Xinghong Yao
Ye Zeng- 1Radiation Oncology Key Laboratory of Sichuan Province, Department of Radiotherapy, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, Affiliated Cancer Hospital of University of Electronic Science and Technology of China, Chengdu, China
- 2Institute of Biomedical Engineering, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China
Tumour progression and metastasis remain the leading causes of cancer-related death worldwide. Tumour angiogenesis is essential for tumour progression. The vasculature surrounding tumours is not only a transport channel for nutrients, oxygen, and metabolites, but also a pathway for metastasis. There is a close interaction between tumour cells and endothelial cells in the tumour microenvironment. Recent studies have shown that tumour-associated endothelial cells have different characteristics from normal vascular endothelial cells, play an important role in tumour progression and metastasis, and are expected to be a key target for cancer therapy. This article reviews the tissue and cellular origin of tumour-associated endothelial cells and analyses the characteristics of tumour-associated endothelial cells. Finally, it summarises the role of tumour-associated endothelial cells in tumour progression and metastasis and the prospects for their use in clinical anti-angiogenic therapy.
1 Introduction
Angiogenesis was introduced by British surgeon John Hunter over 200 years ago to describe the growth of blood vessels in reindeer antlers (Ren et al., 2019). In 1971, Judah Folkman proposed that tumour growth is entirely dependent on angiogenesis (Carmeliet and Jain, 2000).
Angiogenesis is essential for tumour progression. Without blood vessels, tumours remain a mass of cells less than 2 mm in diameter (Ribatti, 2008). The blood vessels surrounding the tumour not only supply nutrients, oxygen and metabolites, but also provide a pathway for tumour cells to metastasise. Therefore, angiogenesis has become a key target for cancer therapy, and several anti-angiogenic drugs/inhibitors have been developed. However, anti-angiogenic therapy is a double-edged sword, with the positive benefits of anti-angiogenic drugs such as reduction of tumour size and prolonged the survival as giving hope to patients and the negative side effects such as hypertension, proteinuria, bleeding, thrombosis and hypothyroidism leaving clinicians conflicted (Li et al., 2022; Motzer et al., 2022; Lee N. et al., 2023). A major dilemma facing clinical anti-angiogenic therapy today is how to maximise treatment efficacy and minimise side effects.
The success or failure of the tumour metastatic cascade response is highly dependent on the interactions between components of the tumour and the local microenvironment through a variety of bioactive molecules such as growth factors, cytokines, chemokines, and microRNAs (Sellner et al., 2022). A particular site of intercellular communication within the tumour is the vasculature. Within the vasculature, tumour cells come into direct contact with tumour-associated endothelial cells (TECs). Tumour-endothelial interactions not only promote malignant vascularisation but also influence the proliferative and metastatic potential of the malignant cells (Hashemi et al., 2022).
VEGF-A (referred to as VEGF) is the major angiogenesis-inducing molecule released by tumour cells (Qi et al., 2023). Excess VEGF in tumours can lead to excessive vascular permeability and increased inter-tissue osmotic pressure, resulting in disorganised vascular architecture. The tumour vasculature is complex, unstratified and disorganized (Balan et al., 2019). In turn, TECs can release vascular secretory factors that promote tumour progression (Butler et al., 2010). TECs can secrete biglycan, which facilitates the metastasis (Maishi et al., 2016).
TECs are found on the inner surface of tumour vessels and are previously considered to be genetically stable (Hillen and Griffioen, 2007). In fact, TECs are distinct from normal vascular endothelial cells and their properties can be exploited in translational research for cancer intervention and treatment. Currently, tumour-derived primary endothelial cells have emerged as a more representative way to assess tumour angiogenesis (Liu et al., 2020). This review focuses on an overview of the origin, features, and role of TECs in tumour progression and metastasis, and their potential application in clinical anti-angiogenic therapy.
2 Origin of TECs
2.1 Tumour blood vessels
Tumor blood vessels are the major source of TECs. TECs can be isolated using the endothelial markers, but they possess some specific characteristics, including the absence of von Willebrand factor (vWF), which distinguishes them from normal vascular endothelial cells (Naschberger et al., 2011).
TECs including tumour-derived vascular endothelial cells (TDVEC) and tumour-derived lymphatic endothelial cells (TDLEC) have been identified in tumours. TEVEC isolated from malignant gliomas express an enriched angiogenesis-related gene profile, whereas TDLEC express interferon-alpha and interferon-beta, which may be involved in inflammation and immune cell recruitment (Carlson et al., 2021). Comparison of a mouse model of endogenous glioma with a patient derived orthotopic xenograft (PDOX) model showed that tumour-associated vascular endothelium is distinct from normal brain endothelium and exhibits extensive heterogeneity (Carlson et al., 2021).
