Skip to main content
Log in

Bioinformatics search for plant homologues of Ste20-like serine/threonine protein kinases

  • Published:
Cytology and Genetics Aims and scope Submit manuscript

Abstract

Eleven plant homologuess of animal and yeast STE20-like protein kinases were identified. It was shown that unknown proteins A9RVK0 of the moss Physcomitrella patens ssp. patens and A7P2E2 of the grape Vitis vinifera were the closest plant homologues of STE20-like protein kinases. The Ste20-like protein kinase dst1 of the myxomycete Dictyostelium discoideum was the closest to the plant homologues. The spatial structure of the catalytic domain of the protein A9RVK0 from P. patens ssp. patens was predicted.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Eby, J.J., Holly, S.P., van Drogen, F., Grishin, A.V., Peter, M., Drubin, D.G., and Blumer, K.J., Actin Cytoskeleton Organization Regulated by the PAK Family of Protein Kinases, Curr. Biol., 1998, vol. 8, no. 17, pp. 967–970.

    Article  CAS  PubMed  Google Scholar 

  2. Rohlfs, M., Arasada, R., Batsios, P., Janzen, J., and Schleicher, M., The Ste20-Like Kinase SvkA of Dictyostelium discoideum Is Essential for Late Stages of Cytokinesis, J. Cell Sci., 2007, vol. 120, pp. 4345–4354.

    Article  CAS  PubMed  Google Scholar 

  3. Arasada, R., Son, H., Ramalingam, N., Eichinger, L., Schleicher, M., and Rohlfs, M., Characterization of the Ste20-Like Kinase Krs1 of Dictyostelium discoideum, Eur. J. Cell Biol., 2006, vol. 85, no. 9/10, pp. 1059–1068.

    Article  CAS  PubMed  Google Scholar 

  4. Nichols, C.B., Fraser, J.A., and Heitman, J., PAK Kinases Ste20 and Pak1 Govern Cell Polarity at Different Stages of Mating in Cryptococcus Neoformans, Mol. Biol. Cell., 2004, vol. 15, pp. 4476–4489.

    Article  CAS  PubMed  Google Scholar 

  5. Yamada, E., Tsujikawa, K., Itoh, S., Kameda, Y., Kohama, Y., and Yamamoto, H., Molecular Cloning and Characterization of a Novel Human STE20-Like Kinase, HSLK, Biochem. Biophys. Acta, 2000, vol. 1495, no. 3, pp. 250–262.

    Article  CAS  PubMed  Google Scholar 

  6. Pytowski, B., Hicklin, D.J., Kornhaber, G., Dellaratta, D.V., and Witte, L., Identification and Initial Characterization of MSLK, a Murine Member of the STE20 Family of Kinases, Arch. Biochem. Biophys., 1998, vol. 359, no. 2, pp. 310–319.

    Article  CAS  PubMed  Google Scholar 

  7. Wu, C., Whiteway, M., Thomas, D.Y., and Leberer, E., Molecular Characterization of Ste20p, a Potential Mitogen-Activated Protein Or Extracellular Signal-Regulated Kinase Kinase (MEK) Kinase Kinase from Saccharomyces cerevisiae, J. Biol. Chem., 1995, vol. 270, no. 27, pp. 15984–15992.

    Article  CAS  PubMed  Google Scholar 

  8. Nespoli, A., Vercillo, R., di Nola, L., Diani, L., Giannattasio, M., Plevani, P., and Muzi-Falconi, M., Alk1 and Alk2 Are Two New Cell Cycle-Regulated Haspin-Like Proteins in Budding Yeast, Cell Cycle, 2006, vol. 5, no. 13, pp. 1464–1471.

    CAS  PubMed  Google Scholar 

  9. Sullivan, M. and Uhlmann, F., A Non-Proteolytic Function of Separase Links the Onset of Anaphase to Mitotic Exit, Nat. Cell Biol., 2003, vol. 5, no. 3, pp. 249–254.

    Article  CAS  PubMed  Google Scholar 

  10. Ahn, S.H., Cheung, W.L., Hsu, J.Y., Diaz, R.L., Smith, M.M., and Allis, C.D., Sterile 20 Kinase Phosphorylates Histone H2B at Serine 10 during Hydrogen Peroxide-Induced Apoptosis in S. cerevisiae, Cell, 2005, vol. 120, no. 1, pp. 25–36.

