Open Access, Peer-reviewed
pISSN 1015-4817
eISSN 2289-0963
    Korean Lepr Bull. 2020 Dec;53(1):23-34. Korean.
    Published online Dec 29, 2020.
    https://doi.org/10.33161/klb.2020.53.1.23
    Copyright © 2020 Society of Korean Leprologists
    Original Article

    The Study of Association between Leprosy's Type and Apolipoprotein E Gene in Korean Persons Affected by Leprosy

    Jin-Ho Park, Yun-Ji Kim and Jong-Pill Kim
      • Institute for Leprosy Research, Korean Hansen Welfare Association, Korea.
    • Corresponding author: Jin-Ho Park. (Email: pjh718g@gmail.com )

    Abstract

    Background

    Apolipoprotein E protein is a family of apolipoproteins that play an important role in lipid metabolism. Apolipoprotein E genotype and single-nucleotide polymorphisms of genes are known to induce neurodegenerative diseases and cardiovascular diseases. Recently, it has been reported that apolipoprotein E is related to Hansen's disease by regulating genes related to lipid metabolism.

    Objective

    In this study, we investigated that relationship of apolipoprotein E genotype with leprosy type and single-nucleotide polymorphism in korean persons affected by leprosy.

    Methods

    The Apolipoprotein E genotype was confirmed using the polymerase chain reaction from korean persons affected by leprosy. Real-time polymerase chain reaction using probes confirmed the rs405509 and rs439401 of Apolipoprotein E. The identified genotype was cross-analyzed with each single-nucleotide polymorphism results and leprosy type by chi-squared test.

    Results

    The distribution of apolipoprotein E genotypes was E3/E3 76.7%, E3/E4 17.4%, E2/E2 2.3%, E2/E3 2.3%, E2/E4 1.2% were in order. The frequencies of the apolipoprotein E allele were identified in the order of ε2 4.1%, ε3 86.6%, and ε4 9.3%. By type of leprosy, ε2 allele increased 2.2 times in multibacillary than pauacibacillary(4.8% vs 2.2%), and ε4 allele decreased 2.8 times in multibacillary than pauacibacillary(6.3% vs 17.4%). ApoE genotype was cross-analyzed with rs405509·rs439401, and there was a statistically significant association.

    Conclusion

    Apolipoprotein E genotype is associated with leprosy types in korean persons affected by leprosy. The research for apolipoprotein E gene is thought to be helpful in basic research on the pathogenesis of leprosy.who underwent surgery.

    Keywords
    Apolipoproteins E; Leprosy; Mycobacterium leprae; Single-nucleotide polymorphism

    Tables

    Table 1
    Distribution of ApoE genotypes

    Table 2
    Relationship between ApoE genotypes and leprosy types

    Table 3
    Comparison of ApoE gene allele by leprosy types

    Table 4
    Relationship between each ApoE allele group and leprosy types

    Table 5
    Relationship between ApoE single-nucleotide polymorphisms (SNPs) and leprosy types

    Table 6
    Relationship between ApoE single-nucleotide polymorphisms (SNPs) and genotypes

    Table 7
    Relationship between each ApoE allele group and leprosy types

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    MeSH Terms
    Alleles
    Apolipoproteins E
    Apolipoproteins*
    Cardiovascular Diseases
    Genotype
    Humans
    Leprosy*
    Lipid Metabolism
    Mycobacterium leprae
    Neurodegenerative Diseases
    Polymerase Chain Reaction
    Proteins
    Real-Time Polymerase Chain Reaction
    Thinking
    Metrics
    Share
    Tables
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