Delivery of extracellular vesicles or non-coding RNAs into the tumour microenvironment plays an essential role in tumour angiogenesis (Li et al., 2021). After overexpression of miR-9, vascular endothelial cells can possess enhanced angiogenic ability, and the generated exosomes followed anti-angiogenic therapy can in turn promote the vascular mimicry of tumour cells and the progression of the abnormal tumour vasculature (Zeng et al., 2019). The extracellular vesicles from the lung cancer cell line A549 cells can induce abnormal angiogenesis by downregulating transient receptor potential vanilloid 4 (TRPV4) and activating Rho/Rho kinase/YAP/VEGFR2 pathways (Guarino et al., 2021). The mechanisms by which the tumour microenvironment modifies endothelial cells are still poorly understood.
2.2 Tumour stem cells
Tumour stem cells may be involved in angiogenesis to form tumour vasculature. Cells derived from tumour to endothelial transdifferentiation express typical endothelial marker CD31 and vWF and stem cell markers such as Nestin and CD133, and cells contributed to vasculogenic mimicry lack CD34 and CD31 expression (He et al., 2012; Maddison et al., 2023).
Glioblastoma endothelial cells have a similar expression profile to other tumour cells. Glioblastoma stem cells positive for the stem cell marker CD133 may have an endothelial-like phenotype and function (Ricci-Vitiani et al., 2010; Wang et al., 2010). However, it has been reported that TDVEC are rarely found in glioblastoma vasculature (Rodriguez et al., 2012). Nonetheless, glioma stem cells can differentiate into perivascular cells and thus support tumour angiogenesis and progression (Cheng et al., 2013). The use of a combinatorial treatment of RoboN and the monoclonal anti-SDF-1 antibody effectively attenuated the activity of hemangioblast derived-endothelial cell precursors and tumor neovascularization (Shenoy et al., 2023). CD44/CD44v6 might play a vital role in formation of cancer stem cells and angiogenesis (Wang et al., 2018).
Although transdifferentiation of tumor stem cells to enodothelial cells have been suggested in vasculogenic mimicry, whether tumour stem cells are origin of TECs and the transformation mechanism remains unclear.
2.3 Endothelial progenitor cells
Endothelial progenitor cells recruited from bone marrow have the capacity to differentiate into mature vascular endothelial cells (Zhou et al., 2013). Endothelial CD34+CD45– progenitors formed entire blood vessels in wounds, inflamed skin, and tumors (Patel et al., 2017). The CD34 were progressively lose in endothelial progenitor cells during cultivation and maturation (Delia et al., 1993).
CD133+ bone marrow cells, a subset of CD34+ progenitors, can retain high levels of VEGFR2 expression while positively expressing VE-cadherin, vWF, and platelet endothelial cell adhesion molecule 1 (PECAM1, CD31) (Quirici et al., 2001). Another study suggested that CD133+ cells were unable to differentiate into mature endothelial cells, whereas CD34+ endothelial progenitors could (Lu et al., 2007). It is possible that CD133+/CD34+ endothelial progenitor cells are a stable origin of TECs. However, the specific mechanism by which the endothelial progenitor cells differentiate into TECs is not yet known.
3 Characteristics of TECs
3.1 Genetic instability
TECs from xenografts, such as human melanoma and liposarcoma, have larger nuclei than normal vascular endothelial cells from skin and adipose, and cytogenetic abnormalities (Hida et al., 2004). Lymphatic-specific chromosomal translocations have also been found in microvascular endothelial cells of B-cell lymphomas (Streubel et al., 2004). In addition to tumour-resident TECs, aneuploid CD31+ circulating TECs are found in the peripheral blood of breast cancer patients (Lin et al., 2017). TECs isolated from xenografted human epithelial tumours are CD133+ cells, have a higher frequency of aneuploidy than CD133-cells and are genetically unstable (Akino et al., 2009).
Hypoxia-induced reactive oxygen species and VEGF signalling promote chromosomal abnormalities of TECs (Kondoh et al., 2013). Vascular endothelial cells may receive tumour genetic material (DNA/chromosomes) delivered by tumour cell-derived apoptotic vesicles (Bergsmedh et al., 2001; Ehnfors et al., 2009), resulting in altered cytogenetics.
