Patients with Invasive Lobular Carcinoma Show a Significant Increase in IRS-4 Expression Compared to Infiltrative Ductal Carcinoma—A Histopathological Study
Abstract
:1. Introduction
2. Patients and Methods
2.1. Collection of Samples
2.2. Histopathological and Immunohistochemical Studies
2.3. Histopathological Evaluation
2.4. Statistic Analysis
3. Results
3.1. Clinical and Sociodemographic Characteristics of the Study Population
3.2. Patients with Invasive Lobular Carcinoma Show a Significant Increase in the Expression of IRS-4
3.3. Patients with Invasive Lobular Carcinoma Show a Significant Increase in the Expression of C0X-2, Rb1 and Cyclin D1
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Antigen | Dilution | Provider | Protocol Specifications |
---|---|---|---|
IRS-4 | 1:250 | Thermo Fisher Scientific—PA5-117329 | Preincubation with Tris-EDTA buffer pH 9 and incubation with 0.1% TTX (Triton ×100 in TBS) for 5 min. |
COX-2 | 1:750 | Vitro, MAD-000335QD-3/V | ----------------- |
Rb1 | 1:500 | Vitro, MAD-000900QD-3/V | ----------------- |
Cyclin D1 | 1:500 | Vitro, MAD-000630QD-3/V | Preincubation with Tris-EDTA buffer pH 9 and incubation with 0.1% TTX (Triton ×100 in TBS) for 5 min. |
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Ortega, M.A.; Fraile-Martinez, O.; García-Montero, C.; Borja-Vergel, S.; Torres-Carranza, D.; Pekarek, L.; Arribas, C.B.; De León-Luis, J.A.; Sánchez-Rojo, C.; Alvarez-Mon, M.A.; et al. Patients with Invasive Lobular Carcinoma Show a Significant Increase in IRS-4 Expression Compared to Infiltrative Ductal Carcinoma—A Histopathological Study. Medicina 2022, 58, 722. https://doi.org/10.3390/medicina58060722
Ortega MA, Fraile-Martinez O, García-Montero C, Borja-Vergel S, Torres-Carranza D, Pekarek L, Arribas CB, De León-Luis JA, Sánchez-Rojo C, Alvarez-Mon MA, et al. Patients with Invasive Lobular Carcinoma Show a Significant Increase in IRS-4 Expression Compared to Infiltrative Ductal Carcinoma—A Histopathological Study. Medicina. 2022; 58(6):722. https://doi.org/10.3390/medicina58060722
Chicago/Turabian StyleOrtega, Miguel A., Oscar Fraile-Martinez, Cielo García-Montero, Sandra Borja-Vergel, Diego Torres-Carranza, Leonel Pekarek, Coral Bravo Arribas, Juan A. De León-Luis, Cristina Sánchez-Rojo, Miguel Angel Alvarez-Mon, and et al. 2022. "Patients with Invasive Lobular Carcinoma Show a Significant Increase in IRS-4 Expression Compared to Infiltrative Ductal Carcinoma—A Histopathological Study" Medicina 58, no. 6: 722. https://doi.org/10.3390/medicina58060722