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Review

Current Status of the Degradation of Aliphatic and Aromatic Petroleum Hydrocarbons by Thermophilic Microbes and Future Perspectives

by
Alexis Nzila
Department of Life Sciences, King Fahd University of Petroleum and Minerals Dhahran, PO Box 468, Dhahran 31261, Saudi Arabia
Int. J. Environ. Res. Public Health 2018, 15(12), 2782; https://doi.org/10.3390/ijerph15122782
Submission received: 6 November 2018 / Revised: 30 November 2018 / Accepted: 2 December 2018 / Published: 7 December 2018
(This article belongs to the Section Environmental Health)
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Abstract

:
Contamination of the environment by petroleum products is a growing concern worldwide, and strategies to remove these contaminants have been evaluated. One of these strategies is biodegradation, which consists of the use of microorganisms. Biodegradation is significantly improved by increasing the temperature of the medium, thus, the use of thermophiles, microbes that thrive in high-temperature environments, will render this process more efficient. For instance, various thermophilic enzymes have been used in industrial biotechnology because of their unique catalytic properties. Biodegradation has been extensively studied in the context of mesophilic microbes, and the mechanisms of biodegradation of aliphatic and aromatic petroleum hydrocarbons have been elucidated. However, in comparison, little work has been carried out on the biodegradation of petroleum hydrocarbons by thermophiles. In this paper, a detailed review of the degradation of petroleum hydrocarbons (both aliphatic and aromatic) by thermophiles was carried out. This work has identified the characteristics of thermophiles, and unraveled specific catabolic pathways of petroleum products that are only found with thermophiles. Gaps that limit our understanding of the activity of these microbes have also been highlighted, and, finally, different strategies that can be used to improve the efficiency of degradation of petroleum hydrocarbons by thermophiles were proposed.

1. Introduction

Contamination with petroleum hydrocarbons, as the result of oil exploitation, transport and refinement, is a growing concern worldwide. Thus, their removal from the environment remains a priority. Several approaches exist. However, biodegradation, which exploits the ability of microorganisms to use pollutants as a source of carbon and energy, remains the most attractive, since it is relatively cheaper and environmentally friendlier than chemical and physical approaches.
Petroleum hydrocarbon consists of alkanes (aliphatic compounds with up to 40 or more carbon [C] atoms); monocyclic aromatic hydrocarbons (MAHs, such as benzene, phenol, and their derivatives); polycyclic aromatic hydrocarbons (PAHs), which are subdivided further into low- and high-molecular weight PAHs (LMW-PAHs and HMW-PAHs, respectively). LMW-PAHs consist of 2–3 rings, such as naphthalene (NAPH), phenanthrene (PHEN), anthracene (ANTH), and fluorene (FLR), while HMW-PAHs contain four or more rings, and these include fluoranthene (FLT), pyrene (PYR), benzo[a]pyrene (BZP) and benzo[a]anthracene (BZA) [1,2].
Generally, aliphatic compounds are more amenable to biodegradation than aromatic compounds, and the higher the number of rings, the more difficult is the biodegradation. Nevertheless, the literature is replete with reports on the biodegradation of petroleum hydrocarbons, including HMW-PAHs, in mesophilic conditions. Mesophiles (bacteria that grow optimally at 20–40 °C) that degrade petroleum hydrocarbons have been extensively studied (primarily in low salinity, <4% NaCl, and at neutral pH), and are estimated to belong to more than 65 genera [3], and readers are referred to the following excellent reviews on this topic [2,4,5,6,7,8].
However, contaminations can also occur in extreme environmental conditions, and microorganisms that thrive in these environments are known as extremophiles. These conditions include high salinity (for halophilic microbes), high or low pH (alkalophilic or acidophilic microbes, respectively), high or low temperature (psychrophilic and thermophilic microbes, respectively), and high pressure (barophilic or piezophilic microbes) [9]. In relation to the biodegradation of petroleum hydrocarbons, a sizable body of work has been carried out in hypersaline conditions, and this has been summarized elsewhere [10,11,12].
Thermophiles fall into three groups. The moderate and extreme thermophiles, with optimum biodegradation temperatures of 40–59 °C and 60–85 °C, respectively, and the hyper-thermophiles, with optimum temperatures >85 °C [13,14]. Several advantages are associated with the use of thermophiles (Figure 1). High temperatures lead to increased kinetic reactions (thus, high reaction rates) and high mass transfer, thus increasing the biodegradation. In addition, high temperature has a significant influence on the bioavailability of petroleum hydrocarbons, especially PAHs, by decreasing their viscosity, leading to an increase in their diffusion coefficients, therefore improving their availability to microorganisms [13,14]. All of these properties have made thermophiles a valuable source of enzymes in industrial biotechnology [15,16,17].
As stated earlier, biodegradation of petroleum hydrocarbon has focused on mesophiles, and comparatively little work has been carried out on petroleum hydrocarbon degrading thermophiles (PHDT). Several reviews on PHDT have been published in the last 20 years [18,19,20,21]; however, these reviews lack detailed analyses of the characteristics of PHDT, the mechanisms of degradation, and also comparative information between thermophiles and mesophiles in relation to petroleum product degradation. In the current review, work carried out on this topic since the 1980s was reviewed, and detailed observations of this work (in comparison to that of mesophiles) led to the unravelling of important and unique features of thermophiles involved in the degradation of petroleum hydrocarbons, which have not been described so far. For instance, more than 75% of PHDT belong to one single genus, Geobacillus, a situation different from that which prevails in mesophiles. Unique catabolic pathways (present only in PHDT) were highlighted. More importantly, gaps that limit our understanding of the activity of these microbes have also been presented, and, finally, different strategies that can be used to improve the efficiency of PHDT were proposed. Overall, this review opens up new research avenues on this topic.

2. Summary of Work on PHDT

In 2001, Nazina et al. re-examined the genus Bacillus and proposed a new genus, Geobacillus, for some bacteria that were initially classified as Bacillus. Specifically, those authors proposed that species such as Bacillus stearothermophilus, Bacillus thermoleovorans and Bacillus thermodenitrificans should be classified in the genus Geobacillus, as the renamed species Geobacillus stearothermophilus, Geobacillus thermoleovorans, and Geobacillus thermodenitrificans [22]. Thus, to be in line with this new proposed classification, in this review, the aforementioned Bacillus species were named Geobacillus species even if they were reported before 2001.

