Abstract
Gibberellin signaling pathway genes encoding the DELLA protein and gibberellin receptor GID1 were sequenced in several varieties (Alpha, Valdai, Orlovskaya 9, Pracha) and one line of rye (EM-1) using next generation methods. The revealed multiple alleles of these genes differ mainly in single-nucleotide polymorphisms and less frequently in insertions and deletions. Most of the detected mutations turned out to be synonymous or located in the noncoding regions of the genes. Changes in the amino acid sequences of proteins associated with other mutations are probably functionally neutral. Mutations similar to wheat reduced-height gibberellin-insensitive alleles were not detected.
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ACKNOWLEDGMENTS
We thank Dr. I.G. Loskutov, Doctor of Science, Head of the Department of Genetic Resources of Oat, Rye, and Barley of the Vavilov All-Russia Institute for Plant Genetic Resources; Dr. V.D. Kobylyansky, Doctor of Science, Honorary Professor of Vavilov All-Russia Institute for Plant Genetic Resources; and Dr. V.S. Rubetz, Doctor of Science, Professor of the Department of Genetics, Plant Breeding, and Seed Farming of the Russian State Agrarian University–Moscow Timiryazev Agricultural Academy for kindly providing plant material.
Funding
This work was supported by the Russian Scientific Foundation, project no. 17-76-20023.
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Translated by Mikhail Bibov
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Bazhenov, M.S., Chernook, A.G. & Divashuk, M.G. Molecular Analysis of the Gibberellin Signaling Pathway Genes in Cultivated Rye (Secale cereale L.). Moscow Univ. Biol.Sci. Bull. 75, 125–129 (2020). https://doi.org/10.3103/S0096392520030025
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DOI: https://doi.org/10.3103/S0096392520030025