Induction of Axonal Outgrowth in Mouse Hippocampal Neurons via Bacterial Magnetosomes
Abstract
:1. Introduction
2. Results and Discussion
2.1. Magnetosome Cell Interactions
2.2. Magnetosomes Induce Stretch-Growth
3. Materials and Methods
3.1. Animals
3.2. Cell Culture
3.3. Chemically Synthesized Nanoparticles
3.4. Cultivation of Magnetospirillum gryphiswaldense
3.5. Magnetosome Isolation and Purification
3.6. Determination of Iron Concentrations
3.7. Fluorescent Labelling of Isolated Magnetosomes
3.8. Sterile Filtration of Magnetosome Suspensions
3.9. Transmission Electron Microscopy
3.10. Analytical Methods
3.11. Bio-Synthetized versus Artificially Synthetized Nanoparticles
3.12. Magnetic Field
3.13. Stretching Assay
3.14. Toxicity Test
3.15. Particle Localization
3.16. Immunostaining
3.17. Image Analysis
3.18. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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SPIONs | Magnetosomes | |
---|---|---|
Core diameter | 75 ± 10 nm | 39.7 ± 8.1 nm |
Hydrodynamic diameter | 100 nm | 51.2 ± 7.2 nm |
Saturation magnetization | 59 Am2 kg−1 | 70–90 Am2 kg−1 |
Coating | Glucuronic acid | Proteinaceous phospholipid membrane |
Concentration for cell labelling | 3.6 µg Fe mL−1 | 1.25–2.5 µg Fe mL−1 |
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De Vincentiis, S.; Falconieri, A.; Mickoleit, F.; Cappello, V.; Schüler, D.; Raffa, V. Induction of Axonal Outgrowth in Mouse Hippocampal Neurons via Bacterial Magnetosomes. Int. J. Mol. Sci. 2021, 22, 4126. https://doi.org/10.3390/ijms22084126
De Vincentiis S, Falconieri A, Mickoleit F, Cappello V, Schüler D, Raffa V. Induction of Axonal Outgrowth in Mouse Hippocampal Neurons via Bacterial Magnetosomes. International Journal of Molecular Sciences. 2021; 22(8):4126. https://doi.org/10.3390/ijms22084126
Chicago/Turabian StyleDe Vincentiis, Sara, Alessandro Falconieri, Frank Mickoleit, Valentina Cappello, Dirk Schüler, and Vittoria Raffa. 2021. "Induction of Axonal Outgrowth in Mouse Hippocampal Neurons via Bacterial Magnetosomes" International Journal of Molecular Sciences 22, no. 8: 4126. https://doi.org/10.3390/ijms22084126