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Effect of fenitrothion on Spodoptera exigua larval development and ultrastructure of follicle cells

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Abstract

The effect of four sublethal concentrations (from 0.01165 to 0.3025 µg per insect, responding to LC10/10, LC10, LC30 and LC50) of the organophosphorus insecticide, fenitrothion, on Spodoptera exigua (Lepidoptera, Noctuidae) larval development and ultrastructure of follicular cells of adult female moths was tested. The most prominent malformations were: breaks in the cuticle, increased mortality during larval/pupal molting, alterations of nuclear envelope, changed heterochromatin pattern and abnormalities in mitochondrial ultrastructure. Some of the alterations are dose-dependent, but the other ones, like organization of rough endoplasmic reticulum (RER), show non-linear response: cells exposed to lower concentrations (LC10/10 and LC10) of the insecticide possessed clusters of accumulated RER, which were absent within the cells exposed to higher ones (LC30 and LC50). Formation of the eggshell was also markedly postponed within the groups exposed to lower doses. These findings prove that even minute amounts of xenobiotics may cause significant changes within exposed populations.

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Abbreviations

LC x :

concentration of the insecticide which is lethal for x% of the population

RER:

rough endoplasmic reticulum

SEM:

scanning electron microscope, scanning electron microscopy

TEM:

transmission electron microscope, transmission electron microscopy

References

  • Adamski Z. 2007. Exposure to carbaryl leads to ultrastructural changes and alters the activity of antioxidant enzymes in Spodoptera exigua (Lepidoptera: Noctuidae). Inv. Biol. 126: 191–201.

    Article  Google Scholar 

  • Adamski Z., Banaszkiewicz M. & Ziemnicki. K. 2005a. Ultrastructural and developmental alterations in larvae of Tenebrio molitor L. (Insecta, Coleoptera) induced by sublethal concentrations of fenitrothion. J. Biol. Res. 3: 15–22.

    CAS  Google Scholar 

  • Adamski Z., Fila K., Maćkowiak J. & Ziemnicki K. 2005b. Fenitrothion-induced cell malformations in vitro in Spodoptera exigua cell line UCR-Se-1. Biol. Lett. 42: 41–47.

    CAS  Google Scholar 

  • Adamski Z., Niewadzi M. & Ziemnicki K. 2005c. Inheritance of chorionic malformations and insecticide resistance by Spodoptera exigua. J. Appl. Entomol. 129: 526–533.

    Article  CAS  Google Scholar 

  • Adamski Z. & Ziemnicki K. 2004. Side effects of mancozeb on Spodoptera exigua (Hübn.) larvae. J. Appl. Entomol. 128: 212–217.

    CAS  Google Scholar 

  • Antunes-Madeira. M. & Madeira. V. 1979. Interaction of insecticides with lipid membranes. Biochim. Biophys. Acta 550: 384–392.

    Article  PubMed  CAS  Google Scholar 

  • Choi J., Roche H. & Caquet T. 2001. Hypoxia, hyperoxia and exposure to potassium dichromate or fenitrothion alter the energy metabolism in Chironomus riparius Mg. (Diptera: Chironomidae) larvae. Comp. Biochem. Physiol. 130C: 11–17.

    CAS  Google Scholar 

  • Clos M.V., Ramoneda M. & Garcia G. 1994. Modification of testicular cytochrome P-450 after fenitrothion administration. Gen. Pharmacol. 25: 499–503.

    PubMed  CAS  Google Scholar 

  • David W., Ellaby S. & Taylor G. 1975. Rearing Spodoptera exempta on semi-synthetic diets and on growing maize. Entomol. Exp. Appl. 19: 226–236.

    Article  Google Scholar 

  • Domenech C.E., Machado de Domenech E.E. & Balegno H.F. 1977. Pesticide action and membrane fluidity. FEBS Lett. 74: 243–246.

    Article  PubMed  CAS  Google Scholar 

  • Fila K., Adamski Z. & Ziemnicki K. 2002. Exposure to fenitrothion causes malfunctions of Spodoptera exigua Hübn. (Lepidoptera: Noctuidae) eggs. J. Appl. Entomol. 126: 114–118.

    Article  CAS  Google Scholar 

  • Finney D.J. 1971. Probit Analysis. Cambridge University Press, Cambridge, 333 pp.

    Google Scholar 

  • Fitter A.H. & Fitter R.S.R. 2002. Rapid changes in flowering time in British plants. Science 296: 1689–1691.

    Article  PubMed  CAS  Google Scholar 

  • Garaj-Vrhovac V. & Zeljezic. D. 2002. Assessment of genome damage in a population of Croatian workers employed in pesticide production by chromosomal aberration analysis, micronucleus assay and Comet assay. J. Appl. Toxicol. 22: 249–255.

