Food Science and Technology Research
Online ISSN : 1881-3984
Print ISSN : 1344-6606
ISSN-L : 1344-6606
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Inactivation of L. fructivorans in Sake Using a Continuous Flow System for High-Pressure Carbonation
Shota TANIMOTO, Hideyuki MATSUMOTO, Kazuyoshi FUJII, Ritsushi OHDOI, Koji SAKAMOTO, Yuichi YAMANE, Masaki MIYAKE, Mitsuya SHIMODA, Yutaka OSAJIMA
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Keywords: high-pressure carbonation, inactivation kinetics of lactic acid bacteria, sake, L. fructivorans, continuous flow system
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2007 Volume 13 Issue 3 Pages 210-214

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Abstract

The inactivation kinetics of lactic acid bacteria, Lactobacillus fructivorans, which is responsible for the deterioration of sake (Japanese rice wine) quality, was investigated using a continuous flow system for high-pressure carbonation. In addition, the effects of ethanol and sugar concentration on lactic acid bacteria inactivation were investigated. Cells of L. fructivorans (1×106CFU/ml) were inactivated at a treatment temperature of 40°C and a pressure of 20MPa, with a residence time of 1.7min. The D-values (decimal reduction time) at 30 and 35°C were 4.3 and 0.6min, respectively. In addition to ethanol, glucose in sake also increased the inactivation efficiency of L. fructivorans by the carbonation treatment. These results suggest that the present technique is an effective microbial inactivation method for alcoholic beverages such as sake.

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© 2007 by Japanese Society for Food Science and Technology
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