The Targeted Regulation of BDUbc and BDSKL1 Enhances Resistance to Blight in Bambusa pervariabilis × Dendrocalamopsis grandis
Abstract
:1. Introduction
2. Results
2.1. Bioinformatics Analysis of BDUbc and BDSKL1
2.2. Construction of Overexpression Vectors
2.3. A. tumefaciens Tumefaciens-Mediated Overexpression in the Genetic Transformation of B. pervariabilis × D. grandis
2.3.1. The Genetic Transformation of B. pervariabilis × D. grandis
2.3.2. Identification of Positive Transgenic Seedlings
2.4. Functional Validation of BDUbc and BDSKL1 in Transgenic Plants
2.4.1. The Disease Resistance Level of Transgenic Plants
2.4.2. Changes in BDUbc and BDSKL1 Expression in Transformed Plants during Different Periods of Infestation Detected by qRT-PCR
2.4.3. Physiological and Biochemical Assays
2.5. Subcellular Localization of BDUbc and BDSKL1
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Methods
4.2.1. Bioinformatics Analysis of BDUbc and BDSKL1
4.2.2. Extraction of Total RNA and cDNA Synthesis in B. pervariabilis × D. grandis
4.2.3. Cloning and Vector Construction of BDUbc and BDSKL1
4.2.4. A. tumefaciens Mediated Cultivation of B. pervariabilis × D. grandis Transformants
4.2.5. Verification of Transgenic Plants
4.2.6. Pathogen Infection and Pathogenicity Testing
4.2.7. Changes in BDUbc and BDSKL1 Expression in Transformed Plants during Different Periods of Infestation, as Detected by qRT-PCR
4.2.8. Physiological and Biochemical Measurements
4.2.9. Subcellular Localization of BDUbc and BDSKL1
4.2.10. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yan, P.; Wang, Y.; Yu, C.; Piao, J.; Li, S.; Liu, Y.; Li, S. The Targeted Regulation of BDUbc and BDSKL1 Enhances Resistance to Blight in Bambusa pervariabilis × Dendrocalamopsis grandis. Int. J. Mol. Sci. 2024, 25, 569. https://doi.org/10.3390/ijms25010569
Yan P, Wang Y, Yu C, Piao J, Li S, Liu Y, Li S. The Targeted Regulation of BDUbc and BDSKL1 Enhances Resistance to Blight in Bambusa pervariabilis × Dendrocalamopsis grandis. International Journal of Molecular Sciences. 2024; 25(1):569. https://doi.org/10.3390/ijms25010569
Chicago/Turabian StyleYan, Peng, Yisi Wang, Cailin Yu, Jingmei Piao, Shuying Li, Yinggao Liu, and Shujiang Li. 2024. "The Targeted Regulation of BDUbc and BDSKL1 Enhances Resistance to Blight in Bambusa pervariabilis × Dendrocalamopsis grandis" International Journal of Molecular Sciences 25, no. 1: 569. https://doi.org/10.3390/ijms25010569