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HomeReport on the verification of the performance of a testing strategy for the detection of wheat MON71800 event using real-time PCR
Report on the verification of the performance of a testing strategy for the detection of wheat MON71800 event using real-time PCR
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In response to a request of DG SANCO to provide National Reference Laboratories (NRLs) as soon as possible with a method to test soft white wheat consignments for the presence of unauthorised GM glyphosate-resistant wheat harbouring the event MON71800, the European Union Reference Laboratory for Genetically Modified Food and Feed (EU-RL GMFF) developed, in collaboration with the European Network of GMO Laboratories (ENGL), a testing strategy intended to be immediately implementable by EU NRLs. The testing strategy is based on a combination of three validated screening methods that allow excluding (detectable) presence of Monsanto’s GM glyphosate-resistant wheat (MON71800) in wheat grain or food/feed products and confirming its presence whenever other GMOs can be excluded. The present report describes the results of the tests carried out by the EU-RL GMFF to verify the testing strategy proposed; the tests were conducted using the positive control sample represented by a crude DNA lysate of MON71800 provided by Monsanto and genomic DNA samples of genetically modified organisms harbouring the CTP2-CP4epsps element for which a validated event-specific method is available.
- Published:
- Corporate author(s): European Union Reference Laboratory for GM Food and Feed (EURL-GMFF) , Institute for Health and Consumer Protection (Joint Research Centre)
- Personal author(s): Patak, Alex ; Savini, Christian ; Kreysa, Joachim ; Nardini, Elena ; Mazzara, Marco ; Angers, Alexandre ; Petrillo, Mauro ; Lievens, Antoon
- Themes: Food technology and food safety
- Subject: genetic engineering , genetically modified organism , metrology , research report , transgenic plant , wheat
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| ISSN 1831-9424 | ISBN 978-92-79-32497-0 | DOI 10.2788/86096 | Catalogue number LB-NA-26070-EN-N |
- Released on EU Publications:
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