DNA Methylation on N6-Adenine Regulates the Hyphal Development during Dimorphism in the Early-Diverging Fungus Mucor lusitanicus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Strains and Culture Conditions
2.2. Disruption of dmt Genes and Generation of Complemented Strains
2.3. Southern Blot
2.4. Experiments of Yeast to Hyphae Transition
2.5. In Vitro Host-Pathogen Interaction Assays
2.6. RNA Extraction and cDNA Synthesis
2.7. RT-qPCR Analysis
2.8. Analysis of 6mA in DNA by HPLC-MS
2.9. Sensitivity Tests
3. Results
3.1. Genes of M. lusitanicus Encoding Putative 6mA Demethylases
3.2. Generation of Single and Double dmt Knockout Mutants in M. lusitanicus
3.3. The dmt1 Is Involved in the Hyphae Formation during the Yeast to Mycelium Transition
3.4. The dmt1 Is Important for the Appropriated pkaR4 Transcription during the Transition from Yeast to Hyphae
3.5. Deletion of dmt1 Alters 6mA Levels during Yeast to Hyphae Transition
3.6. dmt1 Regulates Germination in the Interaction of Spores with Macrophages In Vitro
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Osorio-Concepción, M.; Lax, C.; Navarro, E.; Nicolás, F.E.; Garre, V. DNA Methylation on N6-Adenine Regulates the Hyphal Development during Dimorphism in the Early-Diverging Fungus Mucor lusitanicus. J. Fungi 2021, 7, 738. https://doi.org/10.3390/jof7090738
Osorio-Concepción M, Lax C, Navarro E, Nicolás FE, Garre V. DNA Methylation on N6-Adenine Regulates the Hyphal Development during Dimorphism in the Early-Diverging Fungus Mucor lusitanicus. Journal of Fungi. 2021; 7(9):738. https://doi.org/10.3390/jof7090738
Chicago/Turabian StyleOsorio-Concepción, Macario, Carlos Lax, Eusebio Navarro, Francisco E. Nicolás, and Victoriano Garre. 2021. "DNA Methylation on N6-Adenine Regulates the Hyphal Development during Dimorphism in the Early-Diverging Fungus Mucor lusitanicus" Journal of Fungi 7, no. 9: 738. https://doi.org/10.3390/jof7090738