Abstract
The partitioning of acetylspiramycin was carried out in an aqueous two-phase system (ATPS) formed by a hydrophilic ionic liquid (1-butyl-3-methylimidazolium tetrafluoraborate, [Bmim]BF4) and NaH2PO4. This ATPS is a simple, non-toxic and effective sample pretreatment technique, which was developed for the simultaneous separation, enrichment and rapid analysis of acetylspiramycin coupled with molecular fluorescence spectrophotometry. Analysis of the liquid-liquid equilibrium of [Bmim]BF4-salt ATPS demonstrated that the salting-out ability of different salts may be related to the Gibbs energy of hydration of the ions. The effects of types of salts, concentration of NaH2PO4, and temperature were analysed. Under optimum conditions, the average extraction efficiency and partition coefficient were 90.14% and 91.1, respectively. Thermodynamic functions provide some information about the molecular mechanism involved in acetylspiramycin transfer to the top phase, suggesting an important acetylspiramycin-[Bmim]BF4 interaction. The method yielded a linear range in the concentration from 1.0 to 10.0 µg mL−1 of acetylspiramycin, and the limit of detection was 0.02 µg mL−1. This method could be successfully applied for the analysis of acetylspiramycin in lake water, river water and groundwater. The proposed extraction technique appears to be suitable as a first step for the separation of macrolide antibiotics from real aqueous environments.
[1] T.C. Lo, In: P.A. Schwietzer (Ed.), Handbook of Separations Techniques for Chemical Engineers (McGraw-Hill, New York, 1996) Search in Google Scholar
[2] R.D. Noble, R. Agrawal, Ind. Eng. Chem. Res. 44, 2887 (2005) http://dx.doi.org/10.1021/ie050147510.1021/ie0501475Search in Google Scholar
[3] J. Rydberg, M. Cox, C. Musikas, G. Choppin, Solvent Extraction Principles and Practice, 2nd Edition (Marcel Dekker, New York, 2004) Search in Google Scholar
[4] H.Q. Yin, M. Mao, J.B. Huang, H.L. Fu, Langmiur 18, 9198 (2002) http://dx.doi.org/10.1021/la025902n10.1021/la025902nSearch in Google Scholar
[5] J. Chen, G.X. Ma, D.Q. Li, Prep. Biochem. Biotechnol. 29, 371 (1999) http://dx.doi.org/10.1080/1082606990854493510.1080/10826069908544935Search in Google Scholar
[6] R.D. Rogers, C.B. Bauer, J. Chromatogr. B. 680, 237(1996) http://dx.doi.org/10.1016/0378-4347(95)00319-310.1016/0378-4347(95)00319-3Search in Google Scholar
[7] H.D. Willauer, J.G. Huddleston, R.D. Rogers, Ind. Eng. Chem. Res. 41, 1892 (2002) http://dx.doi.org/10.1021/ie010598z10.1021/ie010598zSearch in Google Scholar
[8] M.R. Helfrich, M. EI-Kouedi, M.R. Etherton, C.D. Keating, Langmuir 21, 8478 (2005) http://dx.doi.org/10.1021/la051220z10.1021/la051220zSearch in Google Scholar
[9] J. Chen, S.K. Spear, J.G. Huddleston, R.D. Rogers, Green Chem. 7, 64 (2005) http://dx.doi.org/10.1039/b413546f10.1039/b413546fSearch in Google Scholar
[10] J. Chen, S.K. Spear, J.G. Huddleston, J.D. Holbrey, R.P. Swatloski, R.D. Rogers, Ind. Eng. Chem. Res. 43, 5358 (2004) http://dx.doi.org/10.1021/ie034149610.1021/ie0341496Search in Google Scholar
