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In vitro regeneration of Persian poppy (Papaver bracteatum)

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Abstract

Persian poppy (Papaver bracteatum Lindl.) is an important commercial source of medicinal opiates and related compounds. In this research, calli were induced from seeds, roots, cotyledons and hypocotyls of P. bracteatum at a high efficiency. The optimized callus induction media consisted of the Murashige and Skoog (MS) basic media supplemented with 1.0 mg/L 2, 4-dichlorophenoxyacetic acid (2,4-D), 0.1 mg/L kinetin and 15 mg/L ascorbic acid. The concentrations of 2,4-D and ascorbic acid were found critical to callus induction and proliferation. Subsequent subcultures resulted in excellent callus proliferation. Ascorbic acid at concentration 15 mg/L increased the callus proliferation significantly. Maximum callus growth was achieved when the explants were incubated at 25°C. MS salts at full strength were found inhibitory for callus induction, while ľ MS salts were found to favor callus induction. Shoot regeneration of calli in vitro was achieved on ľ MS medium containing 0.5 mg/L benzylamine purine and 1.0 mg/L naphthalene acetic acid. Analysis of alkaloid extracts from Persian poppy tissues by high-performance liquid chromatography showed that thebaine accumulated in the tissues of plants. The thebaine alkaloid profile of the Persian poppy is a well-defined model to evaluate the potential for metabolic engineering of thebaine production in P. bracteatum.

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Abbreviations

BA:

benzylamine purine

2,4-D:

2,4-dichlorophenoxyacetic acid

DMR:

Duncan’s multiple range

HPLC:

high-performance liquid chromatography

MS:

Murashige and Skoog

NAA:

α-naphthalene acetic acid

SD:

standard deviation

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Authors and Affiliations

  1. Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, Iran

    Sara Rostampour & Haleh Hashemi Sohi

  2. Department of Agronomy and Plant Breeding, University of Zabol, Zabol, Iran

    Sara Rostampour

  3. Department of Plant Breeding, Faculty of Agronomic Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

    Ali Dehestani

Authors
  1. Sara Rostampour
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  2. Haleh Hashemi Sohi
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  3. Ali Dehestani
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Rostampour, S., Sohi, H.H. & Dehestani, A. In vitro regeneration of Persian poppy (Papaver bracteatum). Biologia 65, 647–652 (2010). https://doi.org/10.2478/s11756-010-0079-6

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