The collection of Glenanthe salina Mathis in a freshwater restored wetland in Hamilton County, Ohio is a new county record and represents a significant range extension within the state. For the first time, G. salina was collected from an Ohio saline habitat (Wayne County) and is a new county and an eastern North American inland salt habitat record. Glenanthe salina records from freshwater, saline, and alkaline habitats suggest broad osmoregulation capacity. Collection records suggest that G. salina and Psilopa girschneri colonized saline habitats at Rittman, Ohio during 1996 and 1976, respectively. During the early half of the twentieth century, hyper saline habitat at Rittman, Ohio was colonized by Ephydra gracilis Packard, and E. niveiceps Cresson, which have been commonly reported from saline habitats in western North America.