Expression of TLR2 and TLR4 on peripheral blood monocytes during exacerbation of atopic dermatitis
Background:
In atopic dermatitis (AD), monocytes, which accumulate in the inflamed skin, are characterized by a significantly impaired Toll-like receptors (TLR) expression and TLR2-mediated cytokine secretion. However, data on expression of TLR on monocytes of peripheral blood (PB) in AD are not available.
Objective:
To investigate TLR2 and TLR4 expression on PB monocytes during AD exacerbation and to assess the relationships between TLR expressions with AD clinical severity and with serum interleukin (IL) 4, IL-10, and IL-17a levels.
Methods:
The objective Scoring Atopic Dermatitis index, TLR2 and TLR4 expression on CD14+ human leukocyte antigen-DR (HLA-DR+) PB monocytes by flow cytometry, serum IL-4, IL-10, IL-17a (enzyme-linked immunosorbent assay) and total immunoglobulin E levels were measured at study entry and after 4 months in patients with AD and healthy controls.
Results:
Eighty-two patients with AD, 35 women (45.1%) and 47 men (54.9%), mean (standard deviation [SD]) age, 42.2 ± 11.5 years, were included. Thirty healthy volunteers served as controls. We observed a significant difference in the levels of TLR2 expression in the CD14+ HLA-DR+ PB monocytes of patients with AD (mean [SD], 51.6 ± 23.1% and 264 ± 118 cells/mm3) at exacerbation (but not at the end of the 4-month postexacerbation period) compared with the healthy control subjects (mean [SD], 22.3 ± 10.6% and 105 ± 50 cells/mm3; p < 0.001). TLR4 expression in PB monocytes was significantly greater in AD (mean [SD], 50.1 ± 20.9% and 275 ± 114 cells/mm3) than in the healthy subjects (mean [SD], 31.2 ± 8.7% and 147 ± 41 cells/mm3; p < 0.001) both at exacerbation and at the 4-month postexacerbation period. Significant correlations between TLR2+ (but not TLR4+) PB monocytes and the objective Scoring Atopic Dermatitis index (r = 0.604, p < 0.001), serum levels of IL-17a and TLR2+ PB monocytes (r = 0.416, p = 0.027), and IL-4 and TLR2+ PB monocytes (r = −0.307, p = 0.014) were observed during AD exacerbation.
Conclusion:
PB CD14+ HLA-DR+ TLR2+ monocytes might have a role in the skewing of a T-helper 2/T-helper 17‐mediated immune response during AD flare.
In atopic dermatitis (AD), monocytes, which accumulate in the inflamed skin, are characterized by a significantly impaired Toll-like receptors (TLR) expression and TLR2-mediated cytokine secretion. However, data on expression of TLR on monocytes of peripheral blood (PB) in AD are not available.
Objective:
To investigate TLR2 and TLR4 expression on PB monocytes during AD exacerbation and to assess the relationships between TLR expressions with AD clinical severity and with serum interleukin (IL) 4, IL-10, and IL-17a levels.
Methods:
The objective Scoring Atopic Dermatitis index, TLR2 and TLR4 expression on CD14+ human leukocyte antigen-DR (HLA-DR+) PB monocytes by flow cytometry, serum IL-4, IL-10, IL-17a (enzyme-linked immunosorbent assay) and total immunoglobulin E levels were measured at study entry and after 4 months in patients with AD and healthy controls.
Results:
Eighty-two patients with AD, 35 women (45.1%) and 47 men (54.9%), mean (standard deviation [SD]) age, 42.2 ± 11.5 years, were included. Thirty healthy volunteers served as controls. We observed a significant difference in the levels of TLR2 expression in the CD14+ HLA-DR+ PB monocytes of patients with AD (mean [SD], 51.6 ± 23.1% and 264 ± 118 cells/mm3) at exacerbation (but not at the end of the 4-month postexacerbation period) compared with the healthy control subjects (mean [SD], 22.3 ± 10.6% and 105 ± 50 cells/mm3; p < 0.001). TLR4 expression in PB monocytes was significantly greater in AD (mean [SD], 50.1 ± 20.9% and 275 ± 114 cells/mm3) than in the healthy subjects (mean [SD], 31.2 ± 8.7% and 147 ± 41 cells/mm3; p < 0.001) both at exacerbation and at the 4-month postexacerbation period. Significant correlations between TLR2+ (but not TLR4+) PB monocytes and the objective Scoring Atopic Dermatitis index (r = 0.604, p < 0.001), serum levels of IL-17a and TLR2+ PB monocytes (r = 0.416, p = 0.027), and IL-4 and TLR2+ PB monocytes (r = −0.307, p = 0.014) were observed during AD exacerbation.
Conclusion:
PB CD14+ HLA-DR+ TLR2+ monocytes might have a role in the skewing of a T-helper 2/T-helper 17‐mediated immune response during AD flare.
Keywords: IL-17; SCORAD; TLR2; TLR4; atopic; dermatitis; monocytes
Document Type: Research Article
Affiliations: Pathophysiology Department, Chita Medical Academy, Chita, Russia
Publication date: 01 November 2015
- Allergy and Asthma Proceedings is a peer reviewed publication dedicated to distributing timely scientific research regarding advancements in the knowledge and practice of allergy, asthma and immunology. Its primary readership consists of allergists and pulmonologists.
The goal of the Proceedings is to publish articles with a predominantly clinical focus which directly impact quality of care for patients with allergic disease and asthma and by having the potential to directly impact the quality of patient care. AAP welcomes the submission of original works including peer-reviewed original research and clinical trial results. Additionally, as the official journal of the Eastern Allergy Conference (EAC), AAP will publish content from EAC poster sessions as well as review articles derived from EAC lectures.
Featured topics include asthma, rhinitis, sinusitis, food allergies, allergic skin diseases, diagnostic techniques, allergens, and treatment modalities. Published material includes peer-reviewed original research, clinical trials and review articles.
Articles marked "F" offer free full text for personal noncommercial use only.
The journal is indexed in Thomson Reuters Web of Science and Science Citation Index Expanded, plus the National Library of Medicine's PubMed service. - Editorial Board
- Information for Authors
- Submit a Paper
- Information for Advertisers
- Reprint Requests
- Commercial level: Permission to use content
- www.JFoodAllergy.com
- Ingenta Connect is not responsible for the content or availability of external websites
Receive new issue alert- Access Key
- Free content
- Partial Free content
- New content
- Open access content
- Partial Open access content
- Subscribed content
- Partial Subscribed content
- Free trial content