nitrogen Supplementation Increased Growth and Biomass Accumulation
2.2. Nitrogen Addition Improves Pigment Synthesis
2.3. Influence of Nitrogen on Gas Exchange Attributes in Response to Salinity Stress
2.4. Nitrogen Induces Osmolyte Accumulation under Salt Stress
2.5. Application of Nitrogen Improves the Plant Water Status
2.6. Application of Nitrogen Mitigates Oxidative Damage
2.7. Nitrogen Upregulates the Antioxidant Activity of Cotton Genotypes under Salinity Stress
2.8. Cluster Heat Map Analysis
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Plant Materials, Growth Environments, and Experimental Treatments
5.2. Estimation of Growth Parameters
5.3. Biomass Determination
5.4. Determination of Photosynthetic Pigments
5.5. Measurement of Gas Exchange Attributes
5.6. Determination of Oxidative Stress Markers
5.7. Determination of Soluble Sugar, Soluble Protein, and Free Amino Acid Contents
5.8. Determination of Leaf Relative Water Content
5.9. Assay of Antioxidant Enzymes
5.10. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Sikder, R.K.; Wang, X.; Zhang, H.; Gui, H.; Dong, Q.; Jin, D.; Song, M. Nitrogen Enhances Salt Tolerance by Modulating the Antioxidant Defense System and Osmoregulation Substance Content in Gossypium hirsutum. Plants 2020, 9, 450. https://doi.org/10.3390/plants9040450
Sikder RK, Wang X, Zhang H, Gui H, Dong Q, Jin D, Song M. Nitrogen Enhances Salt Tolerance by Modulating the Antioxidant Defense System and Osmoregulation Substance Content in Gossypium hirsutum. Plants. 2020; 9(4):450. https://doi.org/10.3390/plants9040450
Chicago/Turabian StyleSikder, Ripon Kumar, Xiangru Wang, Hengheng Zhang, Huiping Gui, Qiang Dong, Dingsha Jin, and Meizhen Song. 2020. "Nitrogen Enhances Salt Tolerance by Modulating the Antioxidant Defense System and Osmoregulation Substance Content in Gossypium hirsutum" Plants 9, no. 4: 450. https://doi.org/10.3390/plants9040450