2.1. Alkane Biodegradation

The first report on PHDT dates back to the mid-1960s. Mateles et al. isolated a thermophile, Geobacillus stearothermophilus, from soil water/soil samples from the US, by enrichment in a medium containing hydrocarbon at 57 °C (Table 1, ST1) [23]. Almost a decade later, an enrichment experiment on mud samples (from an American littoral zone) at 50 °C, in the presence of n-hexadecane, led to the isolation of a thermophilic microbe, Thermomicrobium fosteri [24] (Table 1, ST2). Further studies showed that this bacterium could utilize n-alkanes (C10–C20), alkenes (C14–C18), alcohols (C12–C17), and ketones (C14–C17). However, compounds of <C9 (n-alkanes, alkenes and ketones) and <C11 (alcohols) were not utilized by this strain [24]. Two years later, a similar study, based on enrichment with heptadecane, led to the isolation of two thermophilic bacterial strains, YS-3 and YS-4, from an inoculum consisting of mud samples from Yellowstone National Park (Table 1, ST3). These strains could degrade C10–C20 n-alkanes and C6–C8 alkenes at an optimum temperature of 60 °C, but not <C9 (alkanes) nor <C6 (alkenes) [25]. Further investigations showed that YS-3 and YS-4 belong to the species Thermoleophilum minutum and Thermoleophilum album, respectively [26]. The inability of these strains to utilize shorter chain n-alkanes will be discussed in Section 3.2. Using a sample from a hot water spring on Kunashir Island, Russia, Loginova et al. isolated two thermophilic bacterial strains, G. stearothermophilus and Thermus ruber, capable of growing in the presence of paraffins at an optimum temperature of 60 °C [27] (ST4). A few years later, in 1984, using an inoculum consisting of mud and water from various sites across the USA, a thermophilic strain, T. album, was isolated by an enrichment experiment at 60 °C, in the presence of n-heptadecane. This strain was capable of degrading C13–C20 n-alkanes [28] (ST5). Three years later (ST6), the same research group, using the same methodology and the same inoculum, reported the isolation of a thermophilic bacterium G. thermoleovorans that degraded C13–C20 n-alkanes within the temperature range of 55–65 °C. Interestingly, this bacterium could not grow in the presence of n-alkanes with <C9 and >C20 [29].
Two strains of G. stearothermophilus (KTCC-B2M and KTCC-B7S) were isolated by enrichment of oil-contaminated samples from Kuwait, in the presence of crude oil. The two strains could degrade C15–C18 n-alkanes, while n-alkanes with lower carbon numbers, alkenes and aromatics were less efficiently degraded [30] (ST7). In another study, two anaerobic facultative strains, G. thermoleovorans B23 and H41, were isolated from produced water from deep petroleum reservoirs in Japan. These strains could degrade C13–C26 n-alkanes within a temperature range of 50–80 °C. However, less degradation was observed when shorter alkane chains (<C12) were used [31] (ST8). Further investigations demonstrated that these strains could degrade n-alkanes through terminal oxidation, followed by β-oxidation [31].
A strain NG80-2 of G. thermodenitrificans was isolated following an enrichment culture of a sample from a deep oil reservoir in China, in the presence of crude oil, at 73 °C. This strain could degrade C15–C36 n-alkanes, at an optimum temperature of 65 °C. However, no growth was observed with short-chain n-alkanes (C8–C14), and those >C40. Interestingly, as will be discussed in Section 3.3.1, alkB, a gene encoding the enzyme system responsible for the first step of the degradation of n-alkanes, was not found in this strain [32] (ST9); instead, a novel alkane monooxygenase, LadA, was identified [33]. In a different study, various species of Geobacillus (G. thermoleovorans strain 27, Geobacillus caldoxylosilyticus 17, Geobacillus pallidus 2, Geobacillus toebii 1, Geobacillus sp. 3) that degrade n-hexadecane have been isolated following an enrichment of contaminated soil samples. In that study, evidence indicated that the expression of alkB gene was induced by n-hexadecane [34] (ST10). The use of the same substrate, n-hexadecane, in the enrichment of a medium containing an inoculum from Dongxin oil reservoir, China, led to the isolation of a thermophilic bacterium TH2, that was able to degrade petroleum hydrocarbons, mainly n-alkanes, at an optimum temperature of 70 °C [35] (ST11). In that study, species identification was not carried out.
Recently, Tourova et al. reported three strains, G. toebii B-1024, Geobacillus sp. 1017 and Aeribacillus pallidus 8m3, capable of degrading C10–C30 n-alkanes [36] (ST12). The alkB gene, encoding rubredoxin-dependent alkane monooxygenase, was found in these three strains, while another gene, ladA gene, which codes for flavin-dependent alkane monooxygenase, was only present in the strains B-1024 and 1017 [36]. That study revealed (as will be discussed in Section 3.3.1), for the first time, the simultaneous presence of alkB and ladA genes, responsible for oxidation of medium-chain and long-chain n-alkanes [36]. In addition, the genome of Geobacillus sp. 1017 was sequenced, and several key genes associated with the degradation of petroleum hydrocarbons have been identified [37].
In the aforementioned studies, bacteria were isolated following enrichment in the presence of hydrocarbons. However, in 2006, hydrocarbon degrading bacteria were isolated using an alkJ probe, an alkane hydroxylase gene involved in the degradation of n-alkanes [38] (ST13). Bacteria in volcanic samples from Greece were first cultured in rich media in a temperature range of 60–80 °C, and around 150 strains were isolated. Thereafter, genetic investigations using the alkJ probe led to the identification of 10 strains, and four were G. thermoleovorans, three G. stearothermophilus, two Geobacillus anatolicus and one Bacillus aeolius [38]. Further studies confirmed that these strains could utilize long-chain n-alkane compounds from crude oil [38]. As will be discussed in Section 4.1.2, the use of genetic approaches provides an alternative to the selection and isolation of PHDT.