Cell fusion between transformed cells and normal stem cells may be important in the genomic transfer and generation of reprogrammed somatic cell hybrids with self-renewing highly malignant phenotype (Glinsky, 2005). The formation of cell-in-cell structures (a type of unique structure with one or more cells within another one) may promote genetic transfer and contribute to genomic instability and malignancy of tumor cells (Wang et al., 2023). Thus, fusion of bone marrow-derived endothelial progenitor cells with TECs might be responsible for genetic instability.
3.2 Genetic heterogeneity
TECs are composed of heterogeneous cell populations (Hennigs et al., 2021). Single-cell RNA sequencing has also demonstrated the heterogeneity of TECs in mouse and human samples (Goveia et al., 2020). The heterogeneity of TECs is influenced by tumour type, extracellular environment and epigenetic regulation (Aird, 2009). TECs have distinct and heterogeneous gene expression patterns compared to normal vascular endothelial cells (Hennigs et al., 2021). Genes involved in the regulation of angiogenesis, cell proliferation and motility, stemness and drug resistance are differentially upregulated in TECs (Ohga et al., 2012). TECs express high levels of VEGFR1 and VEGFR2, which enhances cellular responsiveness to VEGF and angiogenesis (Matsuda et al., 2010). They have the capacity for survival in serum-free medium (Bussolati et al., 2003). Compared to normal colorectal mucosal endothelium, 46 genes were specifically elevated in endothelial cells isolated from colorectal cancer, including MMP2 and MMP11, and collagen types I, III and VI (St Croix et al., 2000). A map of single-cell sequencing in hepatocellular carcinoma (HCC) shows that in TECs, extracellular matrix organization genes such as COL4A2 and SPARC were upregulated, liver sinusoidal endothelial cell (LSEC) markers such as CLEC4G did not express, while macro-vascular endothelial cell (MVEC) markers such as PECAM1 (CD31), AQP1 and CD34 were also upregulated (Aizarani et al., 2019). Furthermore, HCC LSECs express plasmalemma vesicle-associated protein (PLVAP), making them less permeable and potentially limiting the entry of lymphocytes and tumour-derived antigens (Rantakari et al., 2015). PLVAP is considered as a major factor influencing the permeability of endothelial cells, also upregulated within pro-angiogenic or pro-inflammatory responses (Denzer et al., 2023).
3.3 Anoikis resistance
Anoikis (Anchorage-Dependent Cell Death) refers to the death of normal adnexal cells that are left suspended for long periods and become ‘homeless’. Non-coding RNAs are closely related to anoikis resistance of cancer cells (Shi et al., 2022). TECs with high expression of miR-145 have an increased ability to resist anoikis (Hida et al., 2017). Circulating TECs with high expression of Bcl-2 also have increased resistance to anoikis (Yadav et al., 2015). The increased resistance of TECs to anoikis enhances their ability to survive after entry into the circulation, while improving cell adhesion and promoting tumour metastasis (Yadav et al., 2015). TECs interacting with tumour cells can protect the latter from anoikis in circulation and help them to migrate to distant organs (Pyaskovskaya et al., 2021). Silencing of syndecan-4 led to an inhibition of the proliferative, invasive and angiogenic capacity of anoikis-resistant endothelial cells (Onyeisi et al., 2020).
3.4 Abnormal metabolism
Angiogenesis of lung TEC is closely associated with glycolysis and oxidative stress (Wu et al., 2018). Inhibition of glycolysis tightened the vascular barrier and inhibited the expression of cancer cell adhesion molecules in endothelial cells (Cantelmo et al., 2016). Pharmacological blockade of cyclooxygenase-2 (COX-2) by parixibox restored the glucose metabolism level (particularly glycolysis) in TECs, reducing the VEGF (Zhang et al., 2018). Inhibition of the glycolytic activator PFKFB3 tightened the vascular barrier by reducing VE-cadherin endocytosis in TECs, contributing to the reduction of metastasis by normalizing tumor vessels (Cantelmo et al., 2016). Deficiency of endothelial TRPM7 inhibited postnatal retinal angiogenesis in mice might through normalized glycolytic metabolism (Wu et al., 2023). Lactate activated the major stroma components in gastric cancer mesenchymal stem cells and promote their migration (Tao et al., 2023). Unlike normal endothelial cells, TECs proliferate in lactic acidic via upregulation of carbonic anhydrase 2 (CA2) (Annan et al., 2019). The mechanism by which TECs survive in a high-lactate environment and participate in angiogenesis is required to be further explored.
3.5 Resistance to anticancer drugs
Following first-line chemotherapy, the proportion of ABCB1-postive TEC in urothelial carcinoma was increased, which conferred drug resistance to the tumor (Kikuchi et al., 2020).
