Figure 1.
Plates resulting from in vitro antimicrobial activity determination for pathogenic microorganisms, incubated for 7 days (stationary phase) at adequate temperatures. Growth positive control plates in BHI medium are also shown. From left to right, drops from 10−1 to 10−5 dilutions. Miso concentrations of 1.0 and 10.0% (w/w). Sodium chloride agar plates with the concentration of salt equivalent to each miso plate. (a) Results for preliminary test, Test I, with no nutritive media, only different miso concentrations as substrate. (b) Results for Test II, with nutritive media and different miso concentrations.
Figure 1.
Plates resulting from in vitro antimicrobial activity determination for pathogenic microorganisms, incubated for 7 days (stationary phase) at adequate temperatures. Growth positive control plates in BHI medium are also shown. From left to right, drops from 10−1 to 10−5 dilutions. Miso concentrations of 1.0 and 10.0% (w/w). Sodium chloride agar plates with the concentration of salt equivalent to each miso plate. (a) Results for preliminary test, Test I, with no nutritive media, only different miso concentrations as substrate. (b) Results for Test II, with nutritive media and different miso concentrations.
Figure 2.
Plates resulting from in vitro antimicrobial activity evaluation tests for food contaminant microorganisms (yeast and Lactiplantibacillus plantarum) after 14 days of incubation. Positive control plates of YPDF medium for yeast, and of MRS for L. plantarum. From left to right, drops from 10−1 to 10−5 dilutions. Plates with miso concentrations of 1.0 and 10.0% (w/w) or with sodium chloride at equivalent concentrations of each miso plate. (a) Results for preliminary test, Test I, with different miso concentrations or sodium chloride and no nutritive media. (b) Results for Test II, with nutritive media and different miso concentrations or equivalent concentrations of sodium chloride.
Figure 2.
Plates resulting from in vitro antimicrobial activity evaluation tests for food contaminant microorganisms (yeast and Lactiplantibacillus plantarum) after 14 days of incubation. Positive control plates of YPDF medium for yeast, and of MRS for L. plantarum. From left to right, drops from 10−1 to 10−5 dilutions. Plates with miso concentrations of 1.0 and 10.0% (w/w) or with sodium chloride at equivalent concentrations of each miso plate. (a) Results for preliminary test, Test I, with different miso concentrations or sodium chloride and no nutritive media. (b) Results for Test II, with nutritive media and different miso concentrations or equivalent concentrations of sodium chloride.
Figure 3.
Appearance of emulsions containing 5.0 to 15.0% (w/w) of miso paste and the vegan mayonnaise standard.
Figure 3.
Appearance of emulsions containing 5.0 to 15.0% (w/w) of miso paste and the vegan mayonnaise standard.
Figure 4.
Firmness (a) and adhesiveness (b) of emulsions with 5, 7.5, 10 and 15% (w/w) of miso paste and standard emulsion. Firmness (c) and adhesiveness (d) of emulsions with 7.5% (w/w) of miso paste, one of them with 0.1% (w/w) of psyllium husk. Different letters indicate significantly different results (p < 0.05) according to Tukey test or t-test.
Figure 4.
Firmness (a) and adhesiveness (b) of emulsions with 5, 7.5, 10 and 15% (w/w) of miso paste and standard emulsion. Firmness (c) and adhesiveness (d) of emulsions with 7.5% (w/w) of miso paste, one of them with 0.1% (w/w) of psyllium husk. Different letters indicate significantly different results (p < 0.05) according to Tukey test or t-test.
Figure 5.
Mechanical spectra of emulsions with 5, 7.5, 10 and 15% (w/w) of miso paste and standard emulsion. G′ corresponds to the elastic modulus and G″ corresponds to the viscous modulus.
Figure 5.
Mechanical spectra of emulsions with 5, 7.5, 10 and 15% (w/w) of miso paste and standard emulsion. G′ corresponds to the elastic modulus and G″ corresponds to the viscous modulus.
Figure 6.
Mechanical spectra of emulsions with 7.5% (w/w) of miso paste, one of them with 0.1% (w/w) of psyllium husk. G′ corresponds to the elastic modulus and G″ corresponds to the viscous modulus.
Figure 6.
Mechanical spectra of emulsions with 7.5% (w/w) of miso paste, one of them with 0.1% (w/w) of psyllium husk. G′ corresponds to the elastic modulus and G″ corresponds to the viscous modulus.
Figure 7.
Flow curves of emulsions (a) standard and with 5, 7.5, 10 and 15% (w/w) of miso paste; (b) with 7.5% (w/w) of miso paste, one of them with 0.1% (w/w) of psyllium husk. Lines represent Williamson’s model adjustment.
Figure 7.