    Article  CAS  PubMed  Google Scholar 

  11. Froquet, R., Cherix, N., Birke, R., Benghezal, M., Cameroni, E., Letourneur, F., Mösch, H.U., De Virgilio, C., and Cosson, P., Control of Cellular Physiology by TM9 Proteins in Yeast and Dictyostelium, J. Biol. Chem., 2008, vol. 283, no. 11, pp. 6764–6772.

    Article  CAS  PubMed  Google Scholar 

  12. Tatebayashi, K., Tanaka, K., Yang, H.Y., Yamamoto, K., Matsushita, Y., Tomida, T., Imai, M., and Saito, H., Trans-Membrane Mucins Hkr1 and Msb2 Are Putative Osmosensors in the SHO1 Branch of Yeast HOG Pathway, EMBO J., 2007, vol. 26, no. 15, pp. 3521–3533.

    Article  CAS  PubMed  Google Scholar 

  13. Moskow, J.J., Gladfelter, A.S., Lamson, R.E., Pryciak, P.M., and Lew, D.J., Role of Cdc42p in Pheromone-Stimulated Signal Transduction in Saccharomyces cerevisiae, Mol. Cell Biol., 2000, vol. 20, no. 20, pp. 7559–7571.

    Article  CAS  PubMed  Google Scholar 

  14. Pan, X. and Heitman, J., Sok2 Regulates Yeast Pseudohyphal Differentiation via a Transcription Factor Cascade That Regulates Cell-Cell Adhesion, Mol. Cell Biol., 2000, vol. 20, no. 22, pp. 8364–8372.

    Article  CAS  PubMed  Google Scholar 

  15. Cullen, P.J., Sabbagh, W., Jr., Graham, E., Irick, M.M., van Olden, E.K., Neal, C., Delrow, J., Bardwell, L., and Sprague, G.F., Jr., A Signaling Mucin at the Head of the Cdc42- and MAPK-Dependent Filamentous Growth Pathway In Yeast, Genes Dev., 2004, vol. 18, no. 14, pp. 1695–1708.

    Article  CAS  PubMed  Google Scholar 

  16. Ni, L. and Snyder, M., A Genomic Study of the Bipolar Bud Site Selection Pattern in Saccharomyces Cerevisiae, Mol. Biol. Cell., 2001, vol. 12, no. 7, pp. 2147–2170.

    CAS  PubMed  Google Scholar 

  17. Burakov, A.V., Zhapparova, O.N., Kovalenko, O.V., Zinovkina, L.A., Potekhina, E.S., Shanina, N.A., Weiss, D.G., Kuznetsov, S.A., and Nadezhdina, E.S., Ste20-Related Protein Kinase LOSK (SLK) Controls Microtubule Radial Array in Interphase, Mol. Biol. Cell, 2008, vol. 19, no. 5, pp. 1952–1961.

    Article  CAS  PubMed  Google Scholar 

  18. Qian, Y.W., Erikson, E., and Maller, J.L., Purification and Cloning of a Protein Kinase That Phosphorylates and Activates the Polo-Like Kinase Plx1, Science, 1998, vol. 282, pp. 1701–1704.

    Article  CAS  PubMed  Google Scholar 

  19. Ellinger-Ziegelbauer, H., Karasuyama, H., Yamada, E., Tsujikawa, K., Todokoro, K., and Nishida, E., Ste20-Like Kinase (SLK), a Regulatory Kinase for Polo-Like Kinase (Plk) during the G2/M Transition in Somatic Cells, Genes Cells, 2000, vol. 5, no. 6, pp. 491–498.

    Article  CAS  PubMed  Google Scholar 

  20. Demonacos, C., Krstic-Demonacos, M., Smith, L., Xu, D., O’Connor, D.P., Jansson, M., and La Thangue, N.B., A New Effector Pathway Links ATM Kinase with the DNA Damage Response, Nat. Cell. Biol., 2004, vol. 6, no. 10, pp. 968–976.

    Article  CAS  PubMed  Google Scholar 

  21. Matsuoka, S., Ballif, B.A., Smogorzewska, A., McDonald, E.R., 3rd, Hurov, K.E., Luo, J., Bakalarski, C.E., Zhao, Z., Solimini, N., Lerenthal, Y., Shiloh, Y., Gygi, S.P., and Elledge, S.J. ATM and ATR Substrate Analysis Reveals Extensive Protein Networks Responsive to DNA Damage, Science, 2007, vol. 316, no. 5828, pp. 1160–1166.

