Geometry of artificial ECM. Three-Dimensional Structure of Titanium-Web (TW) Promotes Differentiation of Human Bone Marrow Mesenchymal Cells into Osteoblasts

Dong Li; Shouhei Iku; Kazuki Nemoto; Yasuo Kokai; Tetsuya Odajima; Ryota Yoshimoto; Tohru Kaku; Hideki Katoh; Hiroyuki Shiota; Yasuo Seki; Naomi Ogura; Yoshimitsu Abiko; Yoshinori Kuboki

doi:10.2485/jhtb.14.333

International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005

Geometry of artificial ECM. Three-Dimensional Structure of Titanium-Web (TW) Promotes Differentiation of Human Bone Marrow Mesenchymal Cells into Osteoblasts

Dong Li, Shouhei Iku, Kazuki Nemoto, Yasuo Kokai, Tetsuya Odajima, Ryota Yoshimoto, Tohru Kaku, Hideki Katoh, Hiroyuki Shiota, Yasuo Seki, Naomi Ogura, Yoshimitsu Abiko, Yoshinori Kuboki

Author information

Dong Li
School of Medicine, Sapporo Medical University
Shouhei Iku
Koken Bioscience Institute
Kazuki Nemoto
Koken Bioscience Institute
Yasuo Kokai
School of Medicine, Sapporo Medical University
Tetsuya Odajima
School of Medicine, Sapporo Medical University
Ryota Yoshimoto
School of Dentistry, Health Sciences University of Hokkaido
Tohru Kaku
School of Dentistry, Health Sciences University of Hokkaido
Hideki Katoh
School of Dentistry, Health Sciences University of Hokkaido
Hiroyuki Shiota
Medical Device Group, Nippon Cable System Inc.
Yasuo Seki
Medical Device Group, Nippon Cable System Inc.
Naomi Ogura
Nihon University School of Dentistry at Matsudo
Yoshimitsu Abiko
Nihon University School of Dentistry at Matsudo
Yoshinori Kuboki
School of Medicine, Sapporo Medical University
Koken Bioscience Institute
Graduate School of Dental Medicine, Hokkaido University

Keywords: three-dimensional culture, scaffold, titanium fibers, bone marrow mesenchymal cells, stem cells

JOURNAL FREE ACCESS

2005 Volume 14 Issue 2 Pages 333-334

DOI https://doi.org/10.2485/jhtb.14.333

Details

Abstract

We demonstrate that a new culture system using a titanium web (TW) consisted of titanium fibers (50 mm diameter) can promote differentiation of mesenchymal cells into osteoblasts without conventional application of dexamethasone. Human mesenchymal stem cells (hMSC, Cambrex) and MC3T3-E1 were cultured on titanium web (87% of porosity). ALP/DNA in hMSC at 2 weeks was 2 times higher and MC3T3-E1 at 4 weeks was 4 times higher on TW than those on the conventional plastic dishes. Effect of 3D structure of TW is self-evident and the results will open a new age of 3D bone cell biology using TW.

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