Abstract
A cross was made of Elymus repens onto the wheat cultivar Crocus and BC1 progeny advanced to BC1F7 by single seed descent. Sixteen lines were selected based on agronomic performance and evaluated in an FHB epiphytotic nursery. Eight lines with FHB resistance were selected. Based on GISH analysis, line PI 142-3-1-5 had 42 chromosomes with one pair of chromosomes showing telomeric translocations on both arms. This chromosome was identified as 3D by using SSR markers. An evaluation of lines with single translocations revealed that FHB resistance was contributed by the translocation on the long arm of chromosome 3D. That line has minimal linkage drag and should be amenable to applications inbreeding for disease resistance.
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References
Ban, T., Evaluation of resistance to fusarium head blight in indigenous Japanese species of Agropyron (Elymus), Euphytica, 1997, vol. 97, pp. 39–44.
Buerstmayr, H., Ban, T., and Anderson, J.A., QTL mapping and marker assisted selection for Fusarium head blight in wheat: a review, Plant Breed., 2009, vol. 128, pp. 1–26.
Cai, X., Chen, P.D., Xu, S.S., Oliver, R.E., and Chen, X., Utilization of alien genes to enhance Fusarium head blight resistance in wheat: a review, Euphytica, 2005, vol. 142, pp. 309–318.
Chen, P.D., Liu, W.X., Yuan, J.H., et al., Development and characterization of wheat–Elymus racemosus translocation lines with resistance to Fusarium head blight, Theor. Appl. Genet., 2005, vol. 111, pp. 941–948.
Dewey, D.R., Cytogenetics of polyhaploid Agropyron repens, Torrey Bot. Club, 1974, vol. 101, pp. 266–271.
Fedak, G., Cao, W., Xue, A., Savard, M.E., Clark, J., and Somers, D.J., Enhancement of Fusarium head blight resistance in bread wheat and durum by means of wide crosses, in Wheat Conference, November 27–December 02, 2005, Mar del Plata, Argentina, Buck, H.T., Nisi, J.E., and Salomon, N., Eds., 2005, pp. 91–95.
Fedak, G., Alien introgressions from wild Triticum species, T. monococcum, T. urartu, T. turgidum, T. dicoccum, T. dicoccoides, T. carthlicum, T. araraticum, T. timopheevii, and T. miguschovae, in Alien Introgression in Wheat: Cytogenetics, Molecular Biology and Genomics, Molnar, M., Dolezel, J., and Ceoloni, C., Eds., Springer, 2015, chapter 8, pp. 191–219.
Fedak, G., Cao, W., Chi, D., Somers, D.T., Miller, S.S., Ouellet, T., Xue, A., Gilbert, J., Savard, M.E., and Voldeng, H., New sources of resistance to Fusarium head blight and their modes of action, in Proceedings of the 2011 National Fusarium Head Blight Forum, Canty, S., Clarke, A., Anderson-Scully, A., and Sanford, D., Eds., 2011, pp. 19–22.
Fedak, G., Comeau, A., and St-Pierre, C.A., Meiosis in Triticum aestivum x Elytrigia repens hybrids, Can. J. Genet. Cytol., 1986, vol. 27, pp. 430–432.
Han, F.P., Fedak, G., Benabdelmouna, A., Armstrong, K.C., and Ouellet, T., Characterization of six wheat x Thinopyrum intermedium derivatives by GISH, RFLP and multicolor GISH, Genome, 2003, vol. 46, pp. 490–495.
Jauhar, P.P. and Peterson, T.S., Synthesis and characterization of advanced durum wheat hybrids and addition lines with Thinopyrum chromosomes, J. Hered., 2013, vol. 104, pp. 428–436.
Miller, S.S., Watson, E., Lazebnik, J., Gulden, S., Balcerzak, M., Fedak, G., and Ouellet, T., Characterization of an alien source of resistance to Fusarium head blight transferred to Chinese spring wheat, Botany, 2011, vol. 89, pp. 301–311.
Oliver, R.E., Cai, X., Xu, S.S., Chen, X., and Stack, R.W., Wheat-alien species derivatives: a novel source of resistance to Fusarium head blight in wheat, Crop Sci., 2005, vol. 45, pp. 1353–1360.
Oliver, R.E., Stack, R.W., Miller, J.D., and Cai, X., Reaction of wild emmer wheat accessions to Fusarium head blight, Crop Sci., 2007, vol. 47, pp. 893–899.
Shen, X.R. and Ohm, H., Molecular mapping of Thinopyrum-derived Fusarium head blight resistance in common wheat, Mol. Breed., 2007, vol. 20, pp. 131–140.
Shen, X., Kong, L.R., and Ohm, H., Fusarium head blight resistance in hexaploid wheat (Triticum aestivum)-Lophopyrum genetic lines and tagging of alien chromatin by PCR markers, Theor. Apply. Genet., 2008, vol. 108, pp. 808–813.
Somers, D.J., Isaac, P., and Edwards, K., A high density microsatellite consensus map for bread wheat (Triticum aestivum), Theor. Apply. Genet., 2004, vol.109.
Somers, D.J., Fedak, G., Clarke, J., and Cao, W., Mapping of fhb resistance QTLs in tetraploid wheat, Genome, 2006, vol. 49, pp. 1586–1593.
Steed, A.F., Chandler, M., Thomsett, N., Faure, S., and Nicholson, P., Identification of type I resistance to Fusarium head blight controlled by a major gene located on chromosome 4A of Triticum macha, Theor. Apply. Genet., 2005, vol. 111, pp. 521–529.
Zeng, J., Cao, W., Hucl, P., Yang, Y., Xue, A.G., Chi, D., and Fedak, G., Molecular cytogenetic analysis of wheat–Elymus repens introgression lines with resistance to Fusarium head blight, Genome, 2015, vol. 56, pp. 75–82.
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Published in Ukrainian in Tsitologiya i Genetika, 2017, Vol. 51, No. 2, pp. 68–72.
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Fedak, G., Cao, W., Wolfe, D. et al. Molecular characterization of Fusarium resistance from Elymus repens introgressed into bread wheat. Cytol. Genet. 51, 130–133 (2017). https://doi.org/10.3103/S0095452717020025
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DOI: https://doi.org/10.3103/S0095452717020025