Abstract: The study was carried out at the field dodder (Cuscuta spp.) in Khartoum and Gezira states, to study variation between 28 samples of it in two States using DNA Markers (RAPD). Four primers were used (OPAL15, OPAL18, OPB17 and OPAL20) to amplify the genomic DNA of 28 samples of Cuscuta spp. Two primers (OPB17, OP AL20) showed high percentage (100%) to amplify the genomic DNA polymorphism bands, while the other two primers (OPAL15, OPAL18) were produced (91.7%) polymorphism bands. UPGA analysis showed RAPD Distance Matrix range (0.10-0.98) which reflected high genetic diversity.