Flow curves of emulsions (a) standard and with 5, 7.5, 10 and 15% (w/w) of miso paste; (b) with 7.5% (w/w) of miso paste, one of them with 0.1% (w/w) of psyllium husk. Lines represent Williamson’s model adjustment.
Figure 8.
Comparison of droplet size distribution of emulsions with 5, 7.5, 10 and 15% (w/w) of miso paste and standard emulsion, measured 1 (a) and 30 (b) days after emulsion preparation.
Figure 8.
Comparison of droplet size distribution of emulsions with 5, 7.5, 10 and 15% (w/w) of miso paste and standard emulsion, measured 1 (a) and 30 (b) days after emulsion preparation.
Figure 9.
Span parameter from droplet size distribution of emulsions with 5, 7.5, 10 and 15% (w/w) of miso paste and standard emulsion, measured 1 and 30 days after emulsion preparation. Different letters indicate significantly different results (p < 0.05) according to Tukey test. One asterisk and two asterisks represent non significantly (p > 0.05) and significantly (p < 0.05) different results, respectively, according to the t-Test.
Figure 9.
Span parameter from droplet size distribution of emulsions with 5, 7.5, 10 and 15% (w/w) of miso paste and standard emulsion, measured 1 and 30 days after emulsion preparation. Different letters indicate significantly different results (p < 0.05) according to Tukey test. One asterisk and two asterisks represent non significantly (p > 0.05) and significantly (p < 0.05) different results, respectively, according to the t-Test.
Figure 10.
De Brouckere diameter (d4,3) from droplet size distribution of emulsions with 5, 7.5, 10 and 15% (w/w) of miso paste and standard emulsion, measured 1 and 30 days after emulsion preparation. Different letters indicate significantly different results (p < 0.05) according to Tukey test. One asterisk and two asterisks represent non significantly (p > 0.05) and significantly (p < 0.05) different results, respectively, according to the t-Test.
Figure 10.
De Brouckere diameter (d4,3) from droplet size distribution of emulsions with 5, 7.5, 10 and 15% (w/w) of miso paste and standard emulsion, measured 1 and 30 days after emulsion preparation. Different letters indicate significantly different results (p < 0.05) according to Tukey test. One asterisk and two asterisks represent non significantly (p > 0.05) and significantly (p < 0.05) different results, respectively, according to the t-Test.
Figure 11.
Sauter diameter (d3,2) from droplet size distribution of emulsions with 5, 7.5, 10 and 15% (w/w) of miso paste and standard emulsion, measured 1 and 30 days after emulsion preparation. Different letters indicate significantly different results (p < 0.05) according to Tukey test. Two asterisks represent non significantly (p > 0.05) and significantly (p < 0.05) different results, respectively, according to the t-Test.
Figure 11.
Sauter diameter (d3,2) from droplet size distribution of emulsions with 5, 7.5, 10 and 15% (w/w) of miso paste and standard emulsion, measured 1 and 30 days after emulsion preparation. Different letters indicate significantly different results (p < 0.05) according to Tukey test. Two asterisks represent non significantly (p > 0.05) and significantly (p < 0.05) different results, respectively, according to the t-Test.
Figure 12.
Comparison of droplet size distribution of emulsions with 7.5% (w/w) of miso paste, one of them with 0.1% (w/w) of psyllium husk, measured 1 (a) and 30 (b) days after emulsion preparation.
Figure 12.
Comparison of droplet size distribution of emulsions with 7.5% (w/w) of miso paste, one of them with 0.1% (w/w) of psyllium husk, measured 1 (a) and 30 (b) days after emulsion preparation.
Figure 13.
Span parameter from droplet size distribution of emulsions with 7.5% (w/w) of miso paste, one of them with 0.1% (w/w) of psyllium husk, measured 1 (a) and 30 (b) days after emulsion preparation. Different letters or two asterisks indicate significantly different results (p < 0.05) according to t-test. One asterisk indicates that samples did not present significantly different results (p > 0.05) according to t-test.
Figure 13.
Span parameter from droplet size distribution of emulsions with 7.5% (w/w) of miso paste, one of them with 0.1% (w/w) of psyllium husk, measured 1 (a) and 30 (b) days after emulsion preparation. Different letters or two asterisks indicate significantly different results (p < 0.05) according to t-test. One asterisk indicates that samples did not present significantly different results (p > 0.05) according to t-test.
Figure 14.
De Brouckere diameter (d4,3) from droplet size distribution of emulsions with 7.5% (w/w) of miso paste, one of them with 0.1% (w/w) of psyllium husk, measured 1 (a) and 30 (b) days after emulsion preparation. Different letters or two asterisks indicate significantly different results (p < 0.05) according to t-test. One asterisk indicates that samples did not present significantly different results (p > 0.05) according to t-test.
Figure 14.
