Thirteen SSR (simple sequence repeat) markers were successfully developed in carnation using an enriched genomic library method. All 13 primer pairs designed could successfully amplify target fragments of the expected size for the original variety ‘Francesco’ or ‘Barbara’. Thirty-nine of 41 carnation varieties, except for mutant varieties, were successfully distinguishable using 32 putative alleles and 15 polymorphic bands produced from 13 SSR markers. ‘U Conn Sim’ and ‘White Sim’, which are bud mutants originated from ‘William Sim’, showed an identical genotype to ‘William Sim’. Three (CB026a, CB057a, and CB060a) of 13 SSR markers were thought to be derived from multi-locus because the 3 SSR markers produced more than 3 discrete amplified fragments. The number of alleles per locus ranged from 1 (CB020a and CB041a) to 9 (CB003a), with an average value of 3.2. The values of the observed heterozygosity (H_O) and the expected heterozygosity (H_E) ranged from 0 to 0.59 (mean 0.30) and 0 to 0.68 (mean 0.34), respectively. The power of discrimination (PD) ranged from 0 (CB020a and CB041a) to 0.93 (CB026a) (mean 0.49). The parentage of 2 carnation varieties from intraspecific crosses was analyzed using 10 SSR markers, and the hybridity of the progenic varieties was confirmed. SSR markers were very effective for variety discrimination and determination of carnation parentage.