TECs isolated from highly metastatic human melanoma cell xenografts express high levels of multidrug resistance protein 1 (MDR1) and are resistant to the anticancer drug paclitaxel (Akiyama et al., 2012). CD105+ TECs isolated from hepatocellular carcinoma were resistant to doxorubicin and 5-fluorouracil (Xiong et al., 2009). The PD-L1+ aneuploid circulating TECs exhibit resistance to the checkpoint blockade immunotherapy in advanced non-small cell lung cancer patients (Zhang et al., 2020).
TECs highly express stemness markers such as CD105 and CD146 (St Croix et al., 2000; Otsubo et al., 2014). The stem cell properties of TECs may be responsible for the development of drug resistance, but further clarification is required.
3.6 Adaptive to abnormal mechanical microenvironment
Tumour-associated vasculature is characterised by irregular morphology and structure, dysfunction, and reduced flow and leakage in comparison to normal vessels. The stiffness of tumour-derived extracellular matrix is increased, leading to high interstitial pressure (Paszek et al., 2005). Increased interstitial pressure and reduced blood flow within the tumour vasculature reduces shear stress and inhibits leukocyte rolling but promote their adhesion to the endothelium (Finger et al., 1996). Endothelial cells can sense the stiffness of the extracellular matrix. In contrast to a soft stroma, a hard stroma led to dysfunction of the endothelial monolayer, independently from inflammatory signalling (Onken et al., 2014). The composition of extracellular matrix such as geltrex and collagen I is critical for endothelial cell function (Riddle et al., 2022). It remains unknown whether the mechanical responsiveness of TECs differs from that of normal vascular endothelial cells.
The major characteristics of TEC are summarized in Table 1.
TABLE 1. Characteristics of TECs.
4 TECs in tumour progression and metastasis
Tumour blood vessels, with abnormal thin-wall, contribute to metastasis (Oka et al., 2023). The immature nature of tumour vessels, with the absence of smooth muscle cells or pericytes, allows tumour cells to readily invade tumour vessels (Morikawa et al., 2002).
TECs may also provide signals that actively promote metastasis. CXCR7 regulated CXCL12-mediated migratory cues, driving CXCR4+CXCR7+ metastasis and tissue invasion (Zabel et al., 2009). However, in hepatocellular carcinoma patients, CXCR4 affected overall survival, but not CXCR7 (Neve Polimeno et al., 2015). Conditionally deleted the CXCR7 from vascular endothelium of adult mice elevated number of circulating tumor cells and more spontaneous metastases (Stacer et al., 2016). Transendothelial migration of tumour cells was increased after VEGF disrupted the glycocalyx on the surface of vascular endothelial cells (Xia et al., 2022; Zeng et al., 2022). Heparan-sulfate proteoglycans (HPSE) digestion-generated heparan sulfate fragments of about 10 kDa in B16B15b melanoma cell stimulated melanoma migration and angiogenesis, which were possibly due to signaling by some other unknown heparin-binding growth factor(s) (Roy and Marchetti, 2009).
TECs can transform tumour cells into more tumourigenic, invasive and chemoresistant cells (Cao et al., 2017; Hoarau-Véchot et al., 2022). In a bio-3D printed in vitro vascular model of neuroblastoma, the invasive behaviour of neuroblastoma cells co-cultured with human umbilical vein endothelial cells was significantly increased (Ning et al., 2022). By inhibiting the expression of lectin-like oxidised LDL receptor 1 (LOX-1) in TECs, the LOX-1 enahnced neutrophil migration was inhibited, and thus the formation of high metastatic-tumor microenvironment was inhibited and lung metastasis of tumour cells can be suppressed (Tsumita et al., 2022).
In addition, TECs can accelerate lung metastasis of tumour cells with low metastatic potential by releasing the vascular secretory factor biglycan (Maishi et al., 2016). TECs express high levels of adhesion molecules and biglycan, which allow vascular endothelial cells to bind to tumour cells, protecting them from circulating anoikis and promoting their metastasis to distant organs (Yadav et al., 2015).
5 TECs in anti-angiogenic therapy
Anti-angiogenic drugs are currently used in a combination therapeutic model with immunotherapy and radiotherapy, which demonstrated promising anti-tumor activity in various advanced solid tumors (Shen et al., 2023). Anti-angiogenic therapies alter the immunologically permissive microenvironment of the tumour by reducing the number of regulatory T cells and myeloid-derived suppressor cells (Wallin et al., 2016; Hegde et al., 2018). However, anti-VEGFA therapy is a direct reduction in the rate of tumour growth rather than an induction of tumour regression, and some drugs slow tumour growth without necessarily causing tumour shrinkage (Bagri et al., 2010). In patients with advanced EGFR-mutated non-small cell lung cancer, longer but not significant progression-free survival (PFS) was observed in the erlotinib plus bevacizumab compared with the erlotinib alone (Lee Y. et al., 2023).
