Characterization of Tween® Surfactants by MALDI TOF-MS and High Performance Liquid Chromatography in a Ternary Mobile Phase

Shazia Abrar; Bernd Trathnigg; Sadia Javed; Shumaila Kiran; Tahsin Gulzar

doi:10.3139/113.110590

Open Access Published by De Gruyter November 16, 2018

Characterization of Tween^® Surfactants by MALDI TOF-MS and High Performance Liquid Chromatography in a Ternary Mobile Phase

Charakterisierung von Tween^®-Tensiden mittels MALDI-TOF-MS und Hochleistungsflüssigkeitschromatographie in einer ternären mobilen Phase

Shazia Abrar , Bernd Trathnigg , Sadia Javed , Shumaila Kiran and Tahsin Gulzar

From the journal Tenside Surfactants Detergents

https://doi.org/10.3139/113.110590

Abstract

Different hydrophobic and hydrophilic functionalities are produced in the reaction mixture of Tween^® surfactants which affect the physiochemical properties of the final product. Only hydrophilic fractions and monoesters were separated in the binary mobile phase. MALDI TOF-MS of Tween^® 40 revealed the identification of peaks separated in binary mobile phase (methanol-water). Liquid adsorption chromatography was performed on a gradient system on a Synergi^TM fusion reverse phase column. Mobile phase composition used for this separation was a ternary phase gradient starting from 41 % methanol, 47 % acetone and going to 96 % acetone for the analysis time of 60 minutes. In the ternary mobile phase, gradient all the species such as polyethylene glycol, sorbitan and isosorbide ethoxylates, mono-, di- and triesters of sorbitan and isosorbide ethoxylates were well resolved in an of 60 minutes analysis time. The above separation mechanism can be employed for the analysis of polymeric multifunctional surfactant compositions.

Kurzfassung

Im Reaktionsgemisch von Tween^®-Tensiden werden unterschiedliche hydrophobe und hydrophile Funktionalitäten erzeugt, die die physikalisch-chemischen Eigenschaften des Endproduktes beeinflussen. Nur die hydrophilen Fraktionen und Monoester wurden in der binären mobilen Phase getrennt. Die MALDI-TOF-MS-Analyse von Tween^® 40 lieferte die Identifizierung von Peaks, die in der binären mobilen Phase (Methanol-Wasser) getrennt waren. Die Flüssigadsorptionschromatographie wurde auf einem Gradientensystem auf einer Synergi^TM Fusion-Reversed-Phase-Säule durchgeführt. Die Zusammensetzung der mobilen Phase, die für diese Trennung verwendet wurde, war ein ternärer Phasengradient mit 41 % Methanol, 47 % Aceton und 96 % Aceton bei einer Analysezeit von 60 Minuten. In der mobilen ternären Gradientenphase waren alle Spezies wie Polyethylenglycol, Sorbitan und Isosorbidethoxylate, Mono-, Di- und Triester von Sorbitan und Isosorbidethoxylate in einer Analysezeit von 60 Minuten gut aufgelöst. Der obige Trennmechanismus kann für die Analyse von polymeren multifunktionellen Tensidzusammensetzungen verwendet werden.

Key words: Tween® 40; liquid chromatography; ternary mobile phase; MALDI-TOF MS

Stichwörter: Tween® 40; Flüssigchromatographie; ternäre mobile Phase; MALDI-TOF-MS

*Correspondence address, Dr. Shazia Abrar, Department of Applied Chemistry, Government College University Faisalabad, Pakistan, Tel.: 00923336926912, E-Mail: shaziaabrar@gcuf.edu.pk

Dr. Shazia Abrar completed her Ph.D. studies from University of Graz, Austria. Currently she is working as an Assistant Professor in the Department of Applied Chemistry, Government College University Faisalabad. Her area of research is analytical chemistry, surfactants and detergent, formulation of cosmetic products, physiochemical properties of cosmetic formulation, chemistry.

Dr. Bernd Trathnigg worked as a professor in the Institute of Chemistry, University of Graz, Austria. He worked in the Polymer Chemistry, Organic Chemistry and Materials Chemistry.

Dr. Sadia Javed completed her Ph.D. studies from University of Agriculture Faisalabad Pakistan. Currently she is working as an Assistant Professor in the Department of Biochemistry, Government College University Faisalabad. Her area of research is enzyme biotechnology and biochemistry.

Dr. Shumaila Kiran completed her Ph.D. studies from University of Agriculture Faisalabad, Pakistan. Currently she is working as an Assistant Professor in the Department of Applied Chemistry, Government College University Faisalabad. Her area of research is Green chemistry and natural product chemistry.

Dr. Tahsin Gulzar completed her Ph.D. studies from HEJ Karachi, Pakistan. Currently she is working as an Assistant Professor in the Department of Applied Chemistry, Government College University Faisalabad. Her area of research is organic chemistry and natural product chemistry.

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Received: 2018-01-22

Accepted: 2018-06-11

Published Online: 2018-11-16

Published in Print: 2018-11-16

This work is licensed under the Creative Commons Attribution 4.0 International License.

Characterization of Tween^® Surfactants by MALDI TOF-MS and High Performance Liquid Chromatography in a Ternary Mobile Phase

Abstract

Kurzfassung

References

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