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Study on Effects of Ag-SiO2 Core Shell Nanoparticles on Biocompatibility Appraisal of Myoblasts

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Abstract

The main goal of present study was to check interaction of Ag-SiO2 core shell nanoparticles (CSNs) with C2C12 cells. We herein account the synthesis and classification of novel CSNs. We aimed the preparation of CSNs in order to check their biocompatible/cytotoxic impact on C2C12 muscle cells. We aimed to check the CSNs related hazards to human health. The utilized CSNs were synthesized by the straightforward sol–gel method utilizing silver nitrate and tetraethoxysilane as fundamental components. The physicochemical characterization of the CSNs was conceded by means of X-ray diffraction, UV-Vis and transmission electron microscopy. To examine in vitro biocompatibility/cytotoxicity, C2C12 cells were cultured under in vitro environment and afterward subjected to different concentrations of CSNs. The survival of C2C12 cells was evaluated via cell counting Kit-8 assay at exact point gap. These results were authenticated by confocal microscopy. The morphology of C2C12 cells was observed with the help of a phase contrast microscope. In vitro investigation of biological effects of CSNs has revealed that feasibility of cells in culture follows dose (0–20 μg/mL) mode. Overall, results of this investigation demonstrate that synthetic CSNs influence C2C12 cell practicability with time point as well as absorption dependent mode.

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Funding

This research received no Research grant from any funding agency.

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Authors and Affiliations

  1. Department of Biology and Biotechnology, Faculty of Science, Albaha University, 1988, Albaha, Kingdom of Saudi Arabia

    Touseef Amna

  2. Department of Biology, Faculty of Science, Albaha University, 1988, Albaha, Kingdom of Saudi Arabia

    Abdullah A. A. Alghamdi

  3. School of Advanced Materials Engineering and Research Center of Advanced Materials Development, Chonbuk National University, 561-756, Jeonju, South Korea

    Rizwan Khan

  4. Department of Chemistry, Faculty of Science, Albaha University, 1988, Albaha, Kingdom of Saudi Arabia

    M. Shamshi Hassan & Myung-Seob Khil

  5. Department of Organic Materials and Fiber Engineering, Jeonbuk National University, 561-756, Jeonju, Republic of Korea

    M. Shamshi Hassan

Authors
  1. Touseef Amna
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  2. Abdullah A. A. Alghamdi
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  3. Rizwan Khan
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  4. M. Shamshi Hassan
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  5. Myung-Seob Khil
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Correspondence to Touseef Amna or Myung-Seob Khil.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Touseef Amna, Alghamdi, A.A., Khan, R. et al. Study on Effects of Ag-SiO2 Core Shell Nanoparticles on Biocompatibility Appraisal of Myoblasts. Cytol. Genet. 55, 199–204 (2021). https://doi.org/10.3103/S009545272102002X

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