Steroid-Induced Ocular Hypertension Model in the Mice.

Kim, You Ra; Kang, Wan Seok; Kweon, Eui Yong; Cho, Nam Chun; Lee, Dong Wook

doi:2014.55.8.1202

J Korean Ophthalmol Soc > Volume 55(8); 2014 > Article

Original Article

Journal of the Korean Ophthalmological Society 2014;55(8):1202-1207.
DOI: https://doi.org/10.3341/jkos.2014.55.8.1202 Published online August 15, 2014.

Steroid-Induced Ocular Hypertension Model in the Mice.

You Ra Kim, Wan Seok Kang, Eui Yong Kweon, Nam Chun Cho, Dong Wook Lee

Department of Ophthalmology, Chonbuk National University Hospital, Chonbuk National University Medical School, Jeonju, Korea. ldw@jbnu.ac.kr

생쥐를 이용한 스테로이드 유발 고안압 모델

김유라⋅강완석⋅권의용⋅조남천⋅이동욱

Department of Ophthalmology, Chonbuk National University Hospital, Chonbuk National University Medical School, Jeonju, Korea

Abstract

PURPOSE
To determine whether rat eyes develop increases in intraocular pressure (IOP) in response to a topically applied corticosteroid and to investigate the relationship between ocular hypertension and apoptosis of retinal ganglion cells. METHODS: IOP was monitored by rebound tonometry in a group of 10 rats that received topically administered dexamethasone in both eyes (experimental) and in another group of 5 rats that received artificial tears (control) three times daily for 4 weeks after the establishment of baseline IOP values. Only eyes that increased by more than 50% compared with the basal IOP were administered once per day for 5 weeks. After 8 weeks, selective immunofluorescence stain for retinal ganglion cells and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stain were conducted. RESULTS: Among 20 experimental eyes, 11 eyes (55%) showed a greater than 50% increase in IOP compared with basal IOP. After 8 weeks, the mean IOPs for the experimental and control groups were 11.8 +/- 1.4 mm Hg and 18.5 +/- 1.0 mm Hg, respectively (p < 0.01). The counts of central retinal ganglion cells (RGCs) were 2718 +/- 240 and 2612 +/- 443, respectively (p = 0.294). The results of the TUNEL stain also showed no differences. CONCLUSIONS: Rat eyes exhibit a steroid-induced ocular hypertensive response with no local complications. However, maintaining ocular hypertension increased by 50% for two months was not enough to detect changes in RGCs.

Key Words: Ocular hypertension;Retinal ganglion cell;Steroid-induced glaucoma