In order to solve the seeding problem of vegetable plug seedling production, a simple type automatic vegetable seeding machine was previously developed by the station. However, the seeding mechanism of the automatic seeding machine was improved again in this study for the purpose of increasing its versatility and seeding accuracy. The seeding mechanism was improved by employing the one-tray type pop-up seeding mechanics. The up-and-down frequency of pop-up seeding mechanics can be modified in compliance with the necessity of the type of vegetables. The results of seeding tests with Chinensis Petsai showed that the seeding accuracy on 128 cell tray could reach 90.55 %, but the miss seeding rate was only 9.45 % and the seeding speed was about 350 trays per hour. This automatic seeding machine was not only useful for plug seedling production, but also very practical for growing leaf vegetables on seedling trays.