iTRAQ-Based Proteomic and Physiological Analyses Reveal the Mechanisms of Dehydration and Cryopreservation Tolerance of Sophora tonkinensis Gagnep. Seeds
Abstract
:1. Introduction
2. Results
2.1. Dehydration Tolerance of S. tonkinensis Seeds
2.2. Effects of Dehydration and Cryopreservation after Dehydration Treatment on the Cell Structure of S. tonkinensis Seed Plumular Axis
2.3. Response of Antioxidant Enzyme Activities in S. tonkinensis Seed Plumular Axis Treated with Dehydration and Dehydration Followed by Cryopreservation Treatment
2.4. Results of iTRAQ Analysis and Differential Protein Screening of S. tonkinensis Seeds Plumular Axis Treated with Dehydration and Dehydration Followed by Cryopreservation Treatment
2.5. KEGG and Go Functional Annotation of DEPs in S. tonkinensis Seeds Axis Treated with Dehydration and Cryopreservation
3. Discussion
3.1. Molecular Mechanism of Dehydration Tolerance in S. tonkinensis Seeds
3.2. Cryopreservation Treatment May Be Used as a Candidate Method for the Preservation of S. tonkinensis Seeds
4. Materials and Methods
4.1. Experimental Materials
4.2. Germination Experiment
4.3. Cell Structure and Antioxidant Enzyme Activity of Seedplumular Axis Treated with Cryopreservation after Dehydration and Dehydration Treatment
4.4. Protein Extraction and Enzyme Hydrolysis
4.5. iTRAQ Labeling and Grouping
4.6. Database Search and Proteomic Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Luo, Y.; Zhang, Y.; Jiang, Y.; Dai, Z.; Li, Q.; Mou, J.; Xu, L.; Deng, S.; Li, J.; Wang, R.; et al. iTRAQ-Based Proteomic and Physiological Analyses Reveal the Mechanisms of Dehydration and Cryopreservation Tolerance of Sophora tonkinensis Gagnep. Seeds. Plants 2023, 12, 1842. https://doi.org/10.3390/plants12091842
Luo Y, Zhang Y, Jiang Y, Dai Z, Li Q, Mou J, Xu L, Deng S, Li J, Wang R, et al. iTRAQ-Based Proteomic and Physiological Analyses Reveal the Mechanisms of Dehydration and Cryopreservation Tolerance of Sophora tonkinensis Gagnep. Seeds. Plants. 2023; 12(9):1842. https://doi.org/10.3390/plants12091842
Chicago/Turabian StyleLuo, Yongjian, Yixin Zhang, Yu Jiang, Zhangyan Dai, Qing Li, Jiaolin Mou, Li Xu, Shiming Deng, Jitao Li, Ru Wang, and et al. 2023. "iTRAQ-Based Proteomic and Physiological Analyses Reveal the Mechanisms of Dehydration and Cryopreservation Tolerance of Sophora tonkinensis Gagnep. Seeds" Plants 12, no. 9: 1842. https://doi.org/10.3390/plants12091842