J Appl Biomed 9:103-109, 2011 | DOI: 10.2478/v10136-009-0032-6

Square wave voltammetry on screen printed electrodes: comparison to ferric reducing antioxidant power in plasma from model laboratory animal (Grey Partridge) and comparison to standard antioxidants

Miroslav Pohanka1,*, Hana Banďouchová3, Kristina Vlčková1, Jana Žďárová Karasová1, Kamil Kuča1,2, Veronika Damková3, Lucie Pecková3, František Vitula3, Jiří Pikula3
1 Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic
2 University Hospital, Hradec Králové, Czech Republic
3 University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic

Low molecular weight antioxidants (LMWAs) were assayed by square wave voltammetry (SWV) using screen printed electrodes. Standard antioxidants, i.e. uric acid, ascorbic acid, trolox and glutathione, were assayed in order to estimate the sensitivity and standard redox potentials of individual LMWAs. In another experiment, plasma from Grey Partridges was used as model real samples. Ferric reducing antioxidant power (FRAP) was used as a reference method. Two peaks in plasma samples were found by SWV and correlated to FRAP. The SWV peaks were successfully correlated to FRAP. The practical importance of SWV carried out on screen printed electrodes is discussed.

Keywords: electrochemistry; antioxidant assay; oxidative stress; reactive oxygen species; avian blood; biochemistry

Received: May 24, 2010; Revised: July 1, 2010; Published: July 31, 2011  Show citation

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Pohanka M, Banďouchová H, Vlčková K, Žďárová Karasová J, Kuča K, Damková V, et al.. Square wave voltammetry on screen printed electrodes: comparison to ferric reducing antioxidant power in plasma from model laboratory animal (Grey Partridge) and comparison to standard antioxidants. J Appl Biomed. 2011;9(2):103-109. doi: 10.2478/v10136-009-0032-6.
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