Despite the benefits, anti-angiogenic therapies face challenges. Complications and adverse events such as hypertension, hand-foot syndrome, proteinuria and thyroid dysfunction can occur due to the adverse effect of anti-angiogenic drugs on normal blood vessels, as well as the possibility of drug resistance, which often requires drug replacement (Li et al., 2022; Yuan et al., 2022; Lee N. et al., 2023).
Long-term anti-angiogenic therapy induces tumour hypoxia and invasiveness (Pàez-Ribes et al., 2009). Hypoxia also induces tumour angiogenesis through the recruitment of bone marrow-derived endothelial progenitor cells (Gao et al., 2009). Recurrence and metastasis after initial tumour response have also been reported with anti-angiogenic therapy (Mancuso et al., 2006; Allegra et al., 2009; Ebos et al., 2009; Toda et al., 2021). Exosomes generated by TECs in response to anti-angiogenic drugs may also promote tumour cell vascular mimicry and the development of aberrant tumour vasculature (Zeng et al., 2019; Zeng and Fu, 2020). This provides in vitro evidence and possible mechanisms by which anti-angiogenic therapy may promote metastasis.
6 Conclusion and prospects
TECs are mainly derived from tumour vasculature (Figure 1). They may be formed by uptake of extracellular vesicles or non-coding RNAs from tumour cells and may undergo transdifferentiation from tumour stem cells and endothelial progenitor cells, although the exact mechanism of transformation requires further investigation. The formation of TECs may involve fusion between transformed cells and normal stem cells.
FIGURE 1. Potential role of tumour-associated endothelial cells (TECs) in tumour angiogenesis and metastasis. TECs are mainly derived from tumour vasculature. They may be formed by uptake of extracellular vesicles (EVs) or non-coding RNAs from tumour cells (TCs) and may undergo transdifferentiation from tumour stem cells and endothelial progenitor cells. (A–D) Following the occurrence of tumor, the normal vascular endothelial cells (ECs) are transformed into TECs, which facilitate the infiltration of tumor cells into microcirculation and metastasis through bloodstream. The circulation tumor cells may induce the transformation of endothelial cells in distal vessels into TECs, and aid to their adhesion and invasion for metastatic foci formation.
TECs are distinctly different from normal endothelial cells, characterized by genetic instability, genetic heterogeneity, anoikis resistance, abnormal metabolism, resistance to anticancer drugs and adaptation to an abnormal mechanical microenvironment (Figure 2). TECs play an important role in tumour progression and metastasis. Increased evidences suggest that TECs are more promising targets than normal endothelial cells. Circulating TECs may be mesenchymal TECs shed from the neoplastic vasculature (McGuire et al., 2012; Lin, 2020). Circulating TECs reduction after anti-angiogenic therapy and surgical tumour resection (Mehran et al., 2014; Zhang et al., 2021) suggests that circulating TECs should be more specific for cancer and more suitable for prognosis, but it is unclear which precise markers is specific for TECs and appropriate for clinical application.
FIGURE 2. Major characteristics of TECs.
Furthermore, the complexity of the tumour microenvironment and its intricate details remain unclear, and there is a lack of uniform standards for the detection of TECs. A comprehensive description of the tumour microenvironment and a better understanding of the mechanism underlying how TECs form and function will facilitate the design of novel approaches for overcoming resistance and monitoring the efficacy of anti-angiogenic therapy.
Author contributions
YZ contributed to the conception and design of the work. YZ, and XY drafted the manuscript. All authors contributed to the article and approved the submitted version.
Funding
Supported by the National Natural Science Foundation of China (12272246), the Key Research and Development Projects of Sichuan Province (2023YFS0075), and the Medical Science and Technology Project of Health Commission of Sichuan Province (21PJ116).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
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Keywords: tumour, tumour-associated endothelial cells, characteristics, metastasis, anti-angiogenic therapy
Citation: Yao X and Zeng Y (2023) Tumour associated endothelial cells: origin, characteristics and role in metastasis and anti-angiogenic resistance. Front. Physiol. 14:1199225. doi: 10.3389/fphys.2023.1199225
Received: 03 April 2023; Accepted: 23 May 2023;
Published: 14 June 2023.
Edited by:
Agustín Guerrero-Hernández, National Polytechnic Institute of Mexico (CINVESTAV), MexicoCopyright © 2023 Yao and Zeng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Ye Zeng, ye@scu.edu.cn