2.2. Monocyclic Aromatic Hydrocarbons (MAHs)

The first evidence of MAH biodegradation was provided in 1974. A strain of G. stearothermophilus PH24 was selected from industrial sediment and grew at 55 °C in the presence of phenol. Further studies indicated that, in addition to phenol, this strain could also degrade catechol, at an optimum temperature of 50 °C [39] (ST14). Evidence was also provided that this strain could cleave catechol at the meta-position. A year later, the same strain was shown to degrade other MAHs including o-, m- and p-cresol, 3-methylcatechol and 4-methylcatechol [40,41] (ST15).
Using soil that had been pasteurized for 10 min at 80 °C, Adams and Ribbons isolated a strain of G. stearothermophilus IC3 by enrichment in the presence of phenol and m-cresol. In addition to m-cresol and phenol, this strain could also utilize benzoic acid, and the degradation of phenol led to catechol, followed by cleavage at the meta-position [42] (ST16). A year later, another phenol-degrading G. stearothermophilus strain BR219 was isolated (by enrichment in the presence of phenol), using river sediment from the USA (ST17) [43]. Further investigation led to the characterization of phenol hydroxylase, the first enzyme involved in phenol degradation [44].
Dong et al. (1992) reported the isolation of a G. stearothermophilus strain FDTP-3; however, detailed information was not available on the source of the inoculum and the conditions of the isolation [45] (ST18). The strain could degrade phenol along with catechol, but not benzoic acid. Two genes of the meta pathway of phenol degradation, phenol hydroxylase and catechol 2,3-dioxygenase, were also characterized by cloning in Escherichia coli [45,46]. Using phenol as the sole substrate, a Geobacillus sp. A2 strain was isolated from a hot spring sample (from Iceland), in an enrichment experiment at 70 °C [46,47] (ST19). This strain could grow efficiently in the presence of phenol or isomers of cresol (o-, m-, and p-cresol) at 70 °C. In addition, the cleavage of the aromatic ring proceeds through the meta-position. Genetic analysis showed that this A2 strain was closely related to G. thermoleovorans [46,47]. In a different study, two strains, Thermus aquaticus ATCC 25,104 and Thermus sp. ATCC 27978, were shown to degrade the MAHs benzene, toluene, ethylbenzene, and xylene, at 70 °C (for strain ATCC 25104) and at 60 °C (for ATCC 27978), respectively [48] (ST20). In addition, using I4C-labeled benzene and toluene, radioactive 14CO2 was identified, illustrating the complete mineralization of these compounds [48]. In another study, three strains of phenol degrading Bacillus were isolated following an enrichment of contaminated samples in the presence of phenol, at 50 °C [49] (ST21). These strains could degrade phenol (along with o-, m-, and p-cresol) in an optimum temperature range of 50–55 °C, and this degradation (for phenol) is carried out via the meta-pathway. The presence of catechol 2,3-dioxygenase was also detected in this study [49].
A halotolerant and thermophilic strain, Aeribacillus pallidus VP3, was isolated following enrichment of an inoculum consisting of production water from a Tunisian oil field, in the presence of the MAH vanillic acid, at 55 °C. The selected strain VP3 could degrade several MAH compounds including benzoic, p-hydroxybenzoic, protocatechuic, gallic, p-coumaric, ferulic and caffeic acids [50] (ST22).
All of the aforementioned studies were carried out in bacteria. However, archaea have also been shown to degrade MAHs. For instance, a thermophilic strain of Sulfolobus solfataricus P2 isolated from a volcanic site, was sequenced, and its genome revealed the presence of genes encoding enzymes involved in MAH degradation, including mono- and dioxygenases [51]. This information led Izzo et al. to investigate the ability of this archaean strain to grow in the presence of MAH [52] (ST23). The results indicated that S. solfataricus P2 could actively grow on phenol as the sole source of carbon, at an optimum temperature of 80 °C, and the presence of phenol induced the expression of enzymes involved in the degradation of MAHs. As will be discussed in Section 4.1.2, this study illustrates the usefulness of the exploitation of genome information to identify potential substrates (pollutants) that a given microorganism can degrade [52].

2.3. Polycyclic Aromatic Hydrocarbons (PAHs)

In 1999, Shimura et al. provided the first evidence of the degradation of a PAH by thermophiles. The authors carried out an enrichment culture of a compost from a field in Okayama, Japan, at 60 °C using biphenyl as the sole source of carbon, leading to the isolation of Geobacillus sp JF8 strain [53] (ST24). This strain could degrade biphenyl, naphthalene, and the MAH benzoic and salicylic acids. Polychlorinated biphenyl congeners including tetra- and penta-chlorobiphenyl could also be degraded. However, no degradation was observed with the aromatic compounds PHEN, ANTH, benzene, o-xylene, m-xylene, p-xylene and toluene [53].
The ability of two thermophilic strains, Bacillus subtilis BUM and Mycobacterium vanbaalenii BU42, degrading PHEN were tested in degrading BZP at 55 °C. Neither of the strains could degrade BZP when used as the sole source of carbon [54]. However, when BZP was used as co-substrate with PHEN, the strain BUM could degrade BZP, while the strain BU42 could not [54] (ST25). This investigation illustrated the usefulness of cometabolism (in the case of the BUM strain) in the removal of recalcitrant PAH, as will be discussed in Section 4.2.2.
Another example of HMW-PAH degradation by cometabolism was provided by Feitkenhauer and Markl [55] (ST26). Using a mixture of thermophilic bacteria Bacillus sp. and Thermus sp. pre-isolated from hot springs, compost piles and industrial wastewater, the authors showed that these two strains could degrade the PAHs acenaphthene, FLT, PYR and BZP, but only in the presence of hexadecane [55] (ST26). In this context of cometabolism, hexadecane was the growth substrate, while PAHs were non-essential substrates, as will be discussed in Section 4.2.2.
Using soil samples from contaminated areas in Kuwait as inoculum, several thermophilic bacteria were isolated by enrichment in the presence of a mixture of the following PAHs (NAPH, PHEN, ANTH, PYR and FLR) and the heterocyclic polyaromatics (benzothiophene, dibenzothiophene dibenzofuran and carbazole), at 60 °C. Several thermophilic bacteria were isolated, including Bacillus firmus, Bacillus pallidus, Anoxybacillus sp., Paenibacillus sp., and Saccharococcus sp. [56] (ST27). Interestingly, when glucose was used as substrate, the thermophilic bacteria growth was inhibited, and this inhibition was explained by catabolite repression, in which glucose acts as a repressor for the synthesis of some enzymes involved in the biodegradation of PAHs [56]. The existence of this inhibitory effect in the context of cometabolism during degradation of petroleum hydrocarbons has already been reported [57], and as will be discussed in Section 4.2.2, this inhibition puts a caveat on the process of cometabolism.
Three thermophilic Geobacillus sp. strains have been isolated following an enrichment of compost in the presence of PHEN at 60 °C. Further studies have shown that these strains could also degrade the PAHs FLR and FLT [58] (ST28). A thermophilic bacterium, Nocardia otitidiscaviarum TSH1, was isolated following an enrichment of petro-industrial wastewater soil from Iran, in the presence of NAPH. The strain could degrade NAPH at 60 °C, and further analyses have led to the identification of key NAPH metabolites [59,60] (ST29). As will be discussed in Section 3.3.3, overall, the NAPH degradation pathway differs from that of mesophilic bacteria. The same strain has also been shown to degrade PHEN and ANTH, and several of their metabolites have been identified [60] (Section 3.3.3). In another study, an NAPH degrading thermophilic bacterium, Geobacillus sp. G27, was isolated from a geothermal oil field in Lithuania. The strain could degrade NAPH, along with ANTH, protocatechuic acid, benzene-1,3-diol, phenol and benzene at an optimum temperature of 60 °C. However, no growth was observed with catechol [61] (ST30). Chromatographic analyses have indicated that the first NAPH ring opening occurred via ortho-cleavage, leading to protocatechuic acid, as will be discussed in Section 3.3.3.
In 2000, the enrichment of a hydrocarbon contaminated soil from Germany in the presence of NAPH at 60 °C led to the isolation of a G. thermoleovorans Hamburg 2 strain [62] (ST31). Further studies showed the presence of some NAPH metabolites generated by this strain, such as benzoic acid, which has not been commonly reported in mesophiles (Section 3.3.3).
Two strains of Geobacillus genus, G. pallidus XS2 and XS3, isolated from oil contaminated soil from China, were reported to degrade PHEN and FLR. The strains were selected following enrichment in the presence of PHEN, FLR and crude oil, at 60 °C. Further analyses showed that more than 70% of the tested PAHs were degraded within 20 days (from 250 mg/L), and, in addition, the strain could degrade n-alkanes and produce bioemulsifier [63] (ST32). In another study, the enrichment of a soil sample from a Chinese oilfield, in the presence of the crude oil at 70 °C, led to the isolation of Geobacillus sp. SH-1. This strain could degrade NAPH and C12–C23 n-alkanes [64] (ST33). Several metabolites of NAPH have been identified (Section 3.3.3).
Recently, G. stearothermophilus strain A-2 was isolated from produced water from Dagang petroleum reservoir, China, at a temperature of 73 °C. Further studies showed that this strain could degrade NAPH, methylated PHEN, FLR, benzo[b]fluorenes and long-chain alkanes (nC22–nC33). However, shorter chains (nC14–nC21) were not efficiently degraded. In addition, this strain had a strong surface hydrophobicity and produced a bioemulsifier, making it an ideal strain for bioremediation [65] (ST34). In another recent study, a strain of Bacillus licheniformis M2-7 was isolated from an inoculum from a hot spring in Mexico, and was proved to degrade BZP. Further studies have led to the identification of the MAH phthalic acid as a BZP metabolite, and the characterization of the catechol dioxygenase enzyme, a clear illustration of the ability of this strain to convert BZP to aliphatic derivatives [66] (ST35).

