Impact of AKT1 polymorphism on DNA damage, BTG2 expression, and risk of colorectal cancer development
Article Category: research article
Published Online: Aug 14, 2022
Page range: 336 - 345
Received: Feb 27, 2022
Accepted: Jul 03, 2022
DOI: https://doi.org/10.2478/raon-2022-0031
© 2022 Hina Zubair, Zahid Khan, Muhammad Imran, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Background
AKT, also called protein kinase B, is a serine-threonine kinase that functions as a mediator of PI3K-Akt-mTOR signaling pathway and plays an important role in an array of cellular processes. Many single nucleotide polymorphisms (SNP) in
Patients and methods
A total 197 population-based controls and 200 CRC patients were genotyped for SNP rs1130233. AKT expression, activation and
Results
The heterozygous AG genotype (55.67%) was more prevalent in the local population compared to homozygous wild type GG (37.78%) and homozygous AA genotypes (6.55%). Moreover, AG and AA alleles were observed to be significant contributors (P = 0.01, OR = 1.80, CI = 1.18 to 2.74, and P = 0.001, OR = 5.00, CI = 1.90 to 13.18, respectively) in increasing the risk of CRC. The immunoblot analysis revealed that G to A transition decreased the expression and activation of AKT. Moreover, AG and AA genotypes of
Conclusions
The data concludes that G to A substitution is a risk factor for CRC development involving a decrease in AKT expression and activation and increase in DNA damage.