Using the electron spin resonance spin trap method, we evaluated the ability of carotenoids (lycopene, β-carotene) in tomato juice to scavenge hydroxyl radicals, alkoxy radicals, superoxide radicals, and singlet oxygen. The lycopene content in tomato juice highly contributed to its hydroxyl radical- and singlet oxygen-scavenging ability. This tendency was found to be particularly notable in dark-red tomato juice with high lycopene content. The contribution of β-carotene, whose content is high in yellow tomato juice, to the radical-scavenging ability was higher than that found in juice from tomatoes of other colors. The reactive oxygen species-scavenging ability of tomato juice was variable, suggesting that the scavenging ability depended on the types of carotenoids contained in the tomato.