3. General Findings

3.1. Geobacillus Are the Most Dominant PHDT

As Table 1 shows, a total of 49 different strains of thermophilic bacteria and one archaea (S. solfataricus) degrading hydrocarbons were isolated and/or characterized, and they all fall in the following 12 genera (Figure 2): Thermomicrobium, Sulfolobus, Anoxybacillus, Paenibacillus, Saccharococcus, Mycobacterium and Nocardia at 1.7% each, Aeribacillus (3%), Thermoleophilum (5%), and Bacillus (10%); the most striking observation was that the bulk of strains, around 63%, belong to the genus Geobacillus.
It is even more surprising that these strains were selected in different parts of the world (encompassing the five continents and around 20 different countries) and from different sources including hot springs, produced water, soil samples, geothermal oil fields, and compost samples, among others. Thermophiles are known to be distributed in more than 70 genera, with a total of 140 species [35]. Although only seven genera of these thermophiles have been reported in this review, however, the skew of species to Geobacillus genus deserves further discussion.
In comparison, the distribution of the mesophiles that degrade hydrocarbons is wider. More than 150 different genera have been reported, and a careful observation of the seminal reviews on this topic clearly shows that not a single genus represents even more than 25% of the reported species [4,5,7,67]. This begs the question, why do thermophiles degrading hydrocarbons belong predominantly to the genus Geobacillus?
Bacteria of Geobacillus genus are endospore-forming microbes, and are aerobic or facultative anaerobic. They are primarily thermophiles, thus are found to proliferate in environments where temperatures are relatively high. However, surprisingly, they have even been found in a wide range of moderate and low-temperature environments, including the deep sea, which are below their minimum requirement for growth [17,68]. Evidence indicates that their endospores resist high stress conditions, including temperature variation, UV light and desiccation [68]. These bacteria are also characterized by their lower size of spores (around 1 µm diameter), allowing them to remain suspended in the atmosphere for long periods, time that can be sufficient for their widespread distribution to all continents [17,68]. In addition, genomic investigations show that these microbes have a battery of gene encoding transporters and hydrolytic enzymes, specifically those adapted to using plant biomass as a source of carbon and energy [17,68,69,70]. These observations, coupled with the high reproduction rate of Geobacillus, have been proffered to be the driving factors for the ubiquitousness of this thermophilic genus on earth [17,68,69,70]. However, their high presence in environments contaminated with petroleum products underscores that these bacteria are also endowed with gene encoding enzymes involved in the degradation of both aliphatic and aromatic products [17], and as discussed in Section 3.3, some of these catabolic genes (or enzymes) have been characterized.
It is also worth noting that, among the Geobacillus genera, G. stearothermophilus and G. thermoleovorans represent 67% of all Geobacillus species. G. stearothermophilus is the most prominent Geobacillus species (30%), and is also the reference species in this genus [22]. Finally, almost 81% of the reported thermophiles (Table 1) belong to the family Bacillaceae.

3.2. Short Chain n-Alkanes Are Less Efficiency Degraded

Most studies in which several n-alkanes were investigated showed clearly that, in general, shorter chain n-alkanes (generally <C9) were less efficiently degraded than longer chains (>C25) [3,4,5,6,7,8,9] (ST2).
These observations are in line with those made on mesophiles, that midsized n-alkanes (C10–C20) are generally more amenable to degradation than shorter or longer chain n-alkanes. One of the reasons for the slow utilization of longer chains is their low water solubility, thus their low bioavailability compared to midsized n-alkanes [71]. On the other hand, although short-chain n-alkanes have higher aqueous solubility, they are toxic to microbes or cells, and the mechanisms of this toxicity are associated with their uptake and dissolution in the cell membrane [71]. Thus, though short chain n-alkanes are more bioavailable than midsized or long chain n-alkanes, they are associated with cell toxicity, hence their inhibition of microbial growth.

3.3. Specific Observations in Relation to Mechanisms of Degradation

3.3.1. n-Alkanes

In general, the mechanisms of n-alkane degradation proceed in a similar fashion with both mesophiles and thermophiles. This process is initiated by the action of monooxygenases, enzymes that incorporate one atom of O2 into the n-alkane chain, or by dioxygenases, enzymes that can incorporate two O2 atoms. Both pathways will lead to the generation of alcohol, which will then be converted into fatty acids, and the latter will then be subjected to β-oxidation to generate energy and CO2 through the central metabolism of the bacteria [72]. The genes involved in this pathway have been studied in mesophiles, specifically in the Pseudomonas genus. The n-alkane pathway is controlled by two operons: The alkBFGHJKL operon encodes components of the alkB system, the membrane-bound n-alkane hydroxylase system, and the alkT/alkS operon. The latter, lkT/alkS operon, encodes rubredoxin reductase enzyme, which also regulates the expression of the alkBFGHJKL operon [73,74]. This AlkB gene has also been characterized in thermophilic bacteria Geobacillus and Aeribacillus [34,36] (ST10, 12). Thus, it is likely that other genes belonging to these operons are present in thermophiles that degrade petroleum products. For instance, as will be discussed in Section 4.1.2, the alkJ gene from Pseudomonas has been used to detect and isolate n-alkanes utilizing thermophilic strains of G. thermoleovorans, G. stearothermophilus, G. anatolicus and B. aeolius (ST13).
In addition to the AlkB system, two other oxygenases have been reported in mesophiles. These are cytochrome P450 monooxygenases, which belong to the cytochrome CYP153 family [75], and flavin-dependent dioxygenases, encoded by the almA gene [76]. AlkB and CYP153 enzymes are generally implicated in the oxidation of medium-chain C5–C17 n-alkanes while the almA system is associated with the oxidation of long-chain C10–C30 n-alkanes [73,75,76].
Interestingly, so far, the two systems, CYP153 and AlmA, have not been reported in thermophilic bacteria degrading n-alkanes. Instead, these thermophiles express a novel type of alkane monooxygenase system, LadA, which was initially discovered in a G. thermodenitrificans NG80-2 strain [32,33] (ST9). Similar to AlmA, LadA is a flavin-dependent monooxygenase of the family of bacterial luciferases, and is responsible for the degradation of long-chain n-alkanes C15–C36. Recently, the simultaneous presence of alkB and ladA genes has been reported in two strains of Geobacillus and one of Aeribacillus [36] ([ST12).
In summary, so far, research has shown that n-alkane degrading mesophiles and thermophiles express AlkB monooxygenase enzymes; however, in addition, these mesophiles have CYP153 and AlmA enzymes, while these thermophiles express LadA monooxygenases.

