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Electrophoretic patterns of proteins from Paragonimus westermani in early developmental stages
Boong Huer,Suk Il Kim,Shin Yong Kang and Seung-Yull Cho
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 151, Korea.
Abstract
In order to observe the protein compositions of soluble extracts of P. westermani, and their changes during early developmental stages, the crude saline extracts of 4, 6, 8, 10 and 12 week-old worms which were harvested from experimentally infected dogs were analysed by disc-PAGE. The results were as follows: A total of 15 bands were identified from electrophoregrams of respective developmental stages. Of them, 5 bands were recognized throughout the developmental stages. The number and protein amount of identified bands changed according to the worm development from 4 weeks to 12 weeks. However, the banding patterns of 4 and 6 week-old worms and 8 and 10 week-old worms were similar each other. Of 15 identified bands, band 1 was recognized only in 12 week-old worms whereas bands 3, 4, 8, 9, 10, 11 and 15 gradually lowered their amount according to development to disappear in 12 week-old. In addition, band 5 became a major band in 12 week-old while band 6 turned to a minor band at tha same age. The possible relations of changing patterns of protein bands with worm development were discussed.
Figures
Fig. 1 Electrophoretic patterns and their densitometric profile of saline extracts from P. westermani. All identified bands are numbered, and those marked with open arrow heads are the major bands. (1) Whole worm extract of 4 week-old Paragonimus westermani (=Pw4WWE) (2) Pw6WWE (3) Pw8WWE (4) Pw10WWE (5) Pw12WWE (6) Somatic extract of 12 week-old P. westermani (Pw12SME)
Fig. 2 Comparison of electrophoretic patterns of saline extracts from early developmental stages of P. westermani. All identified bands were numbered; and changing bands were marked with 3 kinds of arrow heads. Open arrow head: Progressively decreasing stainability with development
Closed arrow head: Newly appeared in 12 week-old
Dashed arrow head: Those turned from major band to minor, or vise versa
Table 1 Mean Rf values of protein bands identified in disc-PAGE of 5 developmental stages of P. westermani
Table 2 Relative composition of protein bands in disc-PAGE as measured by densitometric tracing
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