De Brouckere diameter (d4,3) from droplet size distribution of emulsions with 7.5% (w/w) of miso paste, one of them with 0.1% (w/w) of psyllium husk, measured 1 (a) and 30 (b) days after emulsion preparation. Different letters or two asterisks indicate significantly different results (p < 0.05) according to t-test. One asterisk indicates that samples did not present significantly different results (p > 0.05) according to t-test.
Figure 15.
Sauter diameter (d3,2) from droplet size distribution of emulsions with 7.5% (w/w) of miso paste, one of them with 0.1% (w/w) of psyllium husk, measured 1 (a) and 30 (b) days after emulsion preparation. Different letters or two asterisks indicate significantly different results (p < 0.05) according to t-test. Two asterisks indicate that samples presented significantly different results (p > 0.05) according to t-test.
Figure 15.
Sauter diameter (d3,2) from droplet size distribution of emulsions with 7.5% (w/w) of miso paste, one of them with 0.1% (w/w) of psyllium husk, measured 1 (a) and 30 (b) days after emulsion preparation. Different letters or two asterisks indicate significantly different results (p < 0.05) according to t-test. Two asterisks indicate that samples presented significantly different results (p > 0.05) according to t-test.
Figure 16.
Changes in backscattering profiles (ΔBS) as a function of sample height of emulsions with 5, 7.5, 10 and 15% (w/w) of miso paste and standard emulsion, measured 1 day (a) and 30 days (b) after emulsion preparation. ΔBS profiles for 7.5% (w/w) of miso paste, one of them with 0.1% (w/w) of psyllium husk, measured 1 day (c) and 30 days (d) after emulsion preparation are also shown.
Figure 16.
Changes in backscattering profiles (ΔBS) as a function of sample height of emulsions with 5, 7.5, 10 and 15% (w/w) of miso paste and standard emulsion, measured 1 day (a) and 30 days (b) after emulsion preparation. ΔBS profiles for 7.5% (w/w) of miso paste, one of them with 0.1% (w/w) of psyllium husk, measured 1 day (c) and 30 days (d) after emulsion preparation are also shown.
Figure 17.
Total phenolic compounds (TPC) and antioxidant capacity (FRAP and DPPH methods) of standard and 7.5% (w/w) miso emulsions, expressed as mg of gallic acid equivalents per 100 g of emulsion. Different letters indicate significantly different results (p < 0.05) according to t-test.
Figure 17.
Total phenolic compounds (TPC) and antioxidant capacity (FRAP and DPPH methods) of standard and 7.5% (w/w) miso emulsions, expressed as mg of gallic acid equivalents per 100 g of emulsion. Different letters indicate significantly different results (p < 0.05) according to t-test.
Table 1.
Food pathogens and contaminant microorganisms used to test antimicrobial activity of grass pea sweet miso by drop test.
Table 1.
Food pathogens and contaminant microorganisms used to test antimicrobial activity of grass pea sweet miso by drop test.
Group | Microorganism | Library Code | Optimal Growth Conditions |
---|
Nutritive Medium | Temperature |
---|
Food pathogens | Listeria innocua | BISA 3008 | Blood Heart Infusion (BHI) | 38 °C |
Staphylococcus aureus | BISA 3966 |
Escherichia coli | BISA 3967 |
Salmonella Typhimurium | BISA 3969 |
Bacillus cereus | BISA 4043 | 25 °C |
Typical Mendes Gonçalves’ contaminants | Lactiplantibacillus plantarum | BISA 4386 | De Man, Rogosa and Sharpe (MRS) |
Zygosacharomyces parabaillii | ISA 1307 | Yeast extract, peptone, dextrose and fructose (YPDF) |
Pichia membranifaciens | ISA 1454 |
Debaryomyces hansenii | ISA 1509 |
Zygosacharomyces bisporus | ISA 2187 |
Candida stelata | ISA 2339 |
Zygosacharomyces baillii | ISA 2422 |
Table 2.
Microbial analyses and methodologies performed for evaluating the microbial stability of emulsions.
Table 2.
Microbial analyses and methodologies performed for evaluating the microbial stability of emulsions.
Analysis | Methodology |
---|
Total mesophiles quantification | ISO 6610 |
Total molds and yeasts quantification | NP 3277-1 |
Quantification of total lactic acid bacteria | ISO 16649-2 |
Quantification of Escherichia coli | ISO 6888 |
Detection of Listeria monocytogenes | ISO 11290 |
Detection of Salmonella sp. | ISO 6579 |
Table 3.
Chemical composition and bioactivities of grass pea sweet miso and of traditional soybean miso.
Table 3.
Chemical composition and bioactivities of grass pea sweet miso and of traditional soybean miso.