3.3.2. MAHs

Phenol has been the most studied MAH in thermophiles. As in mesophiles, the biodegradation of this compound is initiated by hydroxylation to generate catechol, a reaction mediated by phenol hydroxylase. In mesophiles, this catechol is metabolized either through ortho- or meta-cleavage (Figure 3), in the presence of catechol 2,3-dioxygenase enzyme. However, detailed observation of the data published for thermophiles has shown that, so far, only meta-cleavage is present in these thermophiles (ST14, 16, 19, 21) (Figure 3). The two enzymes, phenol hydroxylase and catechol 2,3-dioxygenase, have been characterized in G. stearothermophilus (ST17, 18) and Bacillus sp. (ST21). Using a G. stearothermophilus strain, Omokoko et al. reported the complete sequence of an operon encoding the meta-pathway genes, and characterized the first transcriptional regulator of phenol metabolism [77]. In summary, thermophiles differ from mesophiles in their preference for the meta-cleavage of MAHs.

3.3.3. PAHs

The mechanism of PAH degradation in thermophiles has received little attention. So far, in total, only five reports have studied this. Four have been dedicated to NAPH (ST29, 30, 31, 33), and in one of them (ST29), the analyses were extended to PHEN and ANTH; and one study investigated the biotransformation of BZB (ST35).
Like mesophiles, thermophiles initiate the degradation of PAHs (in this case, NAPH, PHEN and ANTH) by the action of mono- or dioxygenases, enzymes that mediate the formation of mono- and then di-hydroxylated aromatic compounds. As Figure 4, Figure 5 and Figure 6 show, the mono- and di-hydroxylated derivatives of NAPH, PHEN and ANTH have been identified (ST29, 30, 31, 33). Thereafter, these di-hydroxy-aromatic compounds are subjected to cleavage, either between the hydroxyl groups (meta-cleavage) or proximal to one of the two hydroxyl groups (ortho-cleavage), to generate an aromatic compound with less than one ring. A careful observation of NAPH degradation (Figure 4) indicates that cleavage of the first ring occurs at the meta-position. For instance, the derivatives 3-(2-hydroxyphenyl)-propanoic acid (ST31), 2-hydroxycinnamic acid (ST29), 4-(2-hydroxyphenyl)-2-oxo-but-3-enoic acid (ST31, 33) and (2E)-3-(2-hydroxyphenyl)prop-2-enal (ST33) are likely to result from the ring opening of 1,2-dihydroxynaphthalene at the meta-position. Likewise, the existence of 3-(2-carboxyphenyl)-2-propenoic acid (2-carboxycinnamic acid) (ST29, 31) could result from the meta-cleavage of 2,3-dihydroxynaphthalene (Figure 4).
Study ST30 identified protocatechuic acid, and, so far, the existence of this metabolite has not yet been identified in mesophiles, although it has been proposed [78].
Three studies (ST29, 31, 33) out of four on NAPH degradation showed the presence of a benzoic acid metabolite. To the best of the knowledge of the author, in only one study has this compound been reported in the context of NAPH degradation in mesophiles [79]. This metabolite has also been reported in the context of ANTH degradation (in mesophiles), and was proposed to be derived from decarboxylation of phthalic acid [80]. Interestingly, phthalic acid has been reported in thermophiles (ST30, 31). Thus, the fact that most studies on NAPH degradation have identified benzoic acid highlights the preference of thermophiles for this reaction (decarboxylation of phthalic acid). This benzoic acid was also reported in the degradation of ANTH by the thermophilic strain N. otitidiscaviarum TSH1 (Figure 5, ST29).
In relation to PHEN (Figure 5), the metabolite 4-[1-hydroxy(2-naphthyl)]-2-oxobut-3-enoic acid could be derived from the first ring opening, at the meta-position, of 3,4-dihydroxyphenanthrene, while 2,2-biphenic acid could result from ortho-cleavage of 9,10-dihydroxyphenanthrene. However, the observation of ANTH degradation (Figure 6) indicates that the first ring opening is the result of the ortho-cleavage of 1,2-dihydroxy-anthracene (which is probably derived from 1,2-dihydroxy-1,2-dihydroanthracene) to generate 3-(2-carboxyvinyl) naphthalene-2-carboxylic acid, another identified metabolite (Figure 6, ST29).
In summary, in thermophiles, the first ring opening of NAPH occurs at the meta-position only, as is the case with phenol, and that of ANTH proceeds through ortho-cleavage, while both meta- and ortho-cleavage exist in PHEN degradation.

4. Gaps and Strategies to Improve Biodegradation by Thermophiles

4.1. Isolation of More Thermophilic Microbes

4.1.1. Use of Experimental Set-Up

Table 1 shows that most strains were active within the range 40–80 °C, with optimum temperatures of <70 °C (most of them being around 60 °C). Only one strain, S. solfataricus P2, an archaea, had an optimum temperature >70 °C (optimum temperature being 80 °C) (ST23). As discussed in Section 1, these microbes fall into the group of moderate- and extreme-thermophiles. Thus, so far, no hyper-thermophiles (whose optimum temperatures are >85 °C) that degrade petroleum hydrocarbons have been described. The following reasons could explain the absence of the hyper-thermophiles.
First, most conditions of isolation of these thermophilic strains had involved the enrichment technique. In this approach, inocula or samples that are presumed to contain microbes are incubated at a fixed temperature, in the presence of the substrate of interest, and most of these enrichment experiments were carried out at temperatures <70 °C; in fact, 55 and 60 °C were the most commonly used temperatures. During the enrichment process, microbes that are favored are those whose growth is optimum at temperatures set in the experiments. Therefore, it is not surprising that most strains had optimum temperatures <70 °C and no hyper-thermophiles were selected during these experiments.
Thus, it is reasonable to argue that, so far, researchers have deliberately focused their work on moderate- and extreme-thermophiles. Working on hyper-thermophiles requires maintaining temperature of 85–120 °C throughout the experimental conditions, which can be a limitation in the context of routine cultures. This may explain why most enrichment experiments were carried out at temperatures <70 °C, even when samples that are likely to contain hyper-thermophiles were used as inocula (for instance, samples isolated near a volcanic site, in ST13). Thus, the degradation of petroleum hydrocarbons by hyper-thermophiles awaits exploration.

