MicroRNA Profiling Revealed the Mechanism of Enhanced Cold Resistance by Grafting in Melon (Cucumis melo L.)
Abstract
:1. Introduction
2. Results
2.1. Grafting with Pumpkin Rootstock Enhanced the Plant Resistance to Low Temperatures in Melon
2.2. Small-RNA Signatures of SG and PG Seedlings under Low-Temperature Stress
2.3. Identification of DEMs during Cold Response
2.4. Expression Patterns of Four Key DEMs under Low-Temperature Stress and Target Gene Prediction
2.5. Expression Pattern Analyses of Key Target Genes under Low-Temperature Stress
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Treatments
4.2. Determination of Physiological Indicators
4.3. Small-RNA Library Construction and Small-RNA Sequencing
4.4. Identification of Known and Novel miRNAs
4.5. Identification of LTR Cis-Acting Elements
4.6. Quantitative Real-Time RT-PCR (qRT-PCR)
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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N-SG1 | N-SG2 | N-SG3 | L-SG1 | L-SG2 | L-SG3 | N-PG1 | N-PG2 | N-PG3 | L-PG1 | L-PG2 | L-PG3 | |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Known miRNAs | 109 | 110 | 105 | 111 | 108 | 108 | 109 | 111 | 111 | 112 | 109 | 107 |
Novel miRNAs | 106 | 105 | 104 | 105 | 104 | 105 | 106 | 104 | 105 | 105 | 105 | 105 |
ID | Rename | Localization | CDS Length (bp) | Best Hits to Arabidopsis | Functional Description in Arabidopsis |
---|---|---|---|---|---|
MELO3C002370 | CmSPL13 | chr12: 24537602–24540376 (+) | 969 | AT5G50570/AtSPL13 | Involved in floral transition |
MELO3C016713 | CmGCR2 | chr07: 2653267–2655968 (−) | 1239 | AT1G52920/AtGCR2 | Encodes a plasma membrane-localized ABA receptor, which interacts with the Gαβγ complex |
MELO3C012858 | CmRIC7 | chr04: 13342065–13344382 (+) | 636 | AT4G28556/AtRIC7 | Encodes RIC7, the downstream effector of active Rop2 GTPase |
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Lang, X.; Zhao, X.; Zhao, J.; Ren, T.; Nie, L.; Zhao, W. MicroRNA Profiling Revealed the Mechanism of Enhanced Cold Resistance by Grafting in Melon (Cucumis melo L.). Plants 2024, 13, 1016. https://doi.org/10.3390/plants13071016
Lang X, Zhao X, Zhao J, Ren T, Nie L, Zhao W. MicroRNA Profiling Revealed the Mechanism of Enhanced Cold Resistance by Grafting in Melon (Cucumis melo L.). Plants. 2024; 13(7):1016. https://doi.org/10.3390/plants13071016
Chicago/Turabian StyleLang, Xinmei, Xuan Zhao, Jiateng Zhao, Tiantian Ren, Lanchun Nie, and Wensheng Zhao. 2024. "MicroRNA Profiling Revealed the Mechanism of Enhanced Cold Resistance by Grafting in Melon (Cucumis melo L.)" Plants 13, no. 7: 1016. https://doi.org/10.3390/plants13071016