    Article  CAS  PubMed  Google Scholar 

  22. Hao, W., Takano, T., Guillemette, J., Papillon, J., Ren, G., and Cybulsky, A.V., Induction of Apoptosis by the Ste20-Like Kinase SLK, a Germinal Center Kinase That Activates Apoptosis Signal-Regulating Kinase and P38, J. Biol. Chem., 2006, vol. 281, no. 6, pp. 3075–3084.

    Article  CAS  PubMed  Google Scholar 

  23. Leeuw, T., Wu, C., Schrag, J.D., Whiteway, M., Thomas, D.Y., and Leberer, E., Interaction of a G-Protein Beta-Sub-Unit with a Conserved Sequence in Ste20/PAK Family Protein Kinases, Nature, 1998, vol. 391, no. 6663, pp. 191–195.

    Article  CAS  PubMed  Google Scholar 

  24. Potekhina, E.S. and Nadezhdina, E.S., Mitogen-Activated Protein Kinase Cascades and the Involvement of Ste20-Like Protein Kinases in Them, Usp. Biol. Khim., 2002, vol. 42, pp. 235–256.

    CAS  Google Scholar 

  25. Sabourin, L.A. and Rudnicki, M.A., Induction of Apoptosis by SLK, a Ste20-Related Kinase, Oncogene, 1999, vol. 18, no. 52, pp. 7566–7575.

    Article  CAS  PubMed  Google Scholar 

  26. Cvrcková, F., De Virgilio, C., Manser, E., Pringle, J.R., and Nasmyth, K., Ste20-Like Protein Kinases Are Required for Normal Localization of Cell Growth and for Cytokinesis in Budding Yeast, Genes Dev., 1995, vol. 9, pp. 1817–1830.

    Article  PubMed  Google Scholar 

  27. Lee, K.-K. and Yonehara, S., Phosphorylation and Dimerization Regulate Nucleocytoplasmic Shuttling of Mammalian STE20-Like Kinase (MST), J. Biol. Chem., 2002, vol. 277, no. 14, pp. 12351–12358.

    Article  CAS  PubMed  Google Scholar 

  28. Winge, P., Brembu, T., and Bones, A.M., Cloning of a Arabidopsis thaliana Serine/Threonine Kinase with Homology to Yeast STE20 and Mammalian Stress Activated Kinases. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases: GI: 2352083. http://www.ncbi.nlm.nih.gov/nuccore/2352083.

  29. Karpov, P.A., Nadezhdina, E.S., Yemets, A.I., Matusov, V.G., Nyporko, A.Yu., Shashina, N.Yu., and Blume, Ya.B., Bioinformational Search for Plant Protein Kinases Involved in the Formation of Microtubular Proteins and Regulation of Cell Division, Tsitol. Genet., 2009, vol. 43, no. 3, pp. 63–79.

    CAS  Google Scholar 

  30. The UniProt Consortium. The Universal Protein Resource (UniProt), Nucl. Acids Res., 2008, vol. 36, pp. D190–195.

    Article  Google Scholar 

  31. Korf, I., Yandell, M., and Bedell, J., BLAST, Sebastopol: O’Reilly Associates, 2003.

    Google Scholar 

  32. Claverie, J.-M. and Notredame, C., Bioinformatics for Dummies, New York: Wiley Publ., 2007.

    Google Scholar 

  33. Letunic, I., Copley, R.R., Pils, B., Pinkert, S., Schultz, J., and Bork, P., SMART 5: Domains in the Context of Genomes and Networks, Nucleic Acids Res., 2006, vol. 34, pp. D257–260.

    Article  CAS  PubMed  Google Scholar 

  34. Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J., and Higgins, D.G., Clustal Wand Clustal X Version 2.0, Bioinformatics, 2007, vol. 23, pp. 2947–2948.

    Article  CAS  PubMed  Google Scholar 

  35. Zhang, C., Brankle, S., Mallery, E., and Szymanski, D., Composition and Function of the Arabidopsis WAVE Complex during Epidermal Morphogenesis, 2005, p. 131.

  36. Hanks, S.K. and Quinn, A.M., Protein Kinase Catalytic Domain Sequence Database: Identification of Conserved Features of Primary Structure and Classification of Family Members, Methods Enzymol., 1991, vol. 200, pp. 38–62.

    Article  CAS  PubMed  Google Scholar 

  37. Nei, M. and Kumar, S., Molecular Evolution and Phylogenetics, New York: Oxford Univ. Press, 2000.

    Google Scholar 

  38. Atteson, K., in Lecture Notes in Computer Science, Jiang, T. and Lee, D., Eds., Berlin: Springer, 1997, vol. 127, pp. 101–110.