4.1.2. Genomic Approach

Genetic approaches can also be used to isolate pollutant-degrading microbes in general, or PHDT in particular. This strategy employs molecular probes that are based on the sequences of key genes encoding catabolic enzymes in the pollutant degradation pathway. The advantage of this approach is that the probe can be tailored to target a specific enzyme, thus a specific pathway, therefore excluding microbes that do not have that particular pathway. As discussed in Section 3.3.1, this approach has been used to isolate PHDT. For instance, using alkJ gene encoding alkane hydroxylases from Pseudomonas putida and Pseudomonas oleovorans (two mesophiles), Meintanis et al. screened a pool of 150 thermophilic bacteria isolated from a volcanic site for the presence of alkJ gene. A total of 10 thermophilic strains (nine Geobacillus and one Bacillus genera) were identified, and further studies proved that they could all degrade long chain n-alkanes and crude oil [38] (ST13).
In mesophiles, this approach has also been used to identify bacteria that degrade the recalcitrant PAHs (especially pyrene) by using a probe based on the sequence of the aromatic ring hydroxylating dioxygenase enzyme (ARHDO), responsible for initial dihydroxylation [81,82,83]. As shown in Table 1, only a few thermophiles that degrade complex PAH compounds have been isolated so far. Thus, for instance, the use of ARHDO probes (based on sequences from mesophiles) could provide an opportunity to isolate and characterize more thermophiles that degrade PAHs.
PHDT can also be identified by exploiting the available information on the genome sequences of thermophiles. Specific genes encoding enzymes involved in petroleum hydrocarbon (or any pollutant) degradation can be sought in the genome sequences, and if these genes are present, in vitro studies can be initiated to assess the thermophile’s ability to grow in the presence of these pollutants. As discussed in Section 2.2 (ST23), this approach has successfully been tested, and led to the identification of the archaean strain S. solfataricus P2 as a MAH degrader. Thus, more PHDT can be identified by employing this strategy, since the genomes of a sizable number of thermophilic strains have now been sequenced (https://www.ncbi.nlm.nih.gov/genome). New “omics” techniques, such as functional metagenomic, can allow the identification of genes (thus their protein functions) present in genomes of microbial communities without the need of prior in vitro culture [84,85,86]. Using this approach, genes involved in the degradation of various pollutants, including PAHs, have been identified, cloned and functionally expressed [87]. Thus, the exploitation of this approach in the context of hydrocarbon degradation by thermopiles will immensely contribute in discovering novel and unique genes, which can then be used to isolate novel PHDT strains.

4.2. Improving Biodegradation

Several strategies to improve biodegradation of the pollutants have been evaluated in the context of mesophiles. The same strategies can be applied and can add value to the degradation of petroleum hydrocarbons by thermophiles. These strategies are summarized next.

4.2.1. Bioaugmentation

This strategy is based on the addition of preselected and active microorganisms to a microbial community to enhance the ability of this microbial community to degrade pollutants. In the context of the use of mesophiles, this approach has been employed in the biodegradation of various recalcitrant and complex pollutants [88,89,90,91]. As Table 1 shows, many single thermophilic strains have been isolated and characterized as degraders of petroleum hydrocarbons. Thus, each of these strains could potentially be used in the context of bioaugmentation. However, so far, not a single bioaugmentation experiment has been reported using thermophiles for the degradation of petroleum hydrocarbons.

4.2.2. Cometabolism

Cometabolism has been widely used in mesophilic conditions to increase pollutant degradation [5]. This approach uses two different substrates (pollutants), a non-growth and a growth-promoting substrate. As the name indicates, the non-growth substrate alone does not efficiently support microbial growth, leading to slow growth. However, when the growth-promoting substrate is added, microbial growth will increase, and this, in turn, will lead to degradation of the non-growth substrate [5].
Interestingly, three studies have evaluated this concept of cometabolism in the context of thermophiles (ST25, 26, 27). In the first (ST25), the strain B. subtilis BUM could not degrade the recalcitrant BZP when used as sole substrate. However, the addition of PHEN, a growth-promoting substrate, led to the degradation of BZP, a classic illustration of cometabolism. Likewise, in ST26, the degradation of the PAH compounds acenaphthene and FLT, PYR and BZP by a mixture of strains of Geobacillus sp. and Thermus sp. were augmented when the n-alkane hexadecane was used as a growth-promoting substrate.
However, as has been shown in mesophilic conditions, the use of growth-promoting substrates can inhibit the degradation of the non-growth substrate [92,93]. For instance, in mesophilic conditions, one study showed that the use of glucose (as growth-promoting substrate) inhibited the expression of enzymes involved in PAH biodegradation [93]. ST27 confirms this limitation in the context of thermophiles. Indeed, the use of glucose led to the inhibition of degradation of the PAHs NAPH, PHEN, ANTH, PYR and FLR, and the heterocyclic compounds benzothiophene, dibenzothiophene, dibenzofuran and carbazole. Thus, as discussed in a recent review, a prior assessment of the effects of growth-promoting substrates should be investigated before combining non-growth and growth-promoting substrates in a culture [2]. However, evidence indicates that the inhibition of degradation in the context of cometabolism generally occurs when a single bacterial strain is used as inoculum [2]. Thus, inoculum consisting of a mixture of bacterial strains should be considered so as to minimize the possible negative effect of growth-promoting substrates, a strategy supporting the use of bioaugmentation as discussed in Section 4.2.1.

5. Concluding Remarks

It is well established that increasing temperature leads to better degradation of pollutants. Thus, the use of thermophilic microbes provides an effective strategy to improve the overall biodegradation process. Yet, so far, most of the work on biodegradation has been carried out using mesophiles, and little is known about thermophiles.
This literature review has highlighted important findings on PHDT. For instance, more than 60% of PHDT strains isolated so far belong to one single genus, Geobacillus, while the family Bacillaceae represents more than 80% of strains. Some PHDT express a specific n-alkane hydroxylase gene, LadA, which has not been reported in mesophiles so far. In the degradation of monoaromatic compounds, evidence indicates that PHDT preferentially uses meta-cleavage pathways, yet both ortho- and meta-pathways exist in mesophiles. All of these observations show that thermophiles in general and PHDT in particular are endowed with unique biochemical pathways, and this is in line with the fact that thermophiles have been used as a source of enzymes or molecules in industrial biotechnology.
This review has also highlighted important gaps in our understating of PHDT. For instance, up to the present time, all PHDT described so far belong to the group of moderate- and extreme-thermophiles, and not a single hyper-thermophile has been reported so far. However, the exploitation of the genome information and the functional metagenomic (which bypasses the initial limitation of the in vitro culture) provide a great opportunity to discover and isolate novel PHDT strains (including hyper-thermophile).
Various strategies, such as bioaugmentation, cometabolism, and the new emerging genetically modified microorganisms and nanotechnology [94,95,96,97], have been tested to improve the efficiency of pollutant degradation by mesophiles, and some of these strategies have been used in real conditions of bioremediation. Thus, using the vast amount of information on mesophiles, experiments can now be scaled up for the bioremediation of contaminated samples by thermophiles.