Parameters | Wet Basis |
---|
Grass Pea Sweet Miso | Soybean Miso |
---|
Dry matter (g/100 g) | 43.94 ± 0.36 | 54.78 [47] |
Crude fat (g/100 g) | 0.13 ± 0.04 | 11.00 [46] |
Crude protein (g/100 g) | 6.16 ± 0.17 | 11.98 [47] |
Iron (mg/100 g) | 1.45 ± 0.06 | 4.00 [46] |
Calcium (mg/100 g) | 43.75 ± 6.70 | 150.00 [46] |
Sodium (g/100 g) | 4.17 ± 1.00 | 4.30 [46] |
Sodium chloride (g/100 g) | 10.60 * | 12.12 [47] |
Total phenolic compounds (g of GAE/100 g) | 0.68 ± 0.08 | 1.41–4.26 [48] |
Antioxidant activity (FRAP assay, mg of GAE/100 g) | 16.22 ± 0.13 | 0.60–1.35 [48] |
Antioxidant activity (DPPH assay, mg of GAE/100 g) | 5.29 ± 0.05 | 0.68–1.93 [48] |
Table 4.
Parameters (G′ at 1 Hz and G0N) from mechanical spectra of standard and miso emulsions. Different letters indicate significantly different results (p < 0.05) according to Tukey test. Different amounts of asterisks indicate significantly different results (p < 0.05) according to t-test.
Table 4.
Parameters (G′ at 1 Hz and G0N) from mechanical spectra of standard and miso emulsions. Different letters indicate significantly different results (p < 0.05) according to Tukey test. Different amounts of asterisks indicate significantly different results (p < 0.05) according to t-test.
Emulsions | G′ at 1 Hz (102 Pa) | Plateau Modulus, G0N (102 Pa) |
---|
Vegan mayonnaise | 4.86 ± 0.21 d | 3.98 ± 0.18 d |
5% (w/w) miso | 11.82 ± 0.92 c | 9.48 ± 0.65 c |
7.5% (w/w) miso | 14.93 ± 0.44 ab, * | 12.09 ± 0.35 ab, * |
10% (w/w) miso | 13.57 ± 1.03 bc | 10.88 ± 0.88 bc |
15% (w/w) miso | 16.08 ± 1.06 a | 12.83 ± 0.86 a |
Psyllium | 12.51 ± 0.49 ** | 9.27 ± 0.14 ** |
Table 5.
Parameters (η0, k and m) and adjusted R2 from Williamson’s model adjusted to the flow curves of standard and miso emulsions. Different letters indicate significantly different results (p < 0.05) according to Tukey test. Different amounts of asterisks indicate significantly different results (p < 0.05) according to t-test.
Table 5.
Parameters (η0, k and m) and adjusted R2 from Williamson’s model adjusted to the flow curves of standard and miso emulsions. Different letters indicate significantly different results (p < 0.05) according to Tukey test. Different amounts of asterisks indicate significantly different results (p < 0.05) according to t-test.
Emulsions | η0 (103 Pa.s) | k (103 Pa.s) | m (Dimensionless) | Adjusted R2 |
---|
Vegan mayonnaise | 13.31 ± 0.50 b | 1.39 ± 0.18 | 0.982 ± 0.008 | 0.9971 |
5% (w/w) miso | 31.96 ± 0.96 ab | 1.37 ± 0.07 | 1.016 ± 0.028 | 0.9965 |
7.5% (w/w) miso | 40.85 ± 045 a, * | 1.05 ± 0.07 | 1.000 ± 0.020 | 0.9967 |
10% (w/w) miso | 33.69 ± 8.61 ab | 1.37 ± 0.32 | 0.976 ± 0.012 | 0.9869 |
15% (w/w) miso | 41.89 ± 3.86 ab | 1.82 ± 1.67 | 0.971 ± 0.027 | 0.9947 |
Psyllium | 28.61 ± 8.5 * | 1.25 ± 0.32 | 0.972 ± 0.020 | 0.9917 |
Table 6.
Evolution of microbial counts in 7.5% (w/w) miso emulsion, from day 0 to day 30, after 30 days under refrigeration or under accelerated assay.
Table 6.
Evolution of microbial counts in 7.5% (w/w) miso emulsion, from day 0 to day 30, after 30 days under refrigeration or under accelerated assay.
Microbial Study | Day 0 | Day 30—Refrigeration | Day 30—Accelerated Assay |
---|
Total mesophiles (101 CFU/g) | 285.00 ± 162.64 | 410.00 ± 7.07 | <1 |
Yeasts and molds (102 CFU/g) | 155.00 ± 35.36 | 56.00 ± 7.07 | <1 |
Lactic acid bacteria (CFU/g) | <10 | 57.50 ± 3.54 | <10 |
E. coli (CFU/mL) | <10 | <10 | <10 |
Listeria monocytogenes (in 25 g) | Absent | Absent | Absent |
Salmonella sp. (in 25 g) | Absent | Absent | Absent |