    Google Scholar 

  39. Page, R.D.M., TREEVIEW: An Application to Display Phylogenetic Trees on Personal Computers, Comp. Appl. Biosci., 1996, vol. 12, pp. 357–358.

    CAS  PubMed  Google Scholar 

  40. Kumar, S., Dudley, J., Nei, M., and Tamura, K., MEGA: A Biologist-Centric Software for Evolutionary Analysis of DNA and Protein Sequences, Brief. Bioinform., 2008, vol. 9, pp. 299–306.

    Article  CAS  PubMed  Google Scholar 

  41. Krieger, E., Nabuurs, S.B., and Vriend, G., Homology Modeling in Structural Bioinformatics, Bourne, P.E. and Weissig, H., Eds., Hoboken: Wiley, 2003, pp. 509–524.

    Google Scholar 

  42. Berman, H.M., Westbrook, J., Feng, Z., Gilliland, G., Bhat, T.N., Weissig, H., Shindyalov, I.N., and Bourne, P.E., The Protein Data Bank, Nucleic Acids Res., 2000, vol. 28, pp. 235–242.

    Article  CAS  PubMed  Google Scholar 

  43. Das, B., Meirovitch, H., and Navon, I.M., Performance of Hybrid Methods for Large-Scale Unconstrained Optimization As Applied to Models of Proteins, J. Comput. Chem., 2003, vol. 24, pp. 1222–12231.

    Article  CAS  PubMed  Google Scholar 

  44. Guex, N. and Peitsch, M.C., SWISS-MODEL and the Swiss-PdbViewer: An Environment for Comparative Protein Modeling, Electrophoresis, 1997, vol. 18, pp. 2714–2723.

    Article  CAS  PubMed  Google Scholar 

  45. Dan, I., Watanabe, N.M., and Kusumi, A., The Ste20 Group Kinases As Regulators of MAP Kinase Cascades, Trends Cell Biol., 2001, vol. 11, no. 5, pp. 220–230.

    Article  CAS  PubMed  Google Scholar 

  46. Creasy, C.L., Ambrose, D.M., and Chernoff, J., The Ste20-Like Protein Kinase, Mst1, Dimerizes and Contains An Inhibitory Domain, J. Biol. Chem., 1996, vol. 271, no. 35, pp. 21049–21053.

    Article  CAS  PubMed  Google Scholar 

  47. Krieger, E., Nabuurs, S.B., and Vriend, G., Homology Modeling, Chapter 25, in Structural Bioinformatics, vol. 44, Bourne, P.E. and Weissig, H., Eds., New York: Wiley, 2003, pp. 509–523.

    Chapter  Google Scholar 

  48. Strange, K. and Denton, J., Ste20-Type Kinases: Evolutionarily Conserved Regulators of Ion Transport and Cell Volume, Physiology, 2006, vol. 21, no. 1, pp. 61–68.

    Article  CAS  PubMed  Google Scholar 

  49. Rohlfs, M., Arasada, R., Batsios, P., Janzen, J., and Schleicher, M., The Ste20-Like Kinase SvkA of Dictyostelium discoideum Is Essential for Late Stages of Cytokinesis, J. Cell Sci., 2007, vol. 120, pp. 4345–4354.

    Article  CAS  PubMed  Google Scholar 

  50. Xu, X., Jiao, X., and Jin, T., Spectral Confocal FRET Imaging Reveals the Spatial-Temporal Activation of the Heterotrimeric G-Proteins in Single Chemotactic Cells, in Abstracts of International Dictyostelium Conference, Lorne, Victoria, Australia: Erskine House, 2003, p. 6.

    Google Scholar 

  51. Goldberg, J.M., Manning, G., Liu, A., Fey, P., Pilcher, K.E., Xu, Y., and Smith, J.L., The Dictyostelium Kinome— Analysis of the Protein Kinases from a Simple Model Organism, PLoS Genet., 2006, vol. 2, no. 3, p. 2.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to P. A. Karpov.

Additional information

Original Russian Text © P.A. Karpov, A.I. Emets, V.G. Matusov, A.Yu. Nyporko, E.S. Nadezhdina, Ya.B. Blume, 2009, published in Tsitologiya i Genetika, 2009, Vol. 43, No. 6, pp. 68–77.

About this article

Cite this article

Karpov, P.A., Emets, A.I., Matusov, V.G. et al. Bioinformatics search for plant homologues of Ste20-like serine/threonine protein kinases. Cytol. Genet. 43, 419–428 (2009). https://doi.org/10.3103/S0095452709060097

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3103/S0095452709060097

Keywords

Navigation