Funding

This research was funded by the Deanship of Scientific Research (DSR) of the King Fahd University of Petroleum and Minerals (KFUPM), grant number IN171022. AN is supported by KFUPM.

Conflicts of Interest

The author declares no conflict of interest

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Figure 1. Various advantages of carrying out biodegradation reactions at higher temperatures (T°), thus, in the presence of thermophiles.
Figure 1. Various advantages of carrying out biodegradation reactions at higher temperatures (T°), thus, in the presence of thermophiles.
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Figure 2. Distribution of genera of the 59 thermophilic strains degrading petroleum hydrocarbons summarized in Table 1.
Figure 2. Distribution of genera of the 59 thermophilic strains degrading petroleum hydrocarbons summarized in Table 1.
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Figure 3. Pathways of phenol degradation. So far, only the meta-cleavage pathway has been described in thermophilic bacteria, yet both pathways (meta- and ortho-) are present in mesophilic bacteria.
Figure 3. Pathways of phenol degradation. So far, only the meta-cleavage pathway has been described in thermophilic bacteria, yet both pathways (meta- and ortho-) are present in mesophilic bacteria.
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Figure 4. Possible pathways of naphthalene (NAPH) degradation by thermophilic microbes.
Figure 4. Possible pathways of naphthalene (NAPH) degradation by thermophilic microbes.
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Figure 5. Biodegration pathways of phenanthrene in thermophilic bacteria. All these metabolites were identified from a strain of Nocardia otitidiscaviarum bacterium (ST29).
Figure 5. Biodegration pathways of phenanthrene in thermophilic bacteria. All these metabolites were identified from a strain of Nocardia otitidiscaviarum bacterium (ST29).
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Figure 6. Biodegration pathways of anthracene in thermophilic bacteria. All these metabolites were identified from a strain of Nocardia otitidiscaviarum bacterium (ST29).
Figure 6. Biodegration pathways of anthracene in thermophilic bacteria. All these metabolites were identified from a strain of Nocardia otitidiscaviarum bacterium (ST29).
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Table
Table 1. Summary of studies on biodegradation of petroleum products by thermophilic microbes.
Table 1. Summary of studies on biodegradation of petroleum products by thermophilic microbes.
StudySource of SamplesConditions of IsolationThermophiles Used or IsolatedRange of Temperatures and/or Optimum Temperature (in Parentheses)Petroleum Products Degraded or Other Characteristics of the StrainsReference
n-alkanesST1Samples of water or soil Enrichment culture in the presence hydrocarbon at 57 °CBacillus stearothermophilus(57 °C)Hydrocarbons[23]
ST2Mud sample from a littoral area of North Carolina, USAEnrichment culture in the presence
n-hexadecane at 50 °C		Thermomicrobium fosteri PTA-1,42 to 70 °C
(60 °C)		* n-alkanes (nC10–C20), 1-Alkenes (C14–C1), alcohol (C12–C17) and ketones (C14–C17) C14 * but not <C9 (n-alkanes), <C9 (alkene and ketones) and <C11 (alcohol)[24]
ST3Mud samples from Yellowstone National Park, USAEnrichment culture in the presence n-heptadecane at 60 °CThermoleophilum minutum YS-3
Thermoleophilum album YS-4		(60 °C)* n-alkane C10–C20 but not those <C9
* C6–C8 alkenes, but not <C6 alkenes		[25,26]
ST4Hot water springs from Kunashir Island, RussiaNot providedGeobacillus stearothermophilus 16
Thermus ruber 12		55–73 °C
(60 °C)		Paraffins[27]
ST5Mud and water from various parts in the USAEnrichment in the presence of
n-heptadecane at 60 °C		T. album45 to 70° C
(60 °C)		C13–C30[28]
ST6Mud and water from the USAEnrichment in the presence of n-heptadecane at 60 °CGeobacillus thermoleovorans55–65 °Cn-alkane C13–C20 but not those <C9 or >C20[29]
ST7Contaminated samples from KuwaitEnrichment in the presence of crude oil at 60 °CG. stearothermophilus strains KTCC-B7S and KTCC-B2M60 °CC15–C18 n-alkanes but not of lower carbon numbers, alkenes and aromatics[30]
ST8Production water samples from deep petroleum reservoirs, JapanNo enrichmentG. thermoleovorans
B23 and H41		50–80 °C
(70 °C for B23)
(65 °C for H41)		* C13–C26 n-alkanes but not for
C9–C12
* Evidence of terminal oxidation of n-alkanes		[31]
ST9Deep subterranean oil reservoir, ChinaEnrichment in the presence of crude oil at 73 °CGeobacillus thermodenitrificans NG80-245–73 °C
(65 °C)		* C15–C36 n-alkanes but not the short-chain (C8–C14) and those longer than C40
* No alkB gene homologs found in NG80-2		[32]
ST10Soil samples, IrelandEnrichment at 60 °C in the presence of n-hexadecaneG. thermoleovorans 27,
Geobacillus caldoxylosilyticus 17,
Geobacillus pallidus 2,
Geobacillus toebii 1,
Geobacillus sp. 		(60 °C)* n-hexadecane
* Expression of AlkB gene induced by n-hexadecane		[34]
ST11Samples from wells in the Dongxin reservoir (temperature 60–80 °C), ChinaEnriched culture in the presence of n-hexadecaneThermophilic strain TH-240–85 °C
(70 °C)		Degradation of the petroleum hydrocarbons, mainly n-alkanes[35]
ST12Formation water from the Dagang oilfield and from a thermal spring in the Baikal rift zone, Russia Not explainedG. toebii B-1024,
Geobacillus sp. 1017,
Aeribacillus pallidus 8m3		38–70 °C
(60 °C)		* C10–C30 n-alkanes (for B-1024)
* C13–C19 n-alkanes (for 1017)
* C11–C29 n-alkanes (for 8m3)
* Simultaneous presence of alkB and ladA genes involved in the degradation of alkanes		[36]
ST13Samples from active volcano of Santorini, Greece,Bacteria were identified by alkJ probe, following culture in rich medium at 60–80 °CG. thermoleovorans (4 strains)
G. stearothermophilus (3 strains)
Geobacillus anatolicus (2 strains)
Bacillus aeolius (1 strain)		60–80 °C* Long chain alkanes of crude oil
* Use of alkJ probe to identify and select petroleum hydrocarbon degrading thermophiles (PHDT)		[38]
MonoaromaticsST14Industrial sediment, UKEnrichment in the presence of phenol, at 55 °CG. stearothermophilus PH24(50 °C)* Phenol and catechol
* Cleavage of catechol at the meta-position		[39]
