THE APPLICATION OF SIMPLE PAPER CHROMATOGRAPHY TO THE STUDY OF GLUTATHIONE IN THE RAT EPIDERMIS
1971 Volume 18 Issue 4 Pages 177-181
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1971 Volume 18 Issue 4 Pages 177-181
The use of paper chromatography has been demonstrated to be a simple and efficient means for the detection and separation of glutathione from the constituent amino acids and the related metabolite. The use of Nethylmalemide to stabilize the sulfhydryl group during chromatography was found to be a marked advance. This technic was applied to the study of glutathione in the epidermis. The incorporation of 14C-glycine into glutathione was demonstrated under the homogenate of rat epidermis.
