Abstract
The universal cell mediator nitric oxide (NO) mediating the physiological effects via so-called NO donor compounds prolonging its life time. The concentration of NO metabolites in the pancreatic juice of chicken was determined by the enzymatic sensor. It was found that pancreatic juice from starved birds contains nitrate and no other nitro- or nitroso compounds. After feeding, the NO donors appear in pancreatic juice in concentrations of a few tens of μM. These compounds were present in pancreatic juice for several hours after feeding. It is possible that NO donors promote relaxation of the smooth muscles in the walls of pancreatic ducts. Concentration of NO donors in blood serum also increased 2.5–3.0-fold after feeding and remained high during a few subsequent hours. The preliminary subcutaneous administration of atropine to the birds prevent this increase with no effects on the initial concentrations of NO donors in serum. This suggests that the increase is associated with cholinergic stimulation and, therefore, with the activity of the parasympathetic nervous system. The concentration of trypsin in serum also increased after feeding, and atropine also prevents this increase. It is known that serum trypsin acts as a regulator of pancreatic function. Intravenous administration of trypsin led to an insignificant increase of tryptic activity in serum but induced the increase of NO donor concentration twofold. Apparently, nitric oxide, represented by these compounds, can act as a humoral factor in the regulation of pancreatic function in pancreatic juice and possibly in the blood.
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References
Moncada, S., Palmer, R., and Higgs, E., Nitric oxide: Physiology, pathophysiology, and pharmacology, Pharm. Rev., 1991, vol. 43, no. 2, pp. 109–142.
Vanin, A., Dinitrosyl iron complexes with thiolate ligands: Physico-chemistry, biochemistry and physiology, Nitric Oxide, 2009, vol. 21, pp. 1–13.
Tarpey, M., Wink, D., and Grisham, M., Methods for detection of reactive metabolites of oxygen and nitrogen: In vitro and in vivo considerations, Am. J. Physiol. Regul. Integr. Comp. Physiol., 2004, vol. 286, pp. 431–444.
Titov, V.Y., Kosenko, O.V., Starkova, E.S., Kondratov, G.V., Borkhunova, E.N., Petrov, V.A., and Osipov, A.N., Enzymatic sensor detects some forms of nitric oxide donors undetectable by other methods in living tissues, Bull. Exp. Biol. Med., 2016, vol. 162, no. 1, pp. 107–110.
Jyotheeswaran, S., Li, P., Chang, T., and Chey, W., Endogenous nitric oxide mediates pancreatic exocrine secretion stimulated by secretin and cholecystokinin in rats, Pancreas, 2000, vol. 20, no. 4, pp. 401–407.
DiMagno, M., Hao, Y., Tsunoda, Y., Williams, J., and Owyang, C., Secretagogue-stimulated pancreatic secretion is differentially regulated by constitutive NOS isoforms in mice, Am. J. Physiol. Gastrointest. Liver Physiol., 2004, vol. 286, no. 3, pp. 428–436.
Onaga, T., Nagashima, C., and Sakata, T., Effect of nitric oxide synthase inhibitors on the temporal coordination of duodenal contractions and pancreatic exocrine secretion in sheep, J. Comp. Physiol., 2000, vol. 170, nos. 5–6, pp. 469–479.
Titov, V.Yu., The enzymatic technologies open new possibilities for studying nitric oxide (NO) metabolism in living systems, Curr. Enzyme Inhib., 2011, vol. 7, no. 1, pp. 56–70.
Fisinin, V.I., Egorov, I.A., and Lenkova, T.N., Rukovodstvo po optimizatsii retseptov kombikormov dlya sel’skokhozyaistvennoi ptitsy (Guidelines for Optimizing Compound Feed Recipes for Poultry), Sergiev Posad, 2014, pp. 3–4.
Vertiprakhov, V. and Egorov, I., The influence of feed intake and conditioned reflex on exocrine pancreatic function in broiler chicks, Open J. Anim. Sci., 2016, vol. 6, pp. 298–303.
Vertiprakhov, V.G., Features of the secretory function of the pancreas of broiler chickens and the possibilities of correcting the digestion of animals by zeolite-based enzyme preparations, Doctoral (Biol.) Dissertation, Novosibirsk, 2004, pp. 59–60.
Mikhailova, A.G., Khairullin, R.F., Rumsh, L.D., Demidyuk, I.V., Kostrov, S.V., Grinberg, N.V., Burova, T.V., and Grinberg, V.Y., Cloning, sequencing, expression, and characterization of thermostability of oligopeptidase B from Serratia proteamaculans, a novel psychrophilic protease, Protein Expression Purif., 2014, vol. 93, pp. 63–76. doi 10.1016/j.pep.2013.10.011
Laporte, J. and Tremolieres, J., Regulation hormonale de la sécrétion enzymatique du pancréas exocrine, C. R. Acad. Sci., Ser. D, 1971, vol. 273, pp. 1205–1207.
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Original Russian Text © V.Yu. Titov, V.G. Vertiprakhov, A.S. Ushakov, V.I. Fisinin, I.I. Kochish, V.A. Petrov, 2018, published in Rossiiskaya Sel’skokhozyaistvennaya Nauka, 2018, No. 5, pp. 57–60.
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Titov, V.Y., Vertiprakhov, V.G., Ushakov, A.S. et al. The Role of Nitric Oxide in the Exocrine Pancreatic Function in Chicken. Russ. Agricult. Sci. 44, 559–562 (2018). https://doi.org/10.3103/S1068367418060174
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DOI: https://doi.org/10.3103/S1068367418060174