ST15Same as in ST13Same as in ST13Same strain as in ST13 (G. stearothermophilus PH24) Same
as in ST13		o-cresol, m-cresol, or p-cresol,
3-methylcatechol and
4-methylcatechol		[40,41]
ST16Soil samples that had been pasteurized for 10 min at 80 °CEnrichment in the presence of m-cresol and phenolG. stearothermophilus IC3(50 °C)* Phenol, m-cresol and benzoic acid
* Phenol degradation via catechol, and cleavage via meta-position		[42]
ST17River sediment Tittabawassee, USAEnrichment in the presence of phenol, at 55 °CG. stearothermophilus BR219(55 °C)* Phenol
* Characterization of phenol hydroxylase, the first enzyme in phenol degradation		[43,44]
ST18Not providedNot providedG. stearothermophilus FDTP-360–65 °C* Phenol and catechol but no benzoic acid
* catechol 2,3-dioxygenase gene was also characterized		[45]
ST19Water and mud from a hot spring, IcelandEnrichment in the presence of phenol at 70 °CG. thermoleovorans A2(65 °C)* Phenol and o-cresol, m-cresol,
p-cresol,
* meta-cleavage of phenol		[46,47]
ST20Not providedNot providedThermus aquaticus ATCC25104
Thermus sp. ATCC 27978		(70 °C)
(60 °C)		* Degradation of BTEX (benzene, toluene, ethylbenzene, and xylenes)
* I4C-labeled benzene and toluene were metabolized to 14CO2		[48]
ST21Samples from a waste treatment plant (UK) and a phenol-contaminated industrial effluent (lake Zealand)Enrichment in presence of phenol at 50 °CBacillus sp.50–55 °C* Phenol degradation
* Detection of catechol
2,3-dioxygenase
* Cleavage of aromatic via the meta-pathway
* Catechol 2,3-dioxygenase was inactivated at high oxygen		[49]
ST22Production water from an oil field, TunisiaEnrichment in the presence of vanillic acidAeribacillus pallidus, VP337–65 °C
(55 °C)		* Degradation of various aromatic compounds including benzoic, p-hydroxybenzoic, protocatechuic acids
* Strain was also halotolerant (can grow in the presence of 100 g L−1 of NaCl)		[50]
ST23Volcanic site at Pisciarelli Solfatara, ItalyAlready preselectedSulfolobus solfataricus P2(80 °C)Anaerobic degradation of phenol[51,52]
PolyaromaticsST24Compost from a field in Okayama, JapanEnrichment culture in the presence of biphenyl as sole source of carbon, at 60 °CGeobacillus sp. JF8(60 °C)* Biphenyl, chlorobiphenyl, NAPH, benzoic
acid and salicylic acid but not PHEN, ANTH, benzene and xylene
* Related to G. Stearothermophilus		[53]
ST25PAH-contaminated soil collected from Hong KongNot providedBacillus subtilis BUM and Mycobacterium vanbaalenii BU42 (55 °C)Both strains could utilize PHEN as sole source of carbon
* None of the strains could utilize BZP as sole source of carbon
* In the presence of PHEN, only the strain BUM could degrade BZP		[54]
ST26Hot springs, compost and industrialwastewater Not providedPreselected mixture of strains of Geobacillus spp. Thermus sp.60–70 °C* Degradation of acenaphthene
FLT, PYR and BZP, in the presence of hexadecane		[55]
ST27Soil samples from contaminated areas in KowaitEnrichment in the presence of a mixture of PAHs and heterocyclic polyaromatics Bacillus firmus, Bacillus pallidus, Anoxybacillus sp., Paenibacillus sp., Saccharococcus sp.(60 °C)* Degradation of NAPH, PHEN, ANTH, PYR and FLR, benzothiophene, dibenzothiophene dibenzofuran and carbazole
* Inhibition of degradation of PAHs and heterocyclic compounds in the presence of glucose		[56]
ST28Compost sampleIncubation in the presence of PHEN at 60 °CGeobacillus sp. (3 strains)(60 °C)PHEN, FLR and FLT degradation[58]
ST29Petro-industrial wastewater soil, IranSelection in the presence of PHEN at 50 °CNocardia otitidiscaviarum TSH130–55 °C
(50 °C)		* NAPH, PHEN and ANTH degradation
* Evidence of meta-cleavage of NAPH
* Detection of various metabolites of NAPH		[59,60]
ST30Geothermal oil field in LithuaniaPre-culture in rich medium following screening in a culture in the presence of NAPHGeobacillus sp. G27 (60 °C)* Growth in the presence NAPH and ANTH, protocatechuic acid, benzene, phenol and benzene-1, 3-diol
* No growth in the presence of catechol
* Degradation of NAPH through protocatechuic acid via ortho-cleavage pathway		[61]
ST31Hydrocarbon contaminated compost, GermanyEnrichment in the presence of NAPH, at 60 °CG. thermoleovorans Hamburg 2(60 °C)* Degradation of NAPH
* Detection of phthalic and benzoic acid as NAPH metabolites		[62]
ST32Contaminated soilsamples from Yumen oilfield, ChinaEnrichment in the presence of PHEN, FLR and crude oil at 60 °CGeobacillus pallidus XS2 and XS3 (60 °C)* PHE, FLR and crude oil
* Preference of nC8–C19 for XS2 strain and n-C20–C38 for XS3 strain
* Production of emulsifier by the 2 strains		[63]
ST33Soil from a deep oil well, ChinaEnrichment in the presence of crude oil at 70 °CGeobacillus sp. SH-145–80 °C
(60 °C)		* Degradation of NAPH and
nC12–C23 (from crude oil)
* Detection of NAPH metabolites		[64]
ST34Produced water from Dagang petroleum reservoir at 73 °C, China Already growing in petroleum environmentG. stearothermophilus strain A-240–75 °C
(60 °C)		* NAPH, methylated-PHEN, FLR, benzo[b]fluorenes and long-chain alkanes (C22–C33)
* Low degradation of shorter chains (C14–C21)
* Production of bio-emulsifier		[65]
ST35Hot spring from Guerrero State, MexicoNon explainedBacillus licheniformis M2-7(50 °C)* BZP
* Identification of phthalic acid as a BZP metabolite
* Characterization of catechol dioxygenase enzyme		[66]

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Nzila, A. Current Status of the Degradation of Aliphatic and Aromatic Petroleum Hydrocarbons by Thermophilic Microbes and Future Perspectives. Int. J. Environ. Res. Public Health 2018, 15, 2782. https://doi.org/10.3390/ijerph15122782

AMA Style

Nzila A. Current Status of the Degradation of Aliphatic and Aromatic Petroleum Hydrocarbons by Thermophilic Microbes and Future Perspectives. International Journal of Environmental Research and Public Health. 2018; 15(12):2782. https://doi.org/10.3390/ijerph15122782

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Nzila, Alexis. 2018. "Current Status of the Degradation of Aliphatic and Aromatic Petroleum Hydrocarbons by Thermophilic Microbes and Future Perspectives" International Journal of Environmental Research and Public Health 15, no. 12: 2782. https://doi.org/10.3390/ijerph15122782

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