[11] I.H. Pan, H.H. Chui, C.H. Lu, L.T. Lee, Y.K. Li, J. Chromatogr. A. 977, 239 (2002) http://dx.doi.org/10.1016/S0021-9673(02)01385-710.1016/S0021-9673(02)01385-7Search in Google Scholar
[12] K.E. Gutowski, G.A. Broker, H.D. Willauer, J.G. Huddleston, R.P. Swatloski, J.D. Holbrey, R.D. Rogers, J. Am. Chem. Soc. 125, 6632 (2003) http://dx.doi.org/10.1021/ja035180210.1021/ja0351802Search in Google Scholar PubMed
[13] C. He, S. Li, H. Liu, K. Li, F. Liu, J. Chromatogr. A. 1082, 143 (2005) http://dx.doi.org/10.1016/j.chroma.2005.05.06510.1016/j.chroma.2005.05.065Search in Google Scholar
[14] S. Li, C. He, H. Liu, K. Li, F. Liu, J. Chromatogr. B. 826, 58 (2005) http://dx.doi.org/10.1016/j.jchromb.2005.08.00510.1016/j.jchromb.2005.08.005Search in Google Scholar
[15] Z. Du, Y. Yu, J. Wang, Chem. Eur. J. 13, 2130 (2007) http://dx.doi.org/10.1002/chem.20060123410.1002/chem.200601234Search in Google Scholar
[16] Q.F. Liu, J. Yu, W.L. Li, X.S. Hu, H.S. Xia, H.Z. Liu, P. Yang, Sep. Sci. Technol. 41, 2849 (2006) http://dx.doi.org/10.1080/0149639060078613510.1080/01496390600786135Search in Google Scholar
[17] A. Soto, A. Arce, M.K. Khoshkbarchi, Sep. Purif. Technol. 44, 242 (2005) http://dx.doi.org/10.1016/j.seppur.2005.01.01310.1016/j.seppur.2005.01.013Search in Google Scholar
[18] National Pharmacopoeia Committee of China, Pharmacopoeia of the People’s Republic of China (Chemical Engineering Press Beijing, China, 2005) vol. II Search in Google Scholar
[19] S. Omura, Macrolide Antibiotics: Chemistry, Biology and Practice (Academic Press, Orlando, 1984) 26 Search in Google Scholar
[20] M. Horie, H. Takegami, K. Toya, H. Nakazawa, Anal. Chim. Acta 492, 187 (2003) http://dx.doi.org/10.1016/S0003-2670(03)00891-210.1016/S0003-2670(03)00891-2Search in Google Scholar
[21] The State Pharmacopoeia Commission of PR China, Pharmacopoeia of the People’s Republic of China Edition 2000 (Chemical Industry Press, Beijing, 2000) Search in Google Scholar
[22] B.B. Ivanova, Spectrochimi. Acta, Part A 62, 58(2005 http://dx.doi.org/10.1016/j.saa.2004.12.01110.1016/j.saa.2004.12.011Search in Google Scholar PubMed
[23] B.B. Koleva, T. Kolev, R.W. Seidel, H. Mayer-Figge, M. Spiteller, W.S. Sheldrick, J. Phys. Chem. A 112, 2899 (2008) http://dx.doi.org/10.1021/jp710765v10.1021/jp710765vSearch in Google Scholar PubMed
[24] Y. Pei, J. Wang, L. Liu, K. Wu, Y. Zhao, J. Chem. Eng. Data. 52, 2026 (2007) http://dx.doi.org/10.1021/je700315u10.1021/je700315uSearch in Google Scholar
[25] Y. Marcus, J. Chem. Soc., Faraday Trans. 89, 713 (1993) http://dx.doi.org/10.1039/ft993890071310.1039/FT9938900713Search in Google Scholar
[26] Y. Marcus, J. Chem. Soc., Faraday Trans. 87, 2995 (1991) http://dx.doi.org/10.1039/ft991870299510.1039/FT9918702995Search in Google Scholar
[27] M.T. Zafarani-Moattar, S. Hamzehzadeh, J. Chem. Eng. Data. 54, 833 (2009) http://dx.doi.org/10.1021/je800625910.1021/je8006259Search in Google Scholar
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