Molecular Epidemiology of Koi Herpesvirus

Jun Kurita; Kei Yuasa; Takafumi Ito; Motohiko Sano; Ronald P. Hedrick; Marc Y. Engelsma; Olga L. M. Haenen; Agus Sunarto; Edy Barkat Kholidin; Hsin-Yiu Chou; Ming-Chen Tung; Leobert de la Peña; Gilda Lio-Po; Chien Tu; Keith Way; Takaji Iida

doi:10.3147/jsfp.44.59

Research Articles

Molecular Epidemiology of Koi Herpesvirus

Jun Kurita, Kei Yuasa, Takafumi Ito, Motohiko Sano, Ronald P. Hedrick, Marc Y. Engelsma, Olga L. M. Haenen, Agus Sunarto, Edy Barkat Kholidin, Hsin-Yiu Chou, Ming-Chen Tung, Leobert de la Peña, Gilda Lio-Po, Chien Tu, Keith Way, Takaji Iida

Author information

Jun Kurita
Tamaki Station, National Research Institute of Aquaculture, Fisheries Research Agency
Kei Yuasa
Nansei Station, National Research Institute of Aquaculture, Fisheries Research Agency
Takafumi Ito
Tamaki Station, National Research Institute of Aquaculture, Fisheries Research Agency
Motohiko Sano
Nansei Station, National Research Institute of Aquaculture, Fisheries Research Agency
Ronald P. Hedrick
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California
Marc Y. Engelsma
CIDC-Lelystad, Wageningen UR, Fish and Shellfish Diseases Laboratory
Olga L. M. Haenen
CIDC-Lelystad, Wageningen UR, Fish and Shellfish Diseases Laboratory
Agus Sunarto
Fish Health Research Laboratory, Agency for Marine and Fisheries Research
Edy Barkat Kholidin
Jambi Freshwater Aquaculture Development Center
Hsin-Yiu Chou
Department of Aquaculture, National Taiwan Ocean University
Ming-Chen Tung
Department of Veterinary Medicine, National Pingtung University of Science & Technology
Leobert de la Peña
Fish Health Section, Southeast Asian Fisheries Development Center Aquaculture Department (SEAFDEC/AQD)
Gilda Lio-Po
Fish Health Section, Southeast Asian Fisheries Development Center Aquaculture Department (SEAFDEC/AQD)
Chien Tu
Fish Health Laboratory, Animal Health Research Institute
Keith Way
Centre for Environment Fisheries and Aquaculture Science (Cefas)
Takaji Iida
Nansei Station, National Research Institute of Aquaculture, Fisheries Research Agency

Keywords: koi herpesvirus, KHV, Cyprinid herpesvirus-3, CyHV-3, molecular epidemiology, fish herpesvirus, Cyprinus carpio

JOURNAL FREE ACCESS

2009 Volume 44 Issue 2 Pages 59-66

DOI https://doi.org/10.3147/jsfp.44.59

Details

Abstract

Three regions of koi herpesvirus (KHV) genomic DNA were compared for 34 samples from Japan, six from Indonesia, two from Taiwan, one from the Philippines, 13 from the Netherlands, one from the UK, one from the USA and one from Israel. The analyzed genomic regions included known PCR-detection targets (SphI-5, 9/5 and the thymidine kinase gene). The KHVs from Asian countries were very homogeneous, although two variants were noted based on a single nucleotide polymorphism. In contrast, seven variants were found in KHVs from outside of Asia, and although closely related to one another, they were clearly distinct from those from Asian. The results suggest that a clear genetic distinction exists between Asian and European (including each single isolate from the USA and Israel) types of KHV, and that unique types of KHV were independently introduced or emerged in the respective geographic locations.

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