Proteomic Analysis of Exosomes during Cardiogenic Differentiation of Human Pluripotent Stem Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Human Pluripotent Stem Cell Culture
2.2. Cardiac Differentiation
2.3. Exosome Isolation and Characterization
2.4. Size and Morphological Analysis
2.5. RNA Extraction, RT-PCR and Quantitative PCR Analysis
2.6. Western Blot Analysis
2.7. Proteomic Analysis
2.8. Addition of Exosomes to Differentiating Cells
2.9. Flow Cytometry
2.10. Statistical Analysis and Experimental Design
3. Results
3.1. Human Pluripotent Stem Cell-Secreted Exosome Collection and Characterization
3.2. Proteomic Cargo of Exosomes during Cardiogenic hPSC Differentiation
3.3. Exosomes Contain Proteins which Influence hPSC Differentiation
3.4. Effect of Exosomes on Differentiation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ashok, P.; Tzanakakis, E.S. Proteomic Analysis of Exosomes during Cardiogenic Differentiation of Human Pluripotent Stem Cells. Cells 2021, 10, 2622. https://doi.org/10.3390/cells10102622
Ashok P, Tzanakakis ES. Proteomic Analysis of Exosomes during Cardiogenic Differentiation of Human Pluripotent Stem Cells. Cells. 2021; 10(10):2622. https://doi.org/10.3390/cells10102622
Chicago/Turabian StyleAshok, Preeti, and Emmanuel S. Tzanakakis. 2021. "Proteomic Analysis of Exosomes during Cardiogenic Differentiation of Human Pluripotent Stem Cells" Cells 10, no. 10: 2622. https://doi.org/10.3390/cells10102622