    Article  PubMed  CAS  Google Scholar 

  • Hagar H.H., Azza H. & Fahmy. 2002. A biochemical, histochemical, and ultrastructural evaluation of the effect of dimethoate intoxication on rat pancreas. Toxicol. Lett. 133: 161–170.

    Article  PubMed  CAS  Google Scholar 

  • Lemos W.P., Medeiros R.S., Zanuncio J.C. & Serrao J.E. 2005. Effect of sub-lethal concentrations of permethrin on ovary activation in the predator Supputius cincticeps (Heteroptera: Pentatomidae) Braz. J. Biol. 65: 287–290.

    CAS  Google Scholar 

  • Li H. & Zhang S. 2001. In vitro cytotoxicity of the organophosphorus pesticide parathion to FG-9307 cells. Toxicol. In Vitro 15: 643–647.

    Article  PubMed  CAS  Google Scholar 

  • Lycett G.J., McLaughlin L.A., Ranson H., Hemingway J., Kafatos F.C., Loukeris T.G. & Paine M.J. 2006. Anopheles gambiae P450 reductase is highly expressed in oenocytes and in vivo knockdown increases permethrin susceptibility. Insect Mol. Biol. 15: 321–327.

    Article  PubMed  CAS  Google Scholar 

  • Margaritis L.H. 1985. Structure and physiology of the eggshell, pp. 151–230. In Gilbert L.I. & Kercut G.A. (eds), Comprehensive Insect Physiology, Biochemistry and Pharmacology, vol. 1. Pergamon, Oxford, New York.

    Google Scholar 

  • Mehlhorn H., Hansen O. & Mencke N. 2001. Comparative study on the effects of three insecticides (fipronil, imidacloprid, selamectin) on developmental stages of the cat flea (Ctenocephalides felis Bouche 1835): a light and electron microscopic analysis of in vivo and in vitro experiments. Parasitol. Res. 87: 198–207.

    Article  PubMed  CAS  Google Scholar 

  • Mehlhorn H., Mencke N. & Hansen O. 1999. Effects of imidacloprid on adult and larval stages of the flea Ctenocephalides felis after in vivo and in vitro application: a light- and electronmicroscopy study. Parasitol. Res. 85: 625–637.

    Article  PubMed  CAS  Google Scholar 

  • Moretti M., Marcarelli M., Villarini M., Fatigoni C., Scassellati-Sforzolini G. & Pasquini R. 2002. In vitro testing for genotoxicity of the herbicide terbutryn, cytogenetic and primary DNA damage. Toxicol. In Vitro 16: 81–88.

    Article  PubMed  CAS  Google Scholar 

  • Nath B.S., Suresh A., Varma B.M. & Kumar R.P.S. 1997. Changes in protein metabolism in hemolymph and fat body of the silkworm, Bombyx mori (Lepidoptera, Bombycidae) in response to organophosphorus insecticides toxicity. Ecotoxic. Environ. Saf. 36: 169–173.

    Article  CAS  Google Scholar 

  • Sancho E., Ferrando M.D. & Andreu E. 1997. Sublethal effects of an organophosphate insecticide on the European eel, Anguilla anguilla. Ecotoxic. Environ. Saf. 36: 57–65.

    Article  CAS  Google Scholar 

  • van der Woude H., Alink G.M. & Rietjens I.M. 2005. The definition of hormesis and its implications for in vitro to in vivo extrapolation and risk assessment. Crit. Rev. Toxicol. 35: 463–582.

    Article  Google Scholar 

  • Visser M.E., Holleman L.J. & Gienapp P. 2006. Shifts in caterpillar biomass phenology due to climate change and its impact on the breeding biology of an insectivorous bird. Oecologia 147: 164–172.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Animal Physiology and Developmental Biology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, ul. Umultowska 89, 61-614, Poznań, Poland

    Zbigniew Adamski, Daria Korolczuk, Magdalena Purgiel, Anna Grewling, Michał Bankiet & Kazimierz Ziemnicki

  2. Electron and Confocal Microscope Laboratory, Faculty of Biology, Adam Mickiewicz University, ul. Umultowska 89, 61-614, Poznań, Poland

    Zbigniew Adamski & Marlena Ratajczak

Authors
  1. Zbigniew Adamski
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  2. Daria Korolczuk
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  3. Magdalena Purgiel
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  4. Anna Grewling
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  5. Marlena Ratajczak
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  6. Michał Bankiet
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  7. Kazimierz Ziemnicki
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Correspondence to Zbigniew Adamski.

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Adamski, Z., Korolczuk, D., Purgiel, M. et al. Effect of fenitrothion on Spodoptera exigua larval development and ultrastructure of follicle cells. Biologia 64, 197–202 (2009). https://doi.org/10.2478/s11756-009-0022-x

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  • DOI: https://doi.org/10.2478/s11756-009-0022-x

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