Open Access
ARTICLE
Overexpression of inhibin α (1-32) fusion protein promotes apoptosis and cell cycle arrest in a cervical cancer cell model (Hela cells)
Yanhong ZHEN1, Li HAN2, Kailai CAI1, Lijun HUO1, Hasan RIAZ1, Canjie WU1, Aixin LIANG1 , Lei SANG1, Liguo YANG1 *
1
Key laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education. Huazhong Agricultural University, Wuhan,
430070, Hubei, China.
2
College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China
* Address correspondence to: Liguo Yang,
BIOCELL 2014, 38(1), 17-24. https://doi.org/10.32604/biocell.2014.38.017
Abstract
Inhibins play important roles in the reproductive system. To evaluate whether inhibin α (1-32)
fusion protein plays a role in cervical cancer growth, the plasmid pVAX-inhα was constructed and its effect on
proliferation and apoptosis of the human cervical cancer cell line (Hela) was checked by flow cytometry and
real-time PCR. The expression and localization of inhibin α protein were detected by RT-PCR and confocal microscopy which showed that inhibin α protein was expressed and localized in the nucleus of Hela cells. Over expression of inhibin α gene significantly induced cell apoptosis and ceased S phase of cell cycle. Furthermore, cell
proliferation was significantly suppressed 96 h post-transfection and mRNA level of anti-apoptosis genes (Bcl-2,
NFκB) were decreased but pro-apoptosis genes (Bax, wild type p53) and inhibin co receptor (TGFβR3) were
increased, indicating that inhibin, through its co-receptor, might activate apoptotic and cell growth cascades
which regulate proliferation and apoptosis in Hela cells. These results suggest that inhibin α (1-32) fusion protein, located in the cell nucleus, can regulate Hela cells growth and apoptosis by induction of apoptotic pathways
such as NFκB, Bcl-2 and p53 families. These findings may have a significant impact on future research regarding
cervical cancer cell lines
Keywords
Cite This Article
APA Style
ZHEN, Y., HAN, L., CAI, K., HUO, L., RIAZ, H. et al. (2014). Overexpression of inhibin α (1-32) fusion protein promotes apoptosis and cell cycle arrest in a cervical cancer cell model (hela cells). BIOCELL, 38(1), 17-24. https://doi.org/10.32604/biocell.2014.38.017
Vancouver Style
ZHEN Y, HAN L, CAI K, HUO L, RIAZ H, WU C, et al. Overexpression of inhibin α (1-32) fusion protein promotes apoptosis and cell cycle arrest in a cervical cancer cell model (hela cells). BIOCELL . 2014;38(1):17-24 https://doi.org/10.32604/biocell.2014.38.017
IEEE Style
Y. ZHEN et al., "Overexpression of inhibin α (1-32) fusion protein promotes apoptosis and cell cycle arrest in a cervical cancer cell model (Hela cells)," BIOCELL , vol. 38, no. 1, pp. 17-24. 2014. https://doi.org/10.32604/biocell.2014.38.017