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HomeEvent-specific method for the quantification of soybean DAS-68416-4 using real-time PCR
Validation report
Event-specific method for the quantification of soybean DAS-68416-4 using real-time PCR
Validation report
Publication metadata
In line with its mandate the European Union Reference Laboratory for GM Food and Feed (EU RL GMFF), in collaboration with the European Network of GMO Laboratories (ENGL), has validated an event-specific polymerase chain reaction (PCR) method for detecting and quantifying soybean event DAS-68416-4 (unique identifier DAS-68416-4). The validation study was conducted according to the EU-RL GMFF validation procedure (http://gmo-crl.jrc.ec.europa.eu/guidancedocs.htm) and internationally accepted guidelines. In accordance with current EU legislation , Dow AgroSciences LLC provided the detection method and the positive and negative control samples (genomic DNA extracted from soybean kernels harbouring the DAS-68416-4 event as positive control DNA, genomic DNA extracted from conventional soybean kernels as negative control DNA). The EU-RL GMFF prepared the validation samples (calibration samples and blind samples at different GM percentage [DNA/DNA]), organised an international collaborative study and analysed the results. The study confirms that the method meets the method performance requirements as established by the EU-RL GMFF and the ENGL and according to Annex I-2.C.2 to Regulation (EC) No 641/2004 and it fulfils the analytical requirements of Regulation (EU) No 619/2011 .
- Published:
- Corporate author(s): Institute for Health and Consumer Protection (Joint Research Centre)
- Personal author(s): Mazzara, Marco ; Kreysa, Joachim ; Savini, Christian ; Sacco, Maria Grazia
- Themes: Food technology and food safety
- Subject: animal nutrition , DNA , evaluation method , genetically modified organism , human nutrition , quantitative analysis , research method , research report , seed , soya bean
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| ISSN 1831-9424 | ISBN 978-92-79-38870-5 | DOI 10.2788/87396 | Catalogue number LB-NA-26706-EN-N